Figure 1. Equal cycle RT-PCR of selected genes up-regulated in eutopic human endometrium during the window of implantation, from women without (N) and.

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Presentation transcript:

Figure 1. Equal cycle RT-PCR of selected genes up-regulated in eutopic human endometrium during the window of implantation, from women without (N) and with (D) endometriosis. Specific primer pairs used are shown in Table 1. Appropriate size bands are depicted for the housekeeping gene GAPDH (lane 1), as well as for the up-regulated genes: semaphorin E (lane 2), collagen α-2 type I (lane 3), and ankyrin G (lane 4). Two samples from women without and two from women with endometriosis were used for this study; representative results are shown. From: Expression Profiling of Endometrium from Women with Endometriosis Reveals Candidate Genes for Disease-Based Implantation Failure and Infertility Endocrinology. 2003;144(7):2870-2881. doi:10.1210/en.2003-0043 Endocrinology | Copyright © 2003 by The Endocrine Society

Figure 2. Equal cycle RT-PCR of selected genes down-regulated in eutopic human endometrium during the window of implantation, from women without (N) and with (D) endometriosis. Specific primer pairs used are shown in Table 1. Appropriate size bands are depicted for: integrin α2 subunit (lanes 1), platelet-derived endothelial cell growth factor (PD-EGF) (lanes 2), GlcNAC (lanes 3), B61/Ephrin (lanes 4), and glycodelin (lanes 5). Two samples from women without and two from women with endometriosis were used for this study; representative results are shown. From: Expression Profiling of Endometrium from Women with Endometriosis Reveals Candidate Genes for Disease-Based Implantation Failure and Infertility Endocrinology. 2003;144(7):2870-2881. doi:10.1210/en.2003-0043 Endocrinology | Copyright © 2003 by The Endocrine Society

Figure 3. Northern blot analyses demonstrating: (A) up-regulation of collagen α-2 type I, (B) down-regulation of GlcNAC, glycodelin, integrin 2 α-subunit and B61, in eutopic human endometrium during the window of implantation, from women without (a) or with (b) endometriosis. C, Dot-blot analysis for up-regulation of BSEP. Three samples from women without and two from women with endometriosis were used and representative results are shown. GAPDH hybridization densities of corresponding lanes are also shown for comparison (see also Table 4). From: Expression Profiling of Endometrium from Women with Endometriosis Reveals Candidate Genes for Disease-Based Implantation Failure and Infertility Endocrinology. 2003;144(7):2870-2881. doi:10.1210/en.2003-0043 Endocrinology | Copyright © 2003 by The Endocrine Society