Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells Qianfa Long 1, 2 ;Qiang Luo a ;Kai Wang 3 ;Adrian Bates 2, 4 ;Ashok K. Shetty 2, 4 ; 1 Department of Neurosurgery, Xi#cod#x02019;an Central Hospital, Xi#cod#x02019;an Jiao Tong University School of Medicine, Xi#cod#x02019;an 710003, China ; 2 Institute for Regenerative Medicine, Texas A#cod#x00026;M Health Science Center College of Medicine at Scott #cod#x00026; White, Temple and College Station, Texas, 76502, USA ; 3 Department of Neurosurgery, Qingdao 401 Hospital of PLA, Qingdao 266071, China ; 4 Research Service, Olin E. Teague Veterans#cod#x02019; Medical Center, CTVHCS, Temple, Texas, USA ; Figure 5. Immunofluorescence showing GABAergic neuron-like differentiation from Mash1 overexpressed BMSCs After Mash1+ BMSCs were induced by cocktail conditioned medium, immunofluorescence showed that differentiated cells expressed neuronal cytoskeleton marker NF-H C , white arrow, mature neuronal marker NeuN H , white arrow, and specific neuronal marker #cod#x003B2;-3 tubulin M , white arrow, in addition to GABA-ergic cell marker GAD67 D, I and N , white arrow. Note that, many GAD67 positive cells co-localize with NF-H A E , white arrow, NeuN F J , white arrow, and #cod#x003B2;-3 tubulin K O , white arrow. B, G and L illustrate DAPI stained nuclei. Bar A, F and K = 50 #cod#x003BC;m, bar E, J and O = 20 #cod#x003BC;m. Statistical analysis shows the percentage of GABA-ergic neuron-like differentiation in genetically engineered BMSCs P . Statistical differences referred to Lv-con-BMSCs, #cod#x0002A; P = 1.9036E-05, #cod#x0003C; 0.01; #cod#x0002A;#cod#x0002A; P = 3.61045E-05, #cod#x0003C; 0.01; #cod#x0002A;#cod#x0002A;#cod#x0002A; P = 0.122, #cod#x0003E; 0.05. Aging and Disease,null,8(3),301-313. Doi:10.14336/AD.2016.1018