Sophie Laguesse date 2016/05/4 experiment number SL133 Aim: F-G actin assay in Nac infused with ltv-shProsapip1 + test of ltv-shRNA in vivo after CPP experiment Method: Ltv- ShScr or Ltv-shProsapip-1 (03.03) were infused into the Nac of mice. 3 weeks later, Nac were dissected (GFP) and lyzed into RIPA buffer. Prosapip1, GAPDH and GFP expression were determined by WB. Mice are NM066. GFP SCR11 Sh 15 Prosapip1 Actin SCR10 Sh 13 SCR20 Sh 14 SCR12 Sh 16 ) o l t r Prosapip1 o n c 150 h e t o f % 100 ( *** H D P A G 50 1 / p p i o s a r C R 1 P h S a p i p s s P r o s h s h S C R P r o a p i 1 50 100 150 F - c t n / l ( % f e ) ** F-actin s h S C R P r o a p i 1 50 100 150 G-actin F - c t n / l ( % f e ) ** SCR10 Sh 13 SCR20 Sh 14 G F Prosapip1 Actin SCR 12 Sh 16 (Stats for n=5 SL128+SL133)

Sophie Laguesse date 2016/04/15 experiment number SL128 Aim: F-G actin assay in Nac infused with ltv-shProsapip1 + test of ltv-shRNA in vivo before CPP experiment Method: Ltv- ShScr or Ltv-shProsapip-1 (03.03) were infused into the Nac of mice. 3 weeks later, Nac were dissected (GFP) and lyzed into RIPA buffer. Prosapip1, GAPDH and GFP expression were determined by WB. Virus tested in N2A cells in SL105 GFP SCR2 Sh 5 Prosapip1 Actin SCR1 Sh 6 SCR3 Sh 7 SCR4 Sh 8 Method: F-G actin assay according to the protocol on SCR1, sh6, SCR3, sh7 SCR1 Sh 6 SCR3 Sh 7 G F G F Actin G F G F Prosapip1  Decreased F-actin in shProsapip1 Nac. Need to increase the n