Use of optics & Use of genetics

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Presentation transcript:

Use of optics & Use of genetics Optogenetics Use of optics & Use of genetics http://www.livescience.com/29340-optogenetics-brain-research-breakthrough-nsf-bts.html

Transgenic approaches are easy with fruit flies www.domyownpestcontrol.com

How could we target ChR to motor neurons? OK371-Gal4 x UAS-ChR2 In other words, what gene is OK371? Drosophila vesicular glutamate transporter gene (DVGLUT) that is expressed almost exclusively in neurons that release glutamate (Mahr and Aberle 2006).

Human Brain https://en.wikipedia.org/wiki/Serotonin

Adult Drosophila brain with neurons stained green and the neurotransmitter serotonin stained red.

Green are serotonin containing neurons http://www.mdpi.com/1422-0067/10/2/407/htm

Experiments Larva Motor neurons Examine acute behavioral changes

Mechanism of Channelrhodopsin2 in motor neurons Channelrhodopsin2 inactivated (normal light) Ca2+ ChR2 NO Muscle contraction

Mechanism of Channelrhodopsin2 in motor neurons Channelrhodopsin2 activated (blue light) Blue light Na+ Ca2+ ChR2 Glutamate Glutamate instead of Acetylcholine in Drosophila Muscle contraction

Blue light source setup

Bodywall contractions in larva

Group-1

Neuromuscular Junction ES Presynaptic terminal Go back and forth with blue light within PPT Synaptic cleft Postsynaptic target Glutamate Receptors

What would happen with the Minis at fly NMJ with ChR2 activation?

Lets do it !