FIGURE 1 Scenarios for degradation of EG. (A) A simplified schema of EG. HA (orange); heparan sulfate (red; dermatan and chondroitin sulfates are not shown.

Slides:

Advertisements

Similar presentations

Fig. 1. Flow diagram of study participants and outcomes.

Advertisements

Fig. 1 ( A ) COX-2 mRNA abundance in hVSMC incubated without (−) and with (+) fetal calf serum (FCS, 2 h) and vehicle (control), cyclosporine A (CsA 100.

Fig. 3. Quantitative analysis of calcification at the aortic root showing the effect of long-term PD administration of PPi in mice . ( A ) Calcification.

Fig. 1. Serum activity of the osteoclast-specific enzyme TRAP-5b and serum levels of RANKL and OPG, key regulators involved in osteoclast differentiation.

FIGURE 1 Bivariate relationship between serum urea levels and serum Hcit levels, according to different nutritional regimens, namely FD, MD and VLPD. From:

Figure 1. Herbacetin binds to AKT1/2 and suppresses each respective kinase activity. The effect of herbacetin on (A) PI3K/AKT and (B) MAPK signaling pathway.

Fig. 4 Glucose concentration in the first 3 h of the dialysis session (blue circles) or equivalent time of the following day without dialysis (red circles).

FIGURE 1: The flow chart of the systematic review: paper retrieval and selection. From: Pregnancy in dialysis patients in the new millennium: a systematic.

FIGURE 1: Plasma levels of indoxyl sulfate (IS) in four groups: Sham, Sham + AST-120, CKD and CKD + AST-120 mice (n = 12 for each group). Data are expressed.

FIGURE 1 Flow chart of patient inclusion.

FIGURE 1 Trial profile. The safety population was defined as all randomly assigned patients who received at least one dose of study drug. All patients.

FIGURE 1: Urinary proteome analysis in nephrology

Molecular Therapy - Methods & Clinical Development

Fig. 1. Intrarenal resistance index (RI) by stage of CKD (classification followed the K/DOQI guidelines [ 2 ]). Each box shows the median, quartiles and.

Figure 2. Macrophages in dystrophic muscle in vivo and in vitro express Klotho. (A) A cross-section of 4-week-old mdx muscle labeled with antibodies to.

Figure 1. Herbacetin binds to AKT1/2 and suppresses each respective kinase activity. The effect of herbacetin on (A) PI3K/AKT and (B) MAPK signaling pathway.

Transplant: immunology and treatment of rejection

by Benjamin J. Frisch, John M. Ashton, Lianping Xing, Michael W

Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model

Cyclooxygenase Regulates Angiogenesis Induced by Colon Cancer Cells

Figure 1 The structure of the glycocalyx

Nat. Rev. Nephrol. doi: /nrneph

C. J. Ilse Raats, Jacob Van Den Born, Jo.H.M. Berden, M.D., Ph.D.

Volume 25, Issue 4, Pages (April 2014)

Exosomes/microvesicles as a mechanism of cell-to-cell communication

Figure 2 Mechanism of glycocalyx degradation

Volume 66, Issue 1, Pages (July 2004)

Transglutaminase 3 Protects against Photodamage

Volume 62, Issue 4, Pages (October 2002)

Effect of alternative peritoneal dialysis solutions on cell viability, apoptosis/necrosis and cytokine expression in human monocytes1 Joerg Plum, Mohammad.

International Journal of Cardiology

Volume 73, Issue 1, Pages (January 2008)

Figure 4 Podocyte–endothelial cross talk and activation of heparanase

Positive Selection from Newly Formed to Marginal Zone B Cells Depends on the Rate of Clonal Production, CD19, and btk Flavius Martin, John F. Kearney

Plant Stomata Function in Innate Immunity against Bacterial Invasion

Anne Marie W. Bartosch, Rick Mathews, John M. Tarbell

Figure 1 Experimental workflow and predictive modelling

Figure 1 Flow diagram detailing the systematic review process.

(A) Calcinosis cutis on the extensor side of the left upper arm ...

Localization of the Laminin α4 Chain in the Skin and Identification of a Heparin- Dependent Cell Adhesion Site Within the Laminin α4 Chain C-Terminal LG4.

Rapid Actin-Based Plasticity in Dendritic Spines

Figure 1 Tau promotes neuronal hypoactivity, even in the absence of tangles. (A) Schematic of the experimental ... Figure 1 Tau promotes neuronal hypoactivity,

Figure 1 KCTD16 antibodies are associated with an underlying tumour

Spatially and Temporally Regulated NRF2 Gene Therapy Using Mcp-1 Promoter in Retinal Ganglion Cell Injury Kosuke Fujita, Koji M. Nishiguchi, Yukihiro.

Figure 1. Expression of olfactory receptors in circumvallate taste papillae of mOR-M71-GFP (top row) and mOR-EG-GFP ... Figure 1. Expression of olfactory.

Marginal Zone and B1 B Cells Unite in the Early Response against T-Independent Blood-Borne Particulate Antigens Flavius Martin, Alyce M Oliver, John.

The Arabidopsis Transcription Factor AtTCP15 Regulates Endoreduplication by Modulating Expression of Key Cell-cycle Genes Li Zi-Yu , Li Bin , Dong Ai-Wu.

Volume 59, Issue 6, Pages (June 2001)

(A, B) ... (A, B) The frequencies of Tregs from patients with SLE (n = 50) and healthy controls (n = 20) are shown. (C) Data plots show the correlation.

