Horizon Chromatography HPLC/UHPLC Columns

Introduction Horizon new HPLC, UHPLC and Core-Shell ranges Ultra pure silica 99.999% High surface area (300m2/g) Excellent peak shapes High resolution selectivity choices We pride ourselves on our delivery and sales service Technical Support service

Available Phases Aurashell – Core-Shell Aurashell C18 Horizon – Fully Porous (300m2/g) Aurashell C18 Aurashell C18/PFP Aurashell Aqua C18 Aurashell HILIC Aurashell PEP C18 (160Å) Aurashell PRO C4 (300Å) Horizon C18 Horizon C18/PFP Horizon Aqua C18 Horizon C8 Horizon HILIC Horizon Phenyl Horizon Amino Horizon Cyano

Physicals Ultra pure 99.999% silica template All phases are extensively endcapped Horizon, porous particles 1.6µm UHPLC particles 3µm High efficiency particle 5µm standard ‘work horse’ particle Aurashell, core-shell 2.7µm ultra high efficiency, low backpressure 5µm high efficiency, low backpressure

Physicals S.A. %C Pore Size pH range USP Purity Endcapped Horizon C18 300m2/g 20 120Å 1-11 L1 99.999% Yes Horizon Aqua 16 2-10 Horizon C18/PFP 15 Horizon C8 12 L7 Horizon Phenyl 2-8 L11 Horizon Cyano 7 1-6 L10 Horizon HILIC 4 L20 Horizon Amino 5 L8

Aurashell Aurashell Monodisperse core shell particle High speed separations Uniform packed bed, low backpressure Multiple particle sizes 2.7µm 5µm Reversed phase chemistries Bioanalysis optimised phases

UHPLC 1.6µm Horizon particles for UHPLC Fully porous ultra pure inert silica Excellent peak shapes and reproducibility High efficiency UHPLC results Multiple orthogonal selectivity's available

Horizon C18 Horizon C18 HPLC and UHPLC particle sizes Excellent performance general purpose C18 alkyl chain stationary phase with high stability Excellent peak shapes Excellent selectivity Guaranteed batch to batch reproducibility

Horizon C18 Horizon C18 Ultra high purity silica 99.999% 300m2/g surface area 120Å pore size pH range 1-11 USP : L1

Horizon C18 Column: Horizon C18 3µm 150x4.6mm Mobile Phase: A: 10mM Ammonium Bicarbonate pH10 B: MeOH Gradient : 60:40 – 100% B in 10minutes Flow rate : 1ml/min Wavelength: 220nm Temperature: 30oC Tramadol Amitriptyline

Horizon C18 Column: Horizon C18 3µm 150x4.6mm Mobile Phase: A: 10mM Ammonium formate pH3.5 B: MeCN Gradient : 60:40 – 100% B in 10minutes Flow rate : 1ml/min Wavelength: 254nm Temperature: 20oC Rosuvastatin Simvastatin Atorvastatin

Horizon C18/PFP Horizon C18/PFP HPLC and UHPLC particle sizes Alternative selectivity L1 columns Offers strong hydrophobic, pi-pi and dipole interactions Extend retention of halogenated compounds Guaranteed batch to batch reproducibility

Horizon C18/PFP Horizon C18/PFP Ultra high purity silica 99.999% 300m2/g surface area 120Å pore size pH range 2-10 USP : L1

Horizon C18/PFP 1,2,3 5,6 4 Traditional C18 7 8 3 4 5 Horizon C18/PFP Column: 3µm 150x4.6mm Mobile Phase: 50:50 Water: MeOH Flow rate : 1ml/min Wavelength: 254nm Temperature: 40oC 1,2,3 5,6 4 Traditional C18 7 8 3 4 1,2,3-Trimethoxybenzene 1,2,4-Trimethoxybenzene 1,2-Dimethoxybenzene 1,4-Dimethoxybenzene Methoxybenzene 1,3-Dimethoxybenzene 1,3,5-Trimethoxybenzene Toluene 5 Horizon C18/PFP 6 2 7 8 1

Horizon C18/PFP Column: Horizon C18/PFP 3µm 150x4.6mm Mobile Phase: A: 0.1% Formic acid B: 0.1% Formic acid in MeCN Gradient : 10 – 100% B in 10minutes Flow rate : 1ml/min Wavelength: 254nm Temperature: 20oC Paracetamol Hydrochlorothiazide Methylphenylsulfoxide Methylphenylsulfone

Horizon AQUA C18 Horizon Aqua C18 HPLC and UHPLC particle sizes Excellent performance C18 with capability to operate in 100% aqueous mobile phases Resistant to retention loss caused by highly aqueous mobile phases Analyse polar compounds

Horizon AQUA C18 Horizon Aqua C18 Ultra high purity silica 99.999% 300m2/g surface area 120Å pore size pH range 2-10 USP : L1

Horizon AQUA C18 Column: Horizon Aqua C18 5µm 150x4.6mm Mobile Phase: A: 50mM KH2PO4 pH 3.5 B: MeOH Gradient : 0 – 100% B in 15minutes Flow rate : 1ml/min Wavelength: 205nm Temperature: 30oC Vitamin B1 Vitamin B6 Vitamin B3 Vitamin B5 Folic acid Vitamin B12 Vitamin H Vitamin B2

Horizon AQUA C18 Column: Horizon Aqua C18 5µm 150x4.6mm Mobile Phase: A: 0.1% TFA B: 0.1% TFA in MeOH Gradient : 0 – 100% B in 15minutes Flow rate : 1ml/min Wavelength: 270nm Temperature: 20oC Dopamine Serotonin DOPAC

Peptide/Protein Columns Horizon BIO phases Horizon fully porous PEP C18 and PRO C4 PEP C18 = 200Å C18 designed for peptide analysis and mapping PRO C4 = 300Å C4 for wide range of protein analysis Aurashell PEP C18 2.7µm core shell 160Å pore size High efficiency phase for peptides Aurashell PRO C4 3.5µ, core shell 300Å pore size High efficiency phase for proteins

Aurashell PEP C18 Aurashell PEP C18 Monodisperse 160Å pore size C18 ligand (6% C) pH range 1-9 USP : L1

Aurashell PEP C18

Aurashell PEP C18 Tryptic digest 2.7µm Aurashell PEP C18 150x2.1mm 60 minute gradient 30 minute gradient 20 minute gradient

Aurashell PRO C4 Aurashell PRO C4 Monodisperse 300Å pore size C4 ligand (1% C) pH range 1-9 USP : L26

Aurashell PRO C4

Aurashell PRO C4 Protein Mix 3.5µm Aurashell PRO C4 100x2.1mm Ribonuclease A (13.7kDa) Cytochrome C (12kDa) Halo-Transferrin (77kDa) Apomyoglobin (17kDa)

Other Dimensions Preparative Columns 10mm or 21.2mm diameters Ultra High purity 5µm or 10µm particles High surface area High sample loading Excellent sample recovery Capillary 75µm i.d. Available with Horizon or Aurashell particles Full range of bonded phases Standard and custom made lengths available

Guards and Filters Horizon Guards: Significantly increase the lifetime of columns Prevent contamination Available in all phase chemistries Horizon Analytical pre-column filter: 2µm porosity frit 400bar pressure rated Finger tight Horizon UHPLC pre-column filter: 0.5µm porosity frit 1000bar pressure rated Compatible with all UHPLC systems

Conclusion Horizon phases offer: Selectivity Efficiency Excellent peak shapes UHPLC 1.6µm particles Aurashell phases offer: High Efficiency High Resolution High Speed

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