CXCR6 and CCR5 Localize T Lymphocyte Subsets in Nasopharyngeal Carcinoma Greg Parsonage, Lee Richard Machado, Jan Wai-Ying Hui, Andrew McLarnon, Tilo.

Slides:

Advertisements

Similar presentations

Volume 94, Issue 2, Pages (March 2014)

Advertisements

Continuous Delivery of Neutralizing Antibodies Elevate CCL2 Levels in Mice Bearing MCF10CA1d Breast Tumor Xenografts Min Yao, Curtis Smart, Qingting.

Figure 1. CD11b+CD33+CD14+HLA-DR−/lo myeloid-derived suppressor cell expansion by human immunodeficiency virus.

Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor Induce Expression of CXCR4 on Human Endothelial Cells Rosalba Salcedo, Ken Wasserman,

Copyright © 2005 American Medical Association. All rights reserved.

Volume 129, Issue 4, Pages (October 2005)

Interleukin 12 Induces T-Cell Recruitment Into the Atherosclerotic Plaque by Xiaoyu Zhang, Alexander Niessner, Takako Nakajima, Wei Ma-Krupa, Stephen L.

Chemokine Receptor Expression on Neoplastic and Reactive T Cells in the Skin at Different Stages of Mycosis Fungoides Tilmann Kallinich, J. Marcus Muche,

Blockade of Glucocorticoid-Induced Tumor Necrosis Factor–Receptor-Related Protein Signaling Ameliorates Murine Collagen-Induced Arthritis by Modulating Follicular.

Volume 61, Issue 2, Pages (February 2012)

Engineering Human Peripheral Blood Stem Cell Grafts that Are Depleted of Naïve T Cells and Retain Functional Pathogen-Specific Memory T Cells Marie Bleakley,

Role of γ/δ T cells in a patient with CD4+ CD3– lymphocytosis, hypereosinophilia, and high levels of IgE Ilan Bank, MDa,d, Avner Reshef, MDb, Miriam.

Depletion of Alloreactive Donor T Lymphocytes by CD95-Mediated Activation-Induced Cell Death Retains Antileukemic, Antiviral, and Immunoregulatory T Cell.

Volume 49, Issue 1, Pages (January 2006)

Monocyte chemoattractant chemokines in cystic fibrosis

Blood-borne human plasma cells in steady state are derived from mucosal immune responses by Henrik E. Mei, Taketoshi Yoshida, Wondossen Sime, Falk Hiepe,

Absence of γ-Chain in Keratinocytes Alters Chemokine Secretion, Resulting in Reduced Immune Cell Recruitment Karolin Nowak, Daniela Linzner, Adrian J.

Impaired Responses of Peripheral Blood Mononuclear Cells to Staphylococcal Superantigen in Patients with Severe Atopic Dermatitis: A Role of T Cell Apoptosis

Volume 136, Issue 2, Pages (February 2009)

by Marlène Brandes, Katharina Willimann, Alois B

Prostaglandin E2 is a key factor for CCR7 surface expression and migration of monocyte-derived dendritic cells by Elke Scandella, Ying Men, Silke Gillessen,

Volume 132, Issue 1, Pages (January 2007)

Role of IL-9 in the pathophysiology of allergic diseases

Role of CCL17 in the Generation of Cutaneous Inflammatory Reactions in Hu-PBMC- SCID Mice Grafted with Human Skin Jules Gilet, Ying Chang, Cécile Chenivesse,

Increase in Activated Treg in TIL in Lung Cancer and In Vitro Depletion of Treg by ADCC Using an Antihuman CCR4 mAb (KM2760) Koji Kurose, MD, Yoshihiro.

Volume 132, Issue 7, Pages (June 2007)

Naive T cells sense the cysteine protease allergen papain through protease-activated receptor 2 and propel TH2 immunity Genqing Liang, PhD, Tolga Barker,

Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses by Cagan Gurer, Till.

Alternative Activation of Human Plasmacytoid DCs In Vitro and in Melanoma Lesions: Involvement of LAG-3 Chiara Camisaschi, Annamaria De Filippo, Valeria.

FLT3 ligand administration after hematopoietic cell transplantation increases circulating dendritic cell precursors that can be activated by CpG oligodeoxynucleotides.

Juan Liu, Erin Harberts, Antonella Tammaro, Nicholas Girardi, Renata B

IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis Rahul Purwar, Thomas Werfel, Miriam.

Myeloid-Derived Suppressor Cells in Psoriasis Are an Expanded Population Exhibiting Diverse T-Cell–Suppressor Mechanisms Lauren Y. Cao, Jin-Sung Chung,

Volume 132, Issue 3, Pages (March 2007)

Volume 141, Issue 3, Pages (September 2011)

Volume 138, Issue 3, Pages (March 2010)

Secretion of monocyte chemotactic protein-1 by human uterine epithelium directs monocyte migration in culture Richard A. Meter, M.D., Charles R. Wira,

Age-Related Differences in Sensitivity of Peripheral Blood Monocytes to Lipopolysaccharide and Staphylococcus Aureus Toxin B in Atopic Dermatitis Marie.

