Figure 1. BRAF V600E mutation analysis in PTC

Slides:

Advertisements

Similar presentations

Supplementary data Fig 1: Comparison of differential mRNA expression data obtained by qRT-PCR and microarray (relative expression in tumors compared to.

Advertisements

Is the BRAF V600E mutation useful as a predictor of preoperative risk in papillary thyroid cancer? The American Journal of Surgery.

About these slides SPEC – Short Presentation in Emerging Concepts Provided by the CAP as an aid to pathologists to facilitate discussion on the topic.

Date of download: 7/9/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Follicular Variant of Papillary Thyroid Cancer: Encapsulated,

Copyright © 2012 American Medical Association. All rights reserved.

The utility of the Bethesda category and its association with BRAF mutation in the prediction of papillary thyroid cancer stage Augustas Beiša1, Mindaugas.

Invasive follicular variant of papillary thyroid cancer harboring the NRAS mutation Q61K and presenting with bone metastasis—A case report Raman Mehrzad,

Fig. 1. Kaplan-Meir survival curves for Stoke Cushing's patients, with follow-up duration in years on the x-axis. Number of patients in each subgroup is.

Fig. 1. Mean BMI and BMI Z-score (se) of the children with AA, TA, and TT genotypes in rs of the FTO gene. The P values are for the additive model.

Fig. 1. Correlation of TSH with TC, LDL-C, HDL-C, and TG.

Figure 2 Top panel shows mean (SE) differences in near-final height (cm) (n = 71) in the AI, GH, and AI/GH groups regardless of length of treatment (n.

Quantified net intensity data are in arbitrary units (AU)

Fig. 1. Mean plasma lipid and lipoprotein levels as a function of the common genetic variants in LCAT, g.-293G>A, S208T, and L369L in the CCHS and S208T.

Figure 1. Total (A) and free (B) T, estradiol (C), and SHBG (D) levels at baseline and during treatment. From: Testosterone Treatment and Sexual Function.

Figure 1. Summary of PTH secretion from human parathyroid cells after caffeine treatment at concentrations of 0, 1, 10, and 50 μm at extracellular calcium.

Predicted values and SEs were based on linear mixed models

Fig. 3. Mean body weight of women randomized to low-carbohydrate and low-fat diets over the course of the 4-month trial. The first time point (wk 1) represents.

From: Is Age a Risk Factor for Hypothyroidism in Pregnancy

Fig. 1. Trial profile. From: Randomized Teriparatide [Human Parathyroid Hormone (PTH) 1–34] Once-Weekly Efficacy Research (TOWER) Trial for Examining the.

Figure 4. Inhibition of LPS-induced IL-1β and TNFα, but not IL-6, after exercise. Depicted are LPS-induced IL-1β (upper panel), IL-6 (middle panel), and.

Fig. 4. Relationships of changes in CRP or SAA to changes in body weight and insulin resistance. For each comparison the regression line is shown (solid.

Y - Tests Type Based on Response and Measure Variable Data

B) a) Figure S4. Dynamic changes of DNA methylation in the SPDEF promoter during goblet cells differentiation of PBECs from patients with COPD. a) PBECs.

Evaluation of the Real-Q BRAF V600E Detection Assay in Fine-Needle Aspiration Samples of Thyroid Nodules Kyung Sun Park, Young L. Oh, Chang-Seok Ki,

SA3202 Statistical Methods for Social Sciences

MicroRNAs for the Prediction of Early Response to Sorafenib Treatment in Human Hepatocellular Carcinoma Liver Cancer 2017;6: DOI: /

A B C Supplementary figure S7

Hendrikus J. Dubbink, Peggy N. Atmodimedjo, Ronald van Marion, Niels M

A Quantitative Allele-Specific PCR Test for the BRAF V600E Mutation Using a Single Heterozygous Control Plasmid for Quantitation Philippe Szankasi, N.

Association of functional genetic variants of CTLA4 with reduced serum CTLA4 protein levels and increased risk of idiopathic recurrent miscarriages Maneesh.

WT G>A G>C G>T (n = 93) (n = 21) (n = 10) (n = 51)

Impaired function of human T-lymphotropic virus type 1 (HTLV-1)–specific CD8+ T cells in HTLV-1–associated neurologic disease by Amir H. Sabouri, Koichiro.

