External Quality Assessment: Choosing an EQA program

Slides:

Advertisements

Similar presentations

Anne Sholander, MT(ASCP), International QA/QC Coordinator, SMILE

Advertisements

QUALITY CONTROL IN HEMATOLOGY

IMMUNOPHENOTYPING OF HEMATOPOIETIC PROGENITOR CELLS BY MULTIPARAMETER FLOW CYTOMETRY.

Deidra Huckabee MSN, RN, CCRN. Research Question Medical ICU staff inquired about the accuracy of utilizing the bedside Point of Care Testing (POCT) analyzer.

Dr Samah Kotb Lecturer of Biochemistry 1 CLS 432 Dr. Samah Kotb Nasr El-deen Biochemistry Clinical practice CLS 432 Dr. Samah Kotb Nasr.

MHRP  The views expressed are those of the authors and should not be construed to represent the positions of the U.S. Army or the Department of Defense.

Flow cytometry to evaluate vaccine-induced T cell responses: standardized analysis of large numbers of FCS files Stephen De Rosa, M.D. HVTN Laboratory.

Automated CBC Parameters

Automated CBC Parameters

Point of Care Testing for MRSA Nigel Brenwald Department of Microbiology Sandwell and West Birmingham Hospitals NHS Trust.

Chapter 4 Summary Scatter diagrams of data pairs (x, y) are useful in helping us determine visually if there is any relation between x and y values and,

Biochemistry Clinical practice CLS 432 Dr. Samah Kotb Lecturer of Biochemistry 2015 Introduction to Quality Control.

Quality control & Statistics. Definition: it is the science of gathering, analyzing, interpreting and representing data. Example: introduction a new test.

Probe Validation Anne Wiktor September 21, Abnormalities identified by FISH.

Course: Research in Biomedicine and Health III Seminar 5: Critical assessment of evidence.

Monitoring and Evaluation in the GMS Learning Program 7 – 18 May 2012, Mekong Institute, Khon Kaen, Thailand Randy S. Balaoro, CE, MM, PMP Data Sampling.

Conclusions Materials and Methods Background Objectives HIV-1 RNA is the most significant determinant of cervical HIV-1 shedding. Shedding has also been.

Diana Careaga 1, Robert Magari 1, Karen Lo 1, Michael Keeney 2, Ben Hedley 2, Dominika Benjamins 2, Dr. You-Wen Qian 3, Larry Hinson 3, Cristina Ceja 3,

Clinical Utility of EQA Dr. Angela Amayo UON27/11/2008.

Patient Safety Monitoring in International Laboratories (SMILE) Mark Swartz, MT(ASCP), SMILE QA/QC Coordinator Improving the Sensitivity of QC Monitoring:

Minimal Residual Disease(MRD) detections by Flow Cytometry in Acute Leukemia 혈액학파트 안 미 숙.

John Gatimu University of Nairobi/University of Washington Research Laboratory What Is Not Mentioned In User Manuals: A Case Study of Quality Control in.

EVALUATING STABILIZATION TUBES FOR STORAGE OF CD4 HIV POSITIVE SAMPLES AT KILIFI DISTRICT HOSPITAL, KENYA Nella Raphael Kalama.

Horace Gumba KEMRI-Wellcome Trust Research Programme

Impact of EQA failure on the Lab Quality Policy

Quality Assurance in the clinical laboratory

Copyright © 2010 American Medical Association. All rights reserved.

JONCKHEERE, Stijn 1; STEUR, Elise 1; BILLIET, Johan 1

Figure 2 Percentage of CD19+/CD34+ cells with decreased CD81 median fluorescence intensity (MFI;

Supplementary Figure S1

Volume 6, Issue 4, Pages (October 2009)

Volume 143, Issue 2, Pages (February 2013)

MRD in Myeloma: the Future is Here

Practical clinical chemistry

Volume 129, Issue 1, Pages (July 2005)

Two cases of disseminated Mycobacterium avium infection associated with a new immunodeficiency syndrome related to CXCR4 dysfunctions A.-V. Doncker,

Volume 134, Issue 4, Pages (April 2008)

A B Supplemental Figure 1 PBMC Cervical Cytobrush

Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.

FLT3 ligand administration after hematopoietic cell transplantation increases circulating dendritic cell precursors that can be activated by CpG oligodeoxynucleotides.

Regulatory T Cells Exhibit Distinct Features in Human Breast Cancer

Tree Net algorithm contruction

Volume 45, Issue 5, Pages (November 2016)

Volume 138, Issue 4, Pages (April 2010)

ALT-803 administration increases peripheral blood cell counts of lymphocyte subsets in cynomolgus monkeys. ALT-803 administration increases peripheral.

Isolating CD8+ T cells from lupus-prone mice.

Volume 20, Issue 9, Pages (August 2017)

Physical exercise modulates the homeostasis of human regulatory T cells Max Weinhold, MA, Alexander Shimabukuro-Vornhagen, MD, Axel Franke, MD, Sebastian.

