Page 1 Site-Specific and Custom Labeling — Creative BioMart

Page 2 Creative BioMart is committed to providing advanced tools for protein expression and protein purification. As a leading supplier for reagents in the biotechnology field, we understand the importance of convenient and easy-to-use systems for high level expression and sample purification. We invite you to review our growing range of expression systems resulting from our experience in cloning, overexpression and purification.protein purification

Page 3 Site-Specific Labeling C-terminal labeling N-terminal labeling Labeling at glycosylation sites

Page 4 Especially biomolecule labeling requires reaction procedures that can be performed under physiological conditions (neutral pH, aqueous solution, ambient temperature) with low reactant concentrations to ensure non-toxic, low background labeling at reasonable time scales while still preserving biological function. Among the plethora of possible reactions only a few generally fit the necessary reactivity, selectivity and biocompatibility criteria.

Page 5 Cu-catalyzed Azide-Alkyne reaction Strain-promoted Azide-Alkyne reaction Tetrazine-trans-Cyclooctene Ligation

Page 6 Creative BioMart has extensive experience with various protein labeling techniques and are confident that we can provide you with high-quality labeled reagents for your downstream applications. Possible problems during labeling procedures include protein losses due to precipitation, sample manipulation & instability, inconsistent label-to-protein ratio, incomplete removal of an unconjugated labeling probe, and poor protein characterization before and after labeling. Creative BioMart has new labeling technologies that had emerged for site-directed labeling requiring an integration of protein expression and purification into the labeling process.

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