Invest. Ophthalmol. Vis. Sci ;51(10): doi: /iovs Figure Legend:

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From: Prevention of Endotoxin-Induced Uveitis in Rats by Plant Sterol Guggulsterone Invest. Ophthalmol. Vis. Sci.. 2010;51(10):5105-5113. doi:10.1167/iovs.09-4873 Figure Legend: Gugglesterone prevents inflammatory changes in HNPCECs stimulated with LPS. (A) Growth-arrested HNPCECs pretreated with or without gugglesterone (40 μM) were incubated with 1 μg/mL LPS for 24 hours. Expression of Cox-2, iNOS, AR and phospho-p65 from cell lysates was determined by Western blot analysis using specific antibodies. (B) Growth-arrested HNPCECs pretreated with or without gugglesterone (40 μM) were incubated with 1 μg/mL LPS for 0 to 120 minutes. PI3K and Akt from total cell lysates were determined by Western blot analysis using respective phosphospecific antibodies. (C) Levels of TNF-α in the culture media were determined with ELISA kit. Data are expressed as the mean ± SD (n = 6). *P < 0.001 versus the control group. **P < 0.001 versus the LPS group. Gugg, guggulsterone. Date of download: 12/28/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.