Ovine secondary follicles vitrified out the ovarian tissue grow and develop in vitro better than those vitrified into the ovarian fragments Franciele.

Slides:

Advertisements

Similar presentations

1 Copyright © 2010, Elsevier Inc. All rights Reserved Fig 4.1 Chapter 4.

Advertisements

Nucleation and Transport Organize Microtubules in Metaphase Spindles Jan Brugués, Valeria Nuzzo, Eric Mazur, Daniel J. Needleman Cell Volume 149, Issue.

Human Brown Adipose Tissue Sven Enerbäck Cell Metabolism Volume 11, Issue 4, Pages (April 2010) DOI: /j.cmet Copyright © 2010.

ABO-incompatible kidney transplantation Kota Takahashi, Kazuhide Saito Transplantation Reviews Volume 27, Issue 1, Pages 1-8 (January 2013) DOI: /j.trre

Anti-HIV drugs: 25 compounds approved within 25 years after the discovery of HIV Erik De Clercq International Journal of Antimicrobial Agents Volume 33,

Recovery of normal testicular temperature after scrotal heat stress in rams assessed by infrared thermography and its effects on seminal characteristics.

DOI: /j.theriogenology

Expression of PLIN2 and PLIN3 during oocyte maturation and early embryo development in cattle Danuta Sastre, Nathália Nogueira da Costa, André Luiz Alves.

The functional effect of soybean extract and isolated isoflavone on myocardial infarction and ventricular dysfunction Ana C. Miguez, Julio C. Francisco,

Follicular right shift: Xenografting queens' ovarian tissue into severe combined imunnodeficiency mice and its responses to exogenous gonadotropin Fernanda.

Endometrial prostaglandin synthases, ovarian steroids, and oxytocin receptors in mares with oxytocin-induced luteal maintenance Maria R. Rebordão, António.

M.M. Wanke, M.V. Delpino, P.C. Baldi Theriogenology

DOI: /j.theriogenology

Accelerated follicle growth during the culture of isolated caprine preantral follicles is detrimental to follicular survival and oocyte meiotic resumption

Morphological and molecular differences in corpus luteum of pregnant sows from divergent genetic groups Karine A. Costa, Walmir da Silva, Renata Veroneze,

Use of computer-assisted sperm analysis and flow cytometry to detect seasonal variations of bovine semen quality E. Malama, Y. Zeron, F. Janett, M. Siuda,

Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human.

Association of luteal blood flow with follicular size, serum estrogen and progesterone concentrations, and the inducibility of luteolysis by PGF2α in.

UPLC-MS/MS determination in blood of a mixed-drug fatal intoxication: A case report Paula Proença, João Miguel Franco, Carla Mustra, Carla Monteiro, Joana.

Trimethylation of histone 3 at lysine 4 in cryopreserved bovine embryos produced in vivo with sexed semen Mirela B. Souza Cáceres, Wilian A. Leite da.

Poliana M. Duarte, Marcelo H. Napimoga, Ellen C. Fagnani, Vanessa R

Impact of temperature and time storage on the microbial detection of oral samples by Checkerboard DNA–DNA hybridization method Cássio do Nascimento,

BIOLOGICAL PATHWAYS FOUND IN COMMON BETWEEN ALZHEIMER’S DISEASE AND MAJOR DEPRESSION: A STUDY ON MICRORNA EXPRESSION IN A SYSTEMATIC REVIEW Ana Paula.

Direct vitamin D3 actions on rhesus macaque follicles in three-dimensional culture: assessment of follicle survival, growth, steroid, and antimüllerian.

Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification Gary D. Smith, Ph.D., Paulo C. Serafini,

Developmental programming: prenatal testosterone excess disrupts anti-Müllerian hormone expression in preantral and antral follicles Almudena Veiga-Lopez,

Acquisition and loss of oocyte meiotic and developmental competence during in vitro antral follicle growth in mouse Ingrid Segers, M.Sc., Tom Adriaenssens,

Cigarette smoke condensate exposure delays follicular development and function in a stage-dependent manner Jean Clair Sadeu, M.D., Ph.D., Warren G. Foster,

Developmental capacity of in vitro–matured human oocytes retrieved from polycystic ovary syndrome ovaries containing no follicles larger than 6 mm Luis.