Basics of Immunohistochemistry

Gene Dysregulations Driven by Somatic Copy Number Aberrations-Biological and Clinical Implications in Colon Tumors Manny D. Bacolod, Francis Barany

Total-Genome Analysis of BRCA1/2-Related Invasive Carcinomas of the Breast Identifies Tumor Stroma as Potential Landscaper for Neoplastic Initiation

Comparison of Allelic Discrimination by dHPLC, HRM, and TaqMan in the Detection of BRAF Mutation V600E Pablo Carbonell, María C. Turpin, Daniel Torres-Moreno,

Clinical Relevance of Sensitive and Quantitative STAT3 Mutation Analysis Using Next- Generation Sequencing in T-Cell Large Granular Lymphocytic Leukemia

Skin-Resident Effector Memory CD8+CD28– T Cells Exhibit a Profibrotic Phenotype in Patients with Systemic Sclerosis Gang Li, Adriana T. Larregina, Robyn.

Intraoperative detection of lymph node micrometastasis with flow cytometry in non– small cell lung cancer Manabu Ito, MD, Yoshihiro Minamiya, MD, PhD,

Nicolas Jacquelot, María Paula Roberti, David P

Proteome Profiling of Human Cutaneous Leishmaniasis Lesion

by Atsuhiko Hasegawa, Huining Liu, Binhua Ling, Juan T

Dorien Van Saen, Ph. D. , Ellen Goossens, Ph. D. , Joeri L. Aerts, Ph

IL-36γ (IL-1F9) Is a Biomarker for Psoriasis Skin Lesions

Vincent B. McGinty, Antonio Rangel, William T. Newsome Neuron

Volume 7, Issue 4, Pages (April 2005)

Volume 7, Issue 4, Pages (April 2005)

Expression of erythropoietin messenger ribonucleic acid in wild-type MED12 uterine leiomyomas under estrogenic influence: new insights into related growth.

Figure 1. Plasma next-generation sequencing (NGS) assay workflow, comparison of variant allelic fraction with ... Figure 1. Plasma next-generation sequencing.

Floor Weerkamp, MSc, Edwin F. E. de Haas, BSc, Brigitta A. E

Volume 67, Issue 4, Pages (April 2005)

Vikas K. Goel, Alexander J. F. Lazar, Carla L. Warneke, Mark S

Fluorescent In Vivo Detection Reveals that IgE+ B Cells Are Restrained by an Intrinsic Cell Fate Predisposition Zhiyong Yang, Brandon M. Sullivan, Christopher D.C.

Posterior Parietal Cortex Encodes Autonomously Selected Motor Plans

Gene Dysregulations Driven by Somatic Copy Number Aberrations-Biological and Clinical Implications in Colon Tumors Manny D. Bacolod, Francis Barany

Roland Houben, Claudia S. Vetter-Kauczok, Sonja Ortmann, Ulf R

Ly6/uPAR-Related Protein C4

Figure 1 A B GCL NBL ED18.5 PND3 PND6 GCL

Volume 8, Issue 3, Pages (August 2014)

Figure 1 A B GCL NBL ED18.5 PND3 PND6 GCL

Masakazu Hashimoto, Hiroshi Sasaki

Figure 1 A B GCL NBL ED18.5 PND3 PND6 GCL

A ID8 scr sh cx2 cx3 cx6 B C Supplementary Figure 4. The fractalkine axis does not regulate ascites formation.

Figure 1 A B GCL NBL ED18.5 PND3 PND6 GCL

Figure 1 A B GCL NBL ED18.5 PND3 PND6 GCL

The HTT CAG-Expansion Mutation Determines Age at Death but Not Disease Duration in Huntington Disease Jae Whan Keum, Aram Shin, Tammy Gillis, Jayalakshmi Srinidhi.

Pathway-specific tumor suppression

ICOS+ and activated CD4+ T cells are dominant, tumour tissue-specific T cell populations in both mismatch repair-deficient and repair-proficient colorectal.

Fig. 5 Proportions of EpCAM+ systemic tumor cells correlate with the clinical outcome of patients with MBC. Proportions of EpCAM+ systemic tumor cells.

Examine Relationships

Presentation transcript:

Figure 1. BRAF V600E mutation analysis in PTC Figure 1. BRAF V600E mutation analysis in PTC. A, Percentage of mutated (mPTC, PTC CL, PTC FV, PTC TC) and wild-type (WT) samples. PTC CL, PTC-classic; PTC FV, PTC-follicular variant; PTC TC, PTC-tall cell variant. B, Hematoxylin and eosin sections of three representative tumors with BRAF V600E (PTC cases 67, 69, and 65, from left to right) in which the mutation is present in virtually all neoplastic cells. C, Hematoxylin and eosin sections of three representative tumors with BRAF V600E (PTC cases 24, 7, and 6, from left to right) in which the mutation is present in a minority of neoplastic cells. The boxes in panels B and C show the percentage of neoplastic cells in the tumor (Tumor Cell %), the percentage of BRAF V600E-mutated alleles after ASLNAqPCR (Mutated Allele %), and the percentage of BRAF V600E-mutated cells after the normalization to the estimated proportion of neoplastic cells within the tumor (Normalized Mutated Cell %). From: High-Sensitivity BRAF Mutation Analysis: BRAF V600E Is Acquired Early During Tumor Development but Is Heterogeneously Distributed in a Subset of Papillary Thyroid Carcinomas J Clin Endocrinol Metab. 2014;99(8):E1530-E1538. doi:10.1210/jc.2013-4389 J Clin Endocrinol Metab | Copyright © 2014 by the Endocrine Society