Effect of First-Line Treatment on Myeloid-Derived Suppressor Cells’ Subpopulations in the Peripheral Blood of Patients with Non–Small Cell Lung Cancer

Toll-Like Receptor-Mediated Upregulation of CXCL16 in Psoriasis Orchestrates Neutrophil Activation Sabine Steffen, Susanne Abraham, Maik Herbig, Franziska.

Suppression of natural killer cells in the presence of CD34+ blood progenitor cells and peripheral blood lymphocytes J. Clausen, M. Enk, B. Vergeiner,

Th17 Cytokines Stimulate CCL20 Expression in Keratinocytes In Vitro and In Vivo: Implications for Psoriasis Pathogenesis Erin G. Harper, Changsheng Guo,

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

Increase in Activated Treg in TIL in Lung Cancer and In Vitro Depletion of Treg by ADCC Using an Antihuman CCR4 mAb (KM2760) Koji Kurose, MD, Yoshihiro.

Volume 20, Issue 3, Pages (July 2017)

Dorothea Dijkstra, Holger Stark, Paul L. Chazot, Fiona C

Pivotal Role of Lesional and Perilesional T/B Lymphocytes in Pemphigus Pathogenesis Huijie Yuan, Shengru Zhou, Zhicui Liu, Weiting Cong, Xiaochun Fei,

Stimulation of Purinergic Receptors Modulates Chemokine Expression in Human Keratinocytes Saveria Pastore, Francesca Mascia, Sara Gulinelli, Sylvia Forchap,

Increased, but Functionally Impaired, CD14+ HLA-DR–/low Myeloid-Derived Suppressor Cells in Psoriasis: A Mechanism of Dysregulated T Cells David C. Soler,

Human Plasmacytoid Dendritic Cells Express Receptors for Anaphylatoxins C3a and C5a and Are Chemoattracted to C3a and C5a Ralf Gutzmer, Brigitta Köther,

Volume 135, Issue 1, Pages (July 2008)

Low Herpesvirus Entry Mediator (HVEM) Expression on Dermal Fibroblasts Contributes to a Th2-Dominant Microenvironment in Advanced Cutaneous T-Cell Lymphoma

Volume 29, Issue 1, Pages (July 2008)

Positive Selection of Dendritic Epidermal γδ T Cell Precursors in the Fetal Thymus Determines Expression of Skin-Homing Receptors Na Xiong, Chuhlo Kang,

Identification of a Novel CD160+CD4+ T-Lymphocyte Subset in the Skin: A Possible Role for CD160 in Skin Inflammation Sofia Abecassis, Jérôme Giustiniani,

Volume 17, Issue 2, Pages (February 2009)

Autocrine Regulation of Re-Epithelialization After Wounding by Chemokine Receptors CCR1, CCR10, CXCR1, CXCR2, and CXCR3 Kim L. Kroeze, Mireille A. Boink,

Kim L. Kroeze, Wouter J. Jurgens, Behrouz Z. Doulabi, Florine J

Human Beta-Defensin 3 Induces Maturation of Human Langerhans Cell–Like Dendritic Cells: An Antimicrobial Peptide that Functions as an Endogenous Adjuvant

Volume 24, Issue 6, Pages (June 2016)

Tumor Necrosis Factor-α- and IL-4-Independent Development of Langerhans Cell-Like Dendritic Cells from M-CSF-Conditioned Precursors Jean-Baptiste Barbaroux,

Volume 56, Issue 1, Pages (July 1999)

Volume 6, Issue 4, Pages (April 2016)

Lack of Membrane Expression of Interleukin-2 Receptor α Chain (CD25) in Mycosis Fungoides: Application of Laser Scanning Cytometry for Phenotyping of.

Moutih Rafei, Elena Birman, Kathy Forner, Jacques Galipeau

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Volume 132, Issue 7, Pages (June 2007)

CD11b-mediated migratory property of peripheral blood B cells

Interleukin-17 is Produced by Both Th1 and Th2 Lymphocytes, and Modulates Interferon-γ- and Interleukin-4-Induced Activation of Human Keratinocytes Cristina.