IVM and gene expression of sheep cumulus–oocyte complexes following different methods of vitrification Bita Ebrahimi, Mojtaba Rezazadeh Valojerdi, Poopak.

Successful slush nitrogen vitrification of human ovarian tissue

The impact of culture conditions on early follicle recruitment and growth from human ovarian cortex biopsies in vitro Jana Liebenthron, M.Sc., Maria.

Vitrification and xenografting of human ovarian tissue

Androstenedione induces abnormalities in morphology and function of developing oocytes, which impairs oocyte meiotic competence Wataru Tarumi, M.Sc.,

Silke Klocke, Claudia Tappehorn, Georg Griesinger

Hung-Ching Liu, Ph.D., Zhiying He, M.S., Zev Rosenwaks, M.D.

In vitro blastocyst formation of human oocytes obtained from unstimulated and stimulated cycles after vitrification at various maturational stages Hyung.

Erasmia Kiapekou, M. D. , Evangelia Zapanti, M. D

Improvement of in vitro culture of mouse cumulus–oocyte complexes using PDE3- inhibitor followed by meiosis induction with epiregulin Sergio Romero, M.Sc.,

Evidence that obesity alters the quality of oocytes and embryos

Shi Ying Jin, Ph. D. , Lei Lei, Ph. D. , Ariella Shikanov, Ph. D

Pup birth from mouse oocytes in preantral follicles derived from vitrified and warmed ovaries followed by in vitro growth, in vitro maturation, and in.

Morphologic, ultrastructural, and biochemical identification of apoptosis in vitrified- warmed mouse ovarian tissue Tahere Mazoochi, M.Sc., Mojdeh Salehnia,

Survival and growth of human preantral follicles after cryopreservation of ovarian tissue, follicle isolation and short-term xenografting Fernanda Paulini,

Effect of long-term exposure at suprazero temperatures on activity and viability of human ovarian cortex Evgenia Isachenko, Ph.D., Vladimir Isachenko,

Clinical Assessment of Recombinant Human Follicle-Stimulating Hormone in Stimulating Ovarian Follicular Development Before In Vitro Fertilization fn1

Platelet-rich plasma promotes the development of isolated human primordial and primary follicles to the preantral stage Laleh Hosseini, Abolfazl Shirazi,

Effects of follicular phase and oocyte–cumulus complexes quality on the protein profile and in vitro oocyte meiosis competence in Cebus apella Sheyla.

Direct comparative analysis of conventional and directional freezing for the cryopreservation of whole ovaries Sara Maffei, M.Sc., Maike Hanenberg, M.Sc.,

Patricia Miyuki Tsuribe, Ph. D. , Carlos Alberto Monte Gobbo, M. D

Gene injections of vascular endothelial growth factor and growth differentiation factor-9 stimulate ovarian follicular development in immature female.

Epigenetic disturbances in in vitro cultured gametes and embryos: implications for human assisted reproduction Nady el Hajj, Ph.D., Thomas Haaf, M.D.

Antibiotic De-Escalation

Immunohistochemical localization of growth factors after cryopreservation and 3 weeks' xenotransplantation of human ovarian tissue Anu David, M.Sc.,

Timed analysis of the nuclear maturation of oocytes in early preantral mouse follicle culture supplemented with recombinant gonadotropin Rita G Cortvrindt,

Ana Cobo, Ph. D. , Juan A. García-Velasco, M. D. , Aila Coello, Ph. D

Organization news Archives of Physical Medicine and Rehabilitation

Fertility and Sterility

Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human.

Exogenous androstenedione induces formation of follicular cysts and premature luteinization of granulosa cells in the ovary Yuki Okutsu, M.D., Masanori.

Novel dynamic culture system to support initiation of primordial follicle growth in prepubertal mouse ovaries Katharina Winkler-Crepaz, M.D., Verena.

Fertility and Sterility

Derivation of developmentally competent oocytes by in vitro culture of preantral follicles retrieved from aged mice Jung Kyu Choi, B.Sc., Jong Il Ahn,

Comparison of urinary and recombinant follicle-stimulating hormone in in vitro growth, maturation, and fertilization of mouse preantral follicles Giannina.

Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in.