Figure 2. Distribution of BRAF V600E-mutated neoplastic cells in PTC and mPTC. A and B, Percentage of BRAF V600E mutated cells before (A) and after (B) normalization to the estimated proportion of neoplastic cells within the tumor in all of the 85 PTC samples. Black bars, tumors greater than 1 cm; white bars, tumors 1 cm or less (mPTC). C and D, Scatter plots showing the percentage of BRAF V600E-mutated neoplastic cells, in all samples (C), and in the mPTC subset (D). Three groups of tumors are recognizable in panels C and D: tumors with less than 30% of BRAF V600E-mutated neoplastic cells, tumors with 30%–80% mutated neoplastic cells, and tumors more than 80% mutated neoplastic cells. Dotted lines indicate 30% and solid lines indicate 80% of BRAF V600E-mutated neoplastic cells, respectively (A–D). E and F, Diagrams showing the distribution of the number of cases according to the percentage of BRAF V600E-mutated neoplastic cells in all samples (E) and in the mPTC subset (F). From: High-Sensitivity BRAF Mutation Analysis: BRAF V600E Is Acquired Early During Tumor Development but Is Heterogeneously Distributed in a Subset of Papillary Thyroid Carcinomas J Clin Endocrinol Metab. 2014;99(8):E1530-E1538. doi:10.1210/jc.2013-4389 J Clin Endocrinol Metab | Copyright © 2014 by the Endocrine Society

Figure 3. BRAF V600E mutational analysis using ASLNAqPCR, NGS, and immunohistochemistry with a BRAF V600E-specific antibody. A, Percentage of BRAF V600E-mutated neoplastic cells in 30 samples after parallel analysis with ASLNAqPCR, and NGS; the dotted line indicates 30% and the solid line indicates 80% of BRAF V600E-mutated neoplastic cells, respectively. B, Correlation between the percentage of BRAF V600E-mutated neoplastic cells established after ASLNAqPCR and NGS analysis (P = .0002, Spearman test). C, Box plot of the percentage of BRAF V600E-mutated neoplastic cells identified after ASLNAqPCR and NGS analysis: there is no statistical difference in the results obtained after ASLNAqPCR and NGS analyses (P = .1064, Wilcoxon signed rank test). D and E, Immunohistochemistry with a BRAF V600E-specific antibody of two representative PTCs with heterogeneous distribution of the BRAF-mutated protein (case 15, D; case 7, E). Mutational analysis of these samples performed by ASLNAqPCR showed a percentage of BRAF V600E-mutated cells after normalization to the estimated proportion of neoplastic cells within the tumor of 36% (case 15, D) and 22.5% (case 7, E), respectively. From: High-Sensitivity BRAF Mutation Analysis: BRAF V600E Is Acquired Early During Tumor Development but Is Heterogeneously Distributed in a Subset of Papillary Thyroid Carcinomas J Clin Endocrinol Metab. 2014;99(8):E1530-E1538. doi:10.1210/jc.2013-4389 J Clin Endocrinol Metab | Copyright © 2014 by the Endocrine Society

Figure 4. Correlation of the percentage of BRAF V600E-mutated neoplastic cells with clinicopathological features. A–C, Tumor size (A): larger tumors tend to have a higher proportion of BRAF-mutated cells; the Spearman test did not reach statistical significance (P = .1121), but ANOVA with the Kruskal-Wallis test showed that tumor size and the proportion of BRAF V600E-mutated cells are statistically associated (P = .012). Patient age (B) (Spearman test, P = .4891; Kruskal-Wallis test, P = .9193) and tumor stage (C) (Spearman test, P = .3089; χ<sup>2</sup> tests, P > .05) are not statistically associated with the percentage of mutated neoplastic cells within the tumor. D and E, Percentage of BRAF V600E-mutated neoplastic cells in PTC cases with (N1) and without (N0) lymph node metastases: there is no statistical association (P = .7172, Mann-Whitney U test). From: High-Sensitivity BRAF Mutation Analysis: BRAF V600E Is Acquired Early During Tumor Development but Is Heterogeneously Distributed in a Subset of Papillary Thyroid Carcinomas J Clin Endocrinol Metab. 2014;99(8):E1530-E1538. doi:10.1210/jc.2013-4389 J Clin Endocrinol Metab | Copyright © 2014 by the Endocrine Society