TSLP Directly Interacts with Skin-Homing Th2 Cells Highly Expressing its Receptor to Enhance IL-4 Production in Atopic Dermatitis Kazuki Tatsuno, Toshiharu.

Supplemental Figure untreated Rituximab Alemtuzumab

Matthew J. Deeths, Bart T. Endrizzi, Michelle L. Irvin, Lynne P

Quality Control Lecture 3

Peter A. Savage, Mark M. Davis Immunity

Biology Standards.

CTLA-4 Blockade following Relapse of Malignancy after Allogeneic Stem Cell Transplantation Is Associated with T Cell Activation But Not with Increased.

Donor antigen-presenting cells regulate T-cell expansion and antitumor activity after allogeneic bone marrow transplantation Jian-Ming Li, Edmund K.

Volume 6, Issue 4, Pages (October 2009)

Quality Assessment The goal of laboratory analysis is to provide the accurate, reliable and timeliness result Quality assurance The overall program that.

Effects of daratumumab on natural killer cells and impact on clinical outcomes in relapsed or refractory multiple myeloma by Tineke Casneuf, Xu Steven.

Figure 2 Peripheral blood lymphocyte subset counts during dimethyl fumarate treatment(A) Lymphocyte subsets were obtained at baseline (n = 21) and at month.

Effects of DWJM on UCB CD34+-derived cell subsets.

Real Time Immunophenotyping of Leukocyte Subsets Early after Double Cord Blood Transplantation Predicts Graft Function Jianqiang Li, Ian Nicoud, Joseph.

A flow cytometry–based diagnosis of eosinophilic esophagitis

Development, Simulation and Optimization of Oncology Laboratory Sample Processing Automation Emily Salmon Advisor: Norman Purvis, PhD, Esoterix.

ALT-803 stimulates proliferation and activation of human NK cells and T cells in vitro. ALT-803 stimulates proliferation and activation of human NK cells.

B1b Lymphocytes Confer T Cell-Independent Long-Lasting Immunity

PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate distinct mechanisms of regulation. PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate.

Flow cytometric gating strategy used to identify CD69 surface expression on CD4 (left paired columns) and CD8 (right paired columns) T lymphocytes. Flow.

Mice with a B cell–specific deletion of Ets1 do not have increased CD4+ T cell activation. Mice with a B cell–specific deletion of Ets1 do not have increased.

Generation of Functional Thymocytes in the Human Adult

Presentation transcript:

External Quality Assessment: Choosing an EQA program University of Nairobi/University of Washington Peadiatrics Research Laboratory John Gatimu University of Nairobi/University of Washington Research Laboratory

External Quality Assessment (EQA) Labs need to evaluate the various EQA programs to identify one that best suits SOPs Study protocols EQA specimens should be analyzed the same way patient samples are analyzed Applicable to a wide range of clinical laboratory assays

Analyzing Flow Cytometry Samples Using FACS Calibur samples can be gated automatically or manually In general, good correlation between automatic and manual gating Differences indicate a problem In our lab only a small number of samples require manual gating

Objective To identify an EQA program that best suits our laboratory’s SOPs and study protocols

Objectives cont and Methodology Measured objectives by: 1. Evaluating percent change in absolute CD3, CD4 and CD8 cell count between gating methods 2. Analyzing statistical differences in SD intervals (SDI) from automated vs manual gating using a paired t-test 3. Obtaining failure rate from each company Compared 10 EQA specimens from 2 companies Used FACS Calibur SOPs

Patient samples: Similar Counts Between Gating Method Automated gated Manual gated 929 931 440 441 457 462

EQA Samples: Differences Between Gating Method Automated gated Manual gated 1121 1521 757 718 733 344

Results: Manual Gating results in increased absolute counts

Results: Using True Mean Derived from Company Company A : Lymphocyte Subset Auto gated Manual gated P value CD4 0.32 SDI 0.10 SDI 0.0095 CD3 3.10 SDI -0.417 SDI 0.0002 CD8 4.98 SDI -0.66 SDI 0.0001 Company B Lymphocyte Subset Auto gated Manual gated P value CD4 -0.09 SDI 0.06 SDI 0.6529 CD3 -0.22 SDI -0.31 SDI 0.0200 CD8 0.66 SDI 0.12 SDI 0.0005

Results: Percent Failure rate Percent of tests > 2SD of true mean Automated gating Lymphocyte Subset Company A Company B P value CD4 0% 10% 0.3506 CD3 70% 0.0051 CD8 90% 0.0000

Summary and Conclusion Differences were noted for: Percent change in absolute CD3, CD4 and CD8 counts after manual gating SDIs Failure rate Labs should evaluate specimens from various EQA programs in order to identify one that best suits the labs SOPs and study protocols This process is applicable to other clinical laboratory tests

Barbara Lohman-Payne Judd Walson CFAR Tanja Hart UW Amy Liu KEMRI Acknowledgements Barbara Lohman-Payne Judd Walson Tanja Hart Amy Liu Michele Merkel Nick Bright Anna Wambugu CFAR UW KEMRI UoN BD