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Presentation transcript:

CXCR6 and CCR5 Localize T Lymphocyte Subsets in Nasopharyngeal Carcinoma Greg Parsonage, Lee Richard Machado, Jan Wai-Ying Hui, Andrew McLarnon, Tilo Schmaler, Meenarani Balasothy, Ka-Fai To, Alexander C. Vlantis, Charles A. van Hasselt, Kwok-Wai Lo, Wai-Lap Wong, Edwin Pun Hui, Anthony Tak Cheung Chan, Steven P. Lee The American Journal of Pathology Volume 180, Issue 3, Pages 1215-1222 (March 2012) DOI: 10.1016/j.ajpath.2011.11.032 Copyright © 2012 American Society for Investigative Pathology Terms and Conditions

Figure 1 CXCR6 and CCR5 expression on peripheral blood and tissue infiltrating lymphocytes. T cells were isolated from the blood (peripheral blood lymphocytes [PBL]) and tumor tissue (tumor infiltrating lymphocytes [TIL]) of nasopharyngeal carcinoma cases and multicolor flow cytometry used to determine the proportions of memory CD8+ (CD3+CD8+CD45RA−), CD4+ nonregulatory T cells (non-Tregs) (CD3+CD4+CD45RA−Foxp3−) and Tregs (CD3+CD4+CD45RA−CD25hiFoxp3+) expressing chemokine receptors (A) CXCR6 or (B) CCR5. Data from each patient were corrected for nonspecific staining measured using isotype-matched antibody controls. Statistical significance of differences was determined using the Mann-Whitney test (P values shown). C–D: Matched pair analysis for which the Wilcoxon matched pairs test was used. E–F: Representative plots of CXCR6 and CCR5 staining respectively on TIL (shaded) and matched PBL (thick line, not shaded) with concentration-matched isotype control staining shown with a thin line. The American Journal of Pathology 2012 180, 1215-1222DOI: (10.1016/j.ajpath.2011.11.032) Copyright © 2012 American Society for Investigative Pathology Terms and Conditions

Figure 2 Chemokine and chemokine receptor expression in nasopharyngeal carcinoma (NPC). NPC tissues were examined by immunohistochemistry for expression of the indicated chemokine receptors or chemokines. A–F: Representative staining from NPC cases is shown for CXCR6 (n = 9), CCR5 (n = 9), CXCL16 (n = 27), CCL5 (n = 17), and CCL4 (n = 33). Concentration-matched isotype control stains are shown as insets. The American Journal of Pathology 2012 180, 1215-1222DOI: (10.1016/j.ajpath.2011.11.032) Copyright © 2012 American Society for Investigative Pathology Terms and Conditions

Figure 3 Functional test of CXCR6 and CCR5 on freshly isolated tumor infiltrating lymphocytes. Infiltrating lymphocytes from four patients' diagnostic nasopharyngeal carcinoma biopsies (designated A, B, C, and D) were placed in porous transwell inserts (3-μm pore size) and presented with a gradient of the indicated chemokine CXCL16 (CXCR6 ligand) or CCL4 (CCR5 ligand) (10 ng/mL) or control medium. Migration index was calculated by dividing the numbers of T cells migrating in response to chemokine by the number migrating in response to medium alone. The American Journal of Pathology 2012 180, 1215-1222DOI: (10.1016/j.ajpath.2011.11.032) Copyright © 2012 American Society for Investigative Pathology Terms and Conditions

Figure 4 Functional chemokine secretion by a nasopharyngeal carcinoma (NPC) tumor cell line. A: Concentrations of CCL5 (open circles) and CXCL16 (filled squares) secreted by the NPC cell line c666.1 over a 72-hour period were measured by enzyme-linked immunosorbent assay. B: Dose-response curve showing chemotaxis of a CCR5 positive cell line (THP.1) in response to recombinant CCL5 or (C) c666.1-conditioned supernatants. D: shows THP.1 chemotaxis in response to 100 ng/mL recombinant CCL5 (filled bars) or neat conditioned medium from c666.1 cells (open bars), measured either in the presence of a negative control goat IgG or blocking antibody to CCL5 (5 μg/mL) as indicated. Data represent the mean of duplicate tests ± SD. The American Journal of Pathology 2012 180, 1215-1222DOI: (10.1016/j.ajpath.2011.11.032) Copyright © 2012 American Society for Investigative Pathology Terms and Conditions

Figure 5 CCR5 and CXCR6 expression on in vitro expanded EBV-specific T cell lines. EBV-specific T cell lines from healthy virus carriers were generated in vitro following stimulation with the autologous lymphoblastoid cell line. The expression of CCR5 and CXCR6 on EBV-specific CD8+ T cells (detected using human leukocyte antigen (HLA):peptide tetramers) was assessed by flow cytometry at various time points during the in vitro culture period. The HLA:peptide tetramers stained T cells specific for the following EBV antigens: CLG, HLA A*0201-restricted epitope in latent membrane protein-2 (sequence CLGGLLTMV); “HPV”, HLA B*3501-restricted epitope in Epstein-Barr virus nuclear antigen-1 (sequence HPVGEADYFEY); “QAK”, HLA B*0801-restricted epitope in EBNA-3A (sequence QAKWRLQTL); “IVT”, HLA A*1101-restricted epitope in EBNA-3B (sequence IVTDFSVIK). The American Journal of Pathology 2012 180, 1215-1222DOI: (10.1016/j.ajpath.2011.11.032) Copyright © 2012 American Society for Investigative Pathology Terms and Conditions