From: The Effect of Vapor of Propylene Glycol on Rats

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From: The Effect of Vapor of Propylene Glycol on Rats Figure 1 Differences in amplitude between the baseline and test data for the ASR. Subject rats were presented with sample or control (purified water) stimuli in counterbalanced order between the baseline trial and the test trial during 2 consecutive experimental days. Samples presented were PG at doses of 1 × 10<sup>−2</sup> M (n = 10, A), 1 × 10<sup>−4</sup> M (n = 14, B), and 1 × 10<sup>−6</sup> M (n = 10, C), and 1,3-PD at a dose of 1 × 10<sup>−4</sup> M (n = 14, D). Each bar represents the mean ± standard error; *P < 0.05 versus control (paired t-test). From: The Effect of Vapor of Propylene Glycol on Rats Chem Senses. 2010;35(3):221-228. doi:10.1093/chemse/bjp104 Chem Senses | © The Author 2010. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

From: The Effect of Vapor of Propylene Glycol on Rats Figure 2 The investigation time for 3 consecutive water stimuli (Water 1–3) and the following PG stimuli: 1 M (n = 8), 1 × 10<sup>−2</sup> M (n = 8), and 1 × 10<sup>−4</sup> M (n = 8). Each bar represents the mean ± standard error; **P < 0.01 versus the third water stimulus (paired t-test). From: The Effect of Vapor of Propylene Glycol on Rats Chem Senses. 2010;35(3):221-228. doi:10.1093/chemse/bjp104 Chem Senses | © The Author 2010. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

From: The Effect of Vapor of Propylene Glycol on Rats Figure 3 Ca<sup>2+</sup> responses induced by PG in VNs. (A) A confocal fluorescence image acquired at rest (pseudocolor scale); (B) a confocal image acquired just after application of PG to the bath; (C) an image made by subtracting image A from image B, indicating that a limited distribution of VNs respond to PG (arrows); and (D) changes in fluorescence intensity recorded from a VN marked by a gray arrow in image C. Bars below traces indicate the periods in which the indicated stimuli were applied. From: The Effect of Vapor of Propylene Glycol on Rats Chem Senses. 2010;35(3):221-228. doi:10.1093/chemse/bjp104 Chem Senses | © The Author 2010. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

From: The Effect of Vapor of Propylene Glycol on Rats Figure 4 Changes in fluorescence intensity recorded from VNs in response to increasing concentrations of PG (A) and 1,3-PD (B). Bars below traces indicate the periods in which the indicated stimuli were applied. From: The Effect of Vapor of Propylene Glycol on Rats Chem Senses. 2010;35(3):221-228. doi:10.1093/chemse/bjp104 Chem Senses | © The Author 2010. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

From: The Effect of Vapor of Propylene Glycol on Rats Figure 5 Dose dependency of stimulus-induced Ca<sup>2+</sup> responses of VNs that recognized either PG (closed circle) or 1,3-PD (open circle). Relative magnitudes of the responses (rR) to PG and 1,3-PD were plotted as a function of chemical concentration. The magnitude of the response to 1 × 10<sup>−4</sup> M PG was taken as unity. Each point is the mean ± standard error obtained from 6 neurons for PG and 8 neurons for 1,3-PD; **P < 0.01 versus relative response to 1,3-PD at the corresponding concentration (2-way repeated measures analysis of variance followed by Student's t-test). Note the difference in detection thresholds. From: The Effect of Vapor of Propylene Glycol on Rats Chem Senses. 2010;35(3):221-228. doi:10.1093/chemse/bjp104 Chem Senses | © The Author 2010. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org