Fateh Ouaaz, Joseph Arron, Ye Zheng, Yongwon Choi, Amer A Beg Immunity

Maya Capelson, Victor G. Corces Molecular Cell

(A, B) Representative TEM images from the skin ...

Figure 1 Immunohistochemistry for human cell differentiation molecules in samples of the right ventricular septum from ... Figure 1 Immunohistochemistry.

Rahul Raman, V. Sasisekharan, Ram Sasisekharan Chemistry & Biology

Bonnie E. Lonze, Antonella Riccio, Sonia Cohen, David D. Ginty Neuron

Volume 71, Issue 7, Pages (April 2007)

Volume 63, Issue 5, Pages (May 2003)

Volume 85, Issue 1, Pages (January 2014)

Volume 45, Issue 3, Pages (February 2012)

Volume 16, Issue 2, Pages (July 2016)

Figure 1. Ability of fluconazole to improve survival of G

Intestinal myofibroblasts in innate immune responses of the intestine

Teemu P. Miettinen, Mikael Björklund Cell Reports

Conformational Activation of Argonaute by Distinct yet Coordinated Actions of the Hsp70 and Hsp90 Chaperone Systems Kotaro Tsuboyama, Hisashi Tadakuma,

Figure 1. Sequential programmed death-ligand-1 (PD-(L)1) blockade and osimertinib schema of patients who developed ... Figure 1. Sequential programmed.

Vaccine MN confer protective innate and adaptive immunity.

Volume 9, Issue 3, Pages (November 2014)

Targeting Heparan Sulfate Proteoglycans as a Novel Therapeutic Strategy for Mucopolysaccharidoses Valeria De Pasquale, Patrizia Sarogni, Valeria Pistorio,

The cellular prion protein mediates neurotoxic signalling of β‐sheet‐rich conformers independent of prion replication Primary neurons lacking PrPC are.

Figure 1 The workflow of CAR development from a hybridoma

Presentation transcript:

FIGURE 1 Scenarios for degradation of EG. (A) A simplified schema of EG. HA (orange); heparan sulfate (red; dermatan and chondroitin sulfates are not shown for clarity). Note that diverse proteins are nested in EG, as those shown, as well as albumin and others (see text). GF, growth factors. (B) Heparanase-induced fragmentation of HS results in a partial degradation of EG. (C) Hyaluronidase-induced breakdown of HA chains results in a partial degradation of EG and an increase in permeability. (D) Sheddases cleave proteoglycans, syndecans and glypicans, resulting in peeling off of the entire EG structure. It should be noted that derangements in each component of EG lead in time to disorganization of other components. Hence, the schema depicts only the initial action of EG-degrading enzymes. Nonetheless, this latter scenario based on the action of sheddases results in a most severe and complete degradation of EG (akin to opening a can). From: Endothelial glycocalyx—the battleground for complications of sepsis and kidney injury Nephrol Dial Transplant. Published online May 23, 2017. doi:10.1093/ndt/gfx076 Nephrol Dial Transplant | © The Author 2017. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.

FIGURE 2 AFM nanoindentation. (A) A schematic diagram of the AFM assay FIGURE 2 AFM nanoindentation. (A) A schematic diagram of the AFM assay. Insert: a picture of the AFM head used for nanoindentation. The blue layer on the cell surface indicates the ESG. (B) Quantification of the thickness and stiffness of EG by AFM nanoindentation. From: Endothelial glycocalyx—the battleground for complications of sepsis and kidney injury Nephrol Dial Transplant. Published online May 23, 2017. doi:10.1093/ndt/gfx076 Nephrol Dial Transplant | © The Author 2017. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.

FIGURE 3 Representative STORM-acquired images of anti-HS-labeled EG and vWF distribution in controls and 10 min after LPS. The panels show three-dimensional reconstructed images of cultured endothelial cells stained with antibodies against HS and vWF. Note that preparations were nonpermeabilized so that vWF readily detectable after LPS is located extracellularly. Images display the height of EG and detectability of vWF in controls and 10 min after application of LPS (left panels) and side or top views. Note the readily detectable appearance of vWF 10 min after LPS, where it encroaches into the EG. The sites of detectable vWF also appear to be depleted of HS. From: Endothelial glycocalyx—the battleground for complications of sepsis and kidney injury Nephrol Dial Transplant. Published online May 23, 2017. doi:10.1093/ndt/gfx076 Nephrol Dial Transplant | © The Author 2017. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.

FIGURE 4 Schematic representation of a very early patchy loss of EG followed by the propagating innate immune response–driven global loss of EG. Exocytosed cargo molecules are represented in red. From: Endothelial glycocalyx—the battleground for complications of sepsis and kidney injury Nephrol Dial Transplant. Published online May 23, 2017. doi:10.1093/ndt/gfx076 Nephrol Dial Transplant | © The Author 2017. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.

FIGURE 5 (A) Whole-body EG volume and (B) survival of mice with the soil model of sepsis (FIP) with and without treatment with sulodexide. *P < 0.05. FIP, fecal-induced peritonitis; SDX, sulodexide; OGT, heparanase inhibitor OGT 2115. From: Endothelial glycocalyx—the battleground for complications of sepsis and kidney injury Nephrol Dial Transplant. Published online May 23, 2017. doi:10.1093/ndt/gfx076 Nephrol Dial Transplant | © The Author 2017. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.

FIGURE 6 A hypothetical view of the events linking and perpetuating the pathology of EG and endothelial dysfunction. From: Endothelial glycocalyx—the battleground for complications of sepsis and kidney injury Nephrol Dial Transplant. Published online May 23, 2017. doi:10.1093/ndt/gfx076 Nephrol Dial Transplant | © The Author 2017. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.