From: Fate Maps of Neural Crest and Mesoderm in the Mammalian Eye Invest. Ophthalmol. Vis. Sci.. 2005;46(11):4200-4208. doi:10.1167/iovs.05-0691 Figure Legend: Detection of β-gal by immunohistochemistry in developing and mature extraocular muscles. β-gal expression (green) in presumptive extraocular muscles (PM) of E12.5 neural crest (A) and mesoderm (B) marked embryos. β-gal expression labels connective fascia cells (arrowheads) in developing (C) and mature (E) extraocular muscles of neural-crest–marked mice. β-gal expression labels myotubules and myofibers (arrows) in developing (D) and mature (F) extraocular muscles of mesoderm marked mice. Adult micrographs (E, F) are presented as the merge of red and green channels to enhance visualization of the myofibers. Date of download: 12/29/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.