Association of Platelet-Derived Growth Factor Receptor α Mutations with Gastric Primary Site and Epithelioid or Mixed Cell Morphology in Gastrointestinal.

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Association of Platelet-Derived Growth Factor Receptor α Mutations with Gastric Primary Site and Epithelioid or Mixed Cell Morphology in Gastrointestinal Stromal Tumors Eva Wardelmann, Aksana Hrychyk, Sabine Merkelbach-Bruse, Katharina Pauls, Jennifer Goldstein, Peter Hohenberger, Inge Losen, Christoph Manegold, Reinhard Büttner, Torsten Pietsch The Journal of Molecular Diagnostics Volume 6, Issue 3, Pages 197-204 (August 2004) DOI: 10.1016/S1525-1578(10)60510-7 Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 SSCP and sequence analysis in exon 18 of PDGFRα. A: GIST 47: Point mutation (T -> A) leading to an amino acid exchange from asparagine to valine; DNA fragment with altered mobility marked by an arrow. B: GIST 55: Deletion of 9 bp leading to an amino acid change in codon 842 from asparagine to alanine and to a loss of codons 843 to 845 (isoleucine, methionine, and histidine; DNA fragment with altered mobility marked by an arrow). The Journal of Molecular Diagnostics 2004 6, 197-204DOI: (10.1016/S1525-1578(10)60510-7) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Histomorphology and expression of KIT and PDGFRα receptors in two PDGFRα-mutated GISTs: Both tumors exhibited a mixed phenotype (GIST 55 in A, GIST 47 in B; H&E). GIST 55 showed a weak KIT receptor expression (C) and a strong membranous and dot-like cytoplasmatic PDGFRα receptor expression (E). GIST 47 showed a strong, but only focal KIT receptor expression (D) and a strong membranous and cytoplasmatic PDGFRα receptor expression (F). (Original magnification, ×400, A–F). The Journal of Molecular Diagnostics 2004 6, 197-204DOI: (10.1016/S1525-1578(10)60510-7) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions