Abhyudai Singh absingh@udel.edu Systems-level modeling of genetic circuits Gene A Protein A Protein B Gene B Abhyudai Singh absingh@udel.edu

Genetic circuits exhibit stochastic behavior thermal noise; low-copy numbers (shot noise) Suter et al. Science 2011 How do circuits robustly perform biological function? Mechanisms for buffering stochastic fluctuations? Can fluctuations reveal information about underlying circuits?

Building tools for stochastic modeling of genetic circuits Protein B Gene B Dynamical Systems; Control Theory; Stochastic Hybrid Systems; Queuing Theory; Statistical Physics……. Protein A Gene A Data Model #1 Model #2 Freqeuncy Protein level/cell

Cell-fate decision in the HIV model system HIV Infection Lysis Latent Tat Rev GFP The title of my talk is … This talk will be somewhat different than the paper that appears in the proceedings because I’ll be somewhat tutorial and concentrate much more on the motivation and background behind this work and only at the end I’ll tell you about a new result that we obtained recently and that you’ll find in the proceedings paper This seemed more appropriate for a workshop like this. If some of you in the audience are very familiar with systems biology, I apologize in advance because in the talk I’ll go through very basic material.