Copyright © 2006 American Medical Association. All rights reserved. From: Hyaluronan-CD44 Promotes Phospholipase C–Mediated Ca2+ Signaling and Cisplatin Resistance in Head and Neck Cancer Arch Otolaryngol Head Neck Surg. 2006;132(1):19-24. doi:10.1001/archotol.132.1.19 Figure Legend: Proposed model of a hyaluronan (HA)-CD44–mediated phospholipase C (PLC)/Ca2+ signaling pathway in head and neck squamous cell carcinoma. PIP2 indicates phosphatidylinositol-4,5-bisphosphate; P, phosphorylation; DG, diacylglycerol; IP3, inositol-1,4,5-triphosphate; and IP3R, inositol-1,4,5-triphosphate receptor. Date of download: 12/27/2017 Copyright © 2006 American Medical Association. All rights reserved.

Copyright © 2006 American Medical Association. All rights reserved. From: Hyaluronan-CD44 Promotes Phospholipase C–Mediated Ca2+ Signaling and Cisplatin Resistance in Head and Neck Cancer Arch Otolaryngol Head Neck Surg. 2006;132(1):19-24. doi:10.1001/archotol.132.1.19 Figure Legend: Immunoblot of cell lysates from HSC-3 (lane 1) and SCC-4 (lane 2), probed with anti-CD44 antibody. Bands are seen corresponding to standard CD44 (85-90 kDa) and several higher-molecular-weight CD44 variants. Date of download: 12/27/2017 Copyright © 2006 American Medical Association. All rights reserved.

Copyright © 2006 American Medical Association. All rights reserved. From: Hyaluronan-CD44 Promotes Phospholipase C–Mediated Ca2+ Signaling and Cisplatin Resistance in Head and Neck Cancer Arch Otolaryngol Head Neck Surg. 2006;132(1):19-24. doi:10.1001/archotol.132.1.19 Figure Legend: HSC-3 cells were grown in serum-free media in increasing concentrations of cisplatin in the presence or absence of hyaluronan (HA) (50 μg/mL). Graph displays cell growth as a percentage of controls. Dashed line indicates 50% inhibitory concentrations (IC50). Error bars represent calculated standard deviations. Date of download: 12/27/2017 Copyright © 2006 American Medical Association. All rights reserved.

Copyright © 2006 American Medical Association. All rights reserved. From: Hyaluronan-CD44 Promotes Phospholipase C–Mediated Ca2+ Signaling and Cisplatin Resistance in Head and Neck Cancer Arch Otolaryngol Head Neck Surg. 2006;132(1):19-24. doi:10.1001/archotol.132.1.19 Figure Legend: Fluorescence spectrophotometry was used to determine intracellular Ca2+ concentration over time. After HSC-3 cells had been incubated with Fura-2/AM (CalBiochem; EMD Biosciences, San Diego, Calif), they were treated with various agents: hyaluronan (HA) treatment (50 μg/mL) (A), anti-CD44 antibody followed by HA treatment (50 μg/mL) (B), U73122 (5μM) followed by HA treatment (50 μg/mL) (C), and 2-aminoethoxydiphenyl borate (2-APB) (50μM) followed by HA treatment (50 μg/mL) (D). Similar results were obtained with SCC-4 cells (data not shown). Date of download: 12/27/2017 Copyright © 2006 American Medical Association. All rights reserved.

Copyright © 2006 American Medical Association. All rights reserved. From: Hyaluronan-CD44 Promotes Phospholipase C–Mediated Ca2+ Signaling and Cisplatin Resistance in Head and Neck Cancer Arch Otolaryngol Head Neck Surg. 2006;132(1):19-24. doi:10.1001/archotol.132.1.19 Figure Legend: HSC-3 cells grown in serum-free media were treated with cisplatin in the presence or absence of hyaluronan (HA) (50 μg/mL) plus U73122 (5μM). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed. Inset, U73122 alone had little effect on growth proliferation at 5μM. Error bars represent calculated standard deviations. Date of download: 12/27/2017 Copyright © 2006 American Medical Association. All rights reserved.

Copyright © 2006 American Medical Association. All rights reserved. From: Hyaluronan-CD44 Promotes Phospholipase C–Mediated Ca2+ Signaling and Cisplatin Resistance in Head and Neck Cancer Arch Otolaryngol Head Neck Surg. 2006;132(1):19-24. doi:10.1001/archotol.132.1.19 Figure Legend: HSC-3 cells grown in serum-free media were treated with cisplatin in the presence or absence of hyaluronan (HA) (50 μg/mL) plus 2-aminoethoxydiphenyl borate (2-APB) (50μM). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed. Inset, 2-APB alone had little effect on growth proliferation at 50μM. Error bars represent calculated standard deviations. Date of download: 12/27/2017 Copyright © 2006 American Medical Association. All rights reserved.