From: Phosphatase-Mediated Crosstalk Control of ERK and p38 MAPK Signaling in Corneal Epithelial Cells Invest. Ophthalmol. Vis. Sci.. 2006;47(12):5267-5275. doi:10.1167/iovs.06-0642 Figure Legend: Selectivity of siRNA MKP-1 to suppress MKP-1 protein expression. Nontransfected RCECs were exposed to 10 ng/mL EGF for 60 minutes. RCECs were transfected with physiologically irrelevant commercially provided negative control MKP-1 siRNA (neg con siRNA), its transfection reagent (Rea), or siRNA MKP-1 for 72 hours, followed by 24-hour serum starvation. Subsequently, cells were stimulated with 10 ng/mL EGF for 60 minutes. Western blot analysis was then performed using an anti–MKP-1 antibody. Equal loading of proteins was confirmed by reprobing the same blot with anti–IgG antibody. Data represent the mean ± SEM of results in triplicate experiments. Date of download: 12/26/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.