Invest. Ophthalmol. Vis. Sci ;49(4): doi: /iovs Figure Legend:

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From: Activated Inflammatory Infiltrate in HSV-1-Infected Corneas without Herpes Stromal Keratitis Invest. Ophthalmol. Vis. Sci.. 2008;49(4):1488-1495. doi:10.1167/iovs.07-1107 Figure Legend: Inflammatory cells infiltrate HSV-infected corneas without HSK but at reduced frequency compared to corneas with HSK. HSV-infected corneas with and without HSK at 15 dpi were disaggregated into single-cell suspensions and stained with anti-CD45, CD4, CD8α, CD11c, F4/80, and Gr-1 mAb. Cells were analyzed by flow cytometry. (A) Distinct live cell populations were identified based on FSC versus SSC, from which Gr-1bright (PMNs), CD11c+ DCs, F4/80+ macrophages, and CD4+ and CD8α+ T cells could be gated. Isotype controls were used to aid gating (data not shown). (B) The total number of infiltrating cells per cornea is shown. Data represent the average of at least eight corneas per group from two or more independent experiments. **P < 0.01; ***P < 0.001. Date of download: 12/30/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.