* * * Fig. S1 XB21-Os12g36180 Os11g43950 At4g12770 At4g

Slides:

Advertisements

Similar presentations

Supplemental Fig. S1 Ihsan et al., 2015 Supplemental Fig. S1 Sequence analysis of Solanaceous MAGO NASHI genes. ClustalW multiple alignments of nucleotide.

Advertisements

Figure S1. Genomic PCR of in vitro potato plants transformed with StPTB1 prom (top) and StPTB6 prom (bottom) constructs using nptII-specific primers. Thirty.

Enzyme activity (μmol mg protein-1 min -1 ) DAA Xu-142 Xu-142 fl mutant CIN VIN CWIN (C) Supplemental Figure 1 Control.

Figure S1. Effects of AVG, DIECA, DPI, NMMA, STA, and OKA on IbRPK expression in sweet potato (Ipomoea batatas cv. Tainung 57). Leaves with petiole cuts.

PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1 PfDGAT1-2 AtDGAT1 RcDGAT1 PfDGAT1-1.

Fig. S1. Amino acid sequence alignment of MYBS3 proteins. MYBS3 protein sequences of Arabidopsis thaliana (MYBH; NP_199550); (At3g16350; NP_188256), Glycine.

A) EF ATGGACAACTCAGCTCCAGACTCTTTACCTAGATCGGAAACCGCCGTCACCTACGACTCT 60 HM ATGGACAACTCAGCTCCGGACTCCTTACCTAGATCGGAAACCGCCGTCACCTACGACTCT 60.

P Xa13 :GUSP xa13 :GUS Supplemental Figure 1  -glucuronidase (GUS) expression regulated by the promoters of dominant Xa13 (P Xa13 ) and recessive xa13.

Louis et al. Figure S1 Mean expression intensity Figure S1. MPL1 expression in Arabidopsis organs The Genevestigator tool (

Figure S1. Co-expression network for MNM1: Shown is the co-expression network obtained from the ATTED-II database using MNM1 as a bait gene. The red dots.

Supplemental Fig. S1 A B AtMYBS aa AtMYBS

Supplemental Figure 1 A) B) C)

Volume 204, Issue 1, Pages (January 2011)

Fig. 2 Expression and subcellular localization of MSL8–GFP expressed from endogenous sequences. (A) Quantitative RT–PCR amplification of MSL8 transcripts.

Figure S5 Figure S5 Resistance assay of the OsNPR1-RNAi plants to rice bacterial blight pathogen Xoo. Gui99 is the untransformed control plant, and I1-I7.

Fig. S Fig. S2 Cre-mediated recombination in vivo. G2 mice displaying high levels of GFP were crossed.

Volume 6, Issue 4, Pages (October 2000)

by Wen-feng Xu, Zhi-wei Xie, Dominic W. Chung, and Earl W. Davie

Volume 16, Issue 9, Pages (May 2006)

Fractional Factorial Design

Volume 4, Issue 1, Pages (January 2011)

Volume 101, Issue 5, Pages (May 2000)

M. Ushita, T. Saito, T. Ikeda, F. Yano, A. Higashikawa, N. Ogata, U

Volume 5, Issue 1, Pages (January 2012)

Xiaofeng Cao, Steven E. Jacobsen Current Biology

Volume 13, Issue 1, Pages (July 2007)

Analysis of Telomerase Processivity

Volume 11, Issue 2, Pages (February 2018)

Volume 3, Issue 2, Pages (March 2010)

Volume 21, Issue 12, Pages (June 2011)

Alignment of H-NS, H-NS2, and StpA amino acid sequences.

Volume 6, Issue 5, Pages (September 2013)

Volume 64, Issue 4, Pages (October 2003)

The Salicylic Acid Receptor NPR3 Is a Negative Regulator of the Transcriptional Defense Response during Early Flower Development in Arabidopsis Zi Shi,

Volume 8, Issue 2, Pages (February 2015)

Volume 5, Issue 3, Pages (May 2012)

Volume 2, Issue 4, Pages (July 2009)

Volume 120, Issue 2, Pages (January 2005)

Amino acid alignment of TprK showing six different sequences in the T

Volume 4, Issue 3, Pages (May 2011)

FLS2 Molecular Cell Volume 5, Issue 6, Pages (June 2000)

Volume 13, Issue 16, Pages (August 2003)

Qiong A. Liu, Michael O. Hengartner Current Biology

Kristoffer Palma, Yuelin Zhang, Xin Li Current Biology

Designer TAL Effectors Induce Disease Susceptibility and Resistance to Xanthomonas oryzae pv. Oryzae in Rice Ting Li, Sheng Huang, Junhui Zhou, Bing.

insomniac and Cullin-3 Regulate Sleep and Wakefulness in Drosophila

AtABCG29 Is a Monolignol Transporter Involved in Lignin Biosynthesis

Volume 10, Issue 1, Pages (January 2017)

Posttranscriptional Gene Silencing Is Not Compromised in the Arabidopsis CARPEL FACTORY (DICER-LIKE1) Mutant, a Homolog of Dicer-1 from Drosophila E.Jean.

