Mod1 Day6 Thursday Sept 25th

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Presentation transcript:

Mod1 Day6 Thursday Sept 25th Tissue Culture Mod1 Day6 Thursday Sept 25th

Today’s Lab: part 1 Restriction Map While your gel runs you’ll discuss RAD51 paper

Today’s Lab: part 2 TC for fun

Grow mammalian vs bacterial cells For both need: For bacteria: Tryptone, yeast extract, salt +/- antibiotic, agar For MES cells: DMEM, calf serum, glutamine, penn/strep

Figuring out how many cells you have

Things to do for next time Submit lab report by email before the start of lecture Read Mod1 Day7 + do calculations for experiment Answer one hypothetical question about doubling times + cell #