Ultrastructure of follicles after vitrification of mouse ovarian tissue Mojdeh Salehnia, Ph.D., Esmat Abbasian Moghadam, Mojtaba Rezazadeh Velojerdi,

Allotransplantation of cryopreserved prepubertal mouse ovaries restored puberty and fertility without affecting methylation profile of Snrpn-DMR Hong-Yan.

Dimethyl sulfoxide perfusion in caprine ovarian tissue and its relationship with follicular viability after cryopreservation Valesca B. Luz, D.V.M.,

Ovarian follicular volume and follicular surface area are better indicators of follicular growth and maturation, respectively, than is follicular diameter

Histologic and ultrastructural features of cryopreserved ovine ovarian tissue: deleterious effect of 1,2-propanediol applying different thawing protocols

Presentation transcript:

Ovine secondary follicles vitrified out the ovarian tissue grow and develop in vitro better than those vitrified into the ovarian fragments Franciele Osmarini Lunardi, Francisco Leo Nascimento de Aguiar, Ana Beatriz Graça Duarte, Valdevane Rocha Araújo, Laritza Ferreira de Lima, Naiza Arcângela Ribeiro de Sá, Hudson Henrique Vieira Correia, Sheyla Farhayldes Souza Domingues, Cláudio Cabral Campello, Johan Smitz, José Ricardo de Figueiredo, Ana Paula Ribeiro Rodrigues Theriogenology Volume 85, Issue 7, Pages 1203-1210 (April 2016) DOI: 10.1016/j.theriogenology.2015.10.043 Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 1 Percentage of isolated morphologically normal sheep preantral follicles without vitrification (control, n = 50) and after vitrification of isolated follicles (follicle-vit, n = 40) or ovarian tissue (tissue-vit, n = 64) at Days 0, 6, 12, and 18 of IVC. ABDifferent uppercase letters indicate statistically significant differences among groups (P < 0.05) within the same day. abDifferent lowercase letters indicate statistically significant differences among days of culture (P < 0.05) within the same group. Theriogenology 2016 85, 1203-1210DOI: (10.1016/j.theriogenology.2015.10.043) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 2 Normal sheep secondary follicle before (Day 0) IVC (A) or cultured for 6 days (B), 12 days (C), or 18 days (D) in follicle-vit group. Note beginning of antrum formation at Day 6 of IVC. (E) Percentage of antrum formation of sheep preantral follicles without vitrification (control, n = 39) and after vitrification of isolated follicles follicle-vit, n = 39) or ovarian tissue (tissue-vit, n = 60) at Days 6, 12, and 18 of IVC. ABDifferent uppercase letters indicate statistically significant differences among groups (P < 0.05) within the same day. abDifferent lowercase letters indicate statistically significant differences among days of culture (P < 0.05) within the same group. Theriogenology 2016 85, 1203-1210DOI: (10.1016/j.theriogenology.2015.10.043) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 3 Follicular diameter (μm; mean ± standard error of the mean) of sheep preantral follicles without vitrification (control, n = 21) and after vitrification of isolated follicles (follicle-vit, n = 38) or ovarian tissue (tissue-vit, n = 44) at Days 0, 6, 12, and 18 of IVC. AThe uppercase letter indicates statistically significant differences among groups (P < 0.05) within the same day. abcDifferent lowercase letters indicate statistically significant differences among days of culture (P < 0.05) within the same group. Theriogenology 2016 85, 1203-1210DOI: (10.1016/j.theriogenology.2015.10.043) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 4 (A) Daily growth rate (μm/day; mean ± standard error of the mean) of sheep preantral follicles without vitrification (control, n = 21) and after vitrification of isolated follicles (follicle-vit, n = 38) or ovarian tissue (tissue-vit, n = 44) after 18 days of IVC and (B) Percentage of sheep preantral follicles with decreased diameter from control (n = 18), follicle-vit (n = 1), and tissue-vit (n = 16) after 18 days of culture. ABCDifferent uppercase letters indicate statistically significant differences among groups (P < 0.05). Theriogenology 2016 85, 1203-1210DOI: (10.1016/j.theriogenology.2015.10.043) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 5 Viable oocyte in metaphase I after IVM from follicle-vit group after IVC for 18 days. Brightfield (A), Calcein-AM (B), and Hoechst 33342 (C). Theriogenology 2016 85, 1203-1210DOI: (10.1016/j.theriogenology.2015.10.043) Copyright © 2016 Elsevier Inc. Terms and Conditions