Mutants for UVH6 and MED14 are impaired in heat stress–induced release of silencing. Mutants for UVH6 and MED14 are impaired in heat stress–induced release.

Volume 6, Issue 5, Pages (September 2013)

Physcomitrella patens Auxin-Resistant Mutants Affect Conserved Elements of an Auxin- Signaling Pathway Michael J. Prigge, Meirav Lavy, Neil W. Ashton,

Arabidopsis WRKY45 Interacts with the DELLA Protein RGL1 to Positively Regulate Age-Triggered Leaf Senescence Ligang Chen, Shengyuan Xiang, Yanli Chen,

Volume 15, Issue 10, Pages (May 2005)

Design matrix Run A B C D E

Identification of the GCS1 ortholog in Gonium pectorale.

Volume 17, Issue 1, Pages (January 2007)

Volume 2, Issue 4, Pages (April 2002)

Volume 5, Issue 6, Pages (November 2012)

Volume 1, Issue 3, Pages (May 2008)

New microsome-associated HT-family proteins from Nicotiana respond to pollination and define an HT/NOD-24 protein family Kondo Katsuhiko , McClure Bruce.

Volume 121, Issue 4, Pages (May 2005)

Wang Long , Mai Yan-Xia , Zhang Yan-Chun , Luo Qian , Yang Hong-Quan

Volume 97, Issue 6, Pages (June 1999)

Characterization of Xanthomonas oryzae-Responsive cis-Acting Element in the Promoter of Rice Race-Specific Susceptibility Gene Xa13 Yuan Ting , Li Xianghua.

John Gray, Pam S Close, Steven P Briggs, Gurmukh S Johal Cell

Volume 7, Issue 12, Pages (December 2014)

Volume 1, Issue 5, Pages (September 2008)

Pst Activates Autophagy to Enhance Plant Susceptibility.

Volume 1, Issue 3, Pages (May 2008)

Presentation transcript:

* * * Fig. S1 XB21-Os12g36180 Os11g43950 At4g12770 At4g12780 87 97 94 96 * * * XB21-Os12g36180 Os11g43950 At4g12770 At4g12780 167 181 179 183 XB21-Os12g36180 Os11g43950 At4g12770 At4g12780 246 279 259 260 XB21-Os12g36180 Os11g43950 At4g12770 At4g12780 346 377 339 341 XB21-Os12g36180 Os11g43950 At4g12770 At4g12780 446 475 430 432 XB21-Os12g36180 Os11g43950 At4g12770 At4g12780 546 524 527 529 XB21-Os12g36180 Os11g43950 At4g12770 At4g12780 636 610 615 628 XB21-Os12g36180 Os11g43950 At4g12770 At4g12780 736 694 693 706 XB21-Os12g36180 Os11g43950 At4g12770 At4g12780 827 790 793 806 XB21-Os12g36180 Os11g43950 At4g12770 At4g12780 925 888 891 904

Supplementary Fig. 1 Amino acids sequence alignment of ALPs from rice (XB21-Os12g36180 and Os11g43950) and Arabidopsis (At4g12770 and At4g12780). Dashes indicate gaps. The numbers at each row to the right indicate the position in the sequence of the last amino acid in the row. Black-shaded residues are identical. ‘*’ and ‘box’ indicate an FxD/NxF motif and a DPF AP-2 binding motif, respectively. The line above the alignment notes the conserved sequence HPD that binds to Hsc70. The dashed line under the alignment indicates the auxilin-like-C-terminal domain including a J-domain.

Lesion length (cm) 12 DAI 10 DAI 25 20 15 10 5 XA21 Kit XA21 Kit Fig. S2 12 DAI 10 DAI 25 a ab 20 b ab abcd ab a ab abcde abc abcd 15 cde bcde def Lesion length (cm) 10 ef fg fg fg c 5 c fg g 1A 2A 3A 4A 5A 7A 8A 9A 10A 11A 12A 13A 14A 15A 16A 18A 19A 21A XA21 Kit XA21 Kit XB21ox/Kit XB21ox/Kit Supplementary Fig. 2 Overexpression of XB21 in Kitaake wild type plants exhibit enhanced resistance to Xoo. Lesion lengths measured from Kitaake plants overexpressing XB21 (XB21ox/Kit T0) (