Microemulsion In Drug Delivery Systems 9/12/2018 An-Najah National University Chemical Engineering Department Microemulsion In Drug Delivery Systems Prepared by: Alaa Badran Ibtihaj Awwad Hana’a Tareq Hadeel Suliman Superior : Dr. Husni Odeh Submitted to Chemical Engineering Department Second Semester, 2010-2011 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Outline Objectives. Microemulsion . Advantage and disadvantage of microemulsion. Solubility and Bioavailability. Earlier study of microemulsion in the drug. Materials. Experimental work. Results and discussion. Conclusion. Recommendation. 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

9/12/2018 The objectives This project aim to design and develop of microemulsion drug delivery system . Improved the solubility and bioavailability of curcumin drug by using microemulsion. 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Microemulsions Microemulsion are liquid dispersions of water and oil that are made homogenous, transparent and thermodynamically stable by the addition of relatively large amounts of surfactant and co-surfactant and having diameter of the droplets in the range of 100 – 1000 A (10 – 100 nm). 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

The advantages of Microemulsion Microemulsions are thermodynamically stable system and the stability allows self-emulsification of the system whose properties are not dependent on the process followed. Microemulsion have low viscosity compared to other emulsions. 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Microemulsion can improve the efficacy of a drug, allowing the total dose to be reduced and thus minimizing side effects. microemulsion are easy to prepare and require no significant energy contribution during preparation.  9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

The disadvantage of Microemsultion Use of a large concentration of surfactant and co-surfactant necessary for stabilizing the nano-droplets. The surfactant must be nontoxic for using pharmaceutical applications . 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Microemulsion stability is influenced by environmental parameters such as temperature and pH.  These parameters change upon microemulsion delivery to patients. 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

BIOAVALABILITY Bioavailability of a drug is largely determined by the properties of the dosage form. The therapeutic index : ratio of the minimum toxic concentration to the median effective concentration. Low bioavailability is most common with oral dosage forms of poorly water-soluble, slowly absorbed drugs. 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

SOLUBILITY Solubility : the amount of a substance that dissolves in a given volume of solvent at a specified temperature. Poor aqueous solubility . High lipophilicity. 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Earlier Studies On Microemulsion In The drug Cyclovir Labrasol (surfactant) 32% Plurol Oleique ((co-surfactant 8% Labrafac (oil) 10% Water 50% Bioavailability increases (12.78 times) 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Valsartan Tween 80 (surfactant) 45% PEG 400 ((co-surfactant Capmul MCM (oil) 10% The release increased more than 90% when compared with marketed formulation 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Glyburide (GLY) Tween-20(sur). Capryol 90 (oil) Water Transcutol P (co-sur) Capryol 90 (oil) Water 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Material The drug: curcmin (anticancer). The surfactant and co-surfactant: Tween20 (Polysorbate), Spans80(Sorbitan monooleate) Oil: Jojoba with clove (95%/5%) and clove oil(~100%) 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Microemulsion systems System no.1 Tween 20, Span 80 (Sur. Mix) Jojoba oil(95%), clove oil (5%) System no. 2 Tween 20, span80 (Sur. Mix.) Clove oil ≃100% 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Microemulsion Preparation The Procedures Curcumin Preparation Microemulsion Preparation Solubility Test 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Addition af surfactant/ co-surfactant Addition of oils Addition of water. Shaking. Microemulsion Formulation 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Table(1): Initial concentration values of oil and Sur. Mix Table(1): Initial concentration values of oil and Sur. Mix. For the two systems Amount of oil 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 Amount of Sur. Mix 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Drug solubility experiments Drug solubility experiments were carried out based on the previous microemulsion experiments. It was experienced that for: First system jojoba oil and clove oil: surfactant/co-surfactant, oil, water(3.01%,27.07%,69.9%). surfactant/co-surfactant, oil, water (6%,24.28%,69.72%). surfactant/co-surfactant, oil, water(9.02%,21.11%,69.87%) . 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Second system clove oil : surfactant/cosurfactant,oil, water (3%, 27.14%, 69,86%). surfactant/cosurfactant,oil, water(6.02%,24.06%,69.92%). surfactant/cosurfactant,oil, water (9.01%,21.15%,69.84%) . 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Results and Discussion Due to the addition of water the shape and color of emulsions were changed.(3, 2, 1 phases) 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Results and discussion 9/12/2018 Figure(1): Triangle Phase Diagram of oil , Sur. Mix. And Water

The highest solubility of curcumin 5mg/ml is found at (20%,10%,70%): (S mix./oil/water) microemulsion system. 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Conclusion Microemulsion is excellent candidate as potential drug delivery system. The microemulsion system occurred at (20% Smix ,10% oil ,70% water). The highest drug solubility of curcumine 5mg/ml is found at (20%/10%/70%) : (Smix/oil/water) microemulsion system. 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Recommendations 1. Microemulsion method is one of the best method of drug delivery to increase bioavailability of drug. 2. Deep study must be done for each material used in preparation of ME. 3. Careful dealing with components while adding them to the system. 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

4. Use pre-calibrated devices for measuring amounts of components. 5. Use suitable shaking device that offer gentle mixing for several tubes that save time and effort. 6. Use Pure Water in Preparation of ME 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system

Thank You 9/12/2018 Chemical Engineering Department-Graduation Project-Microemulsion Drug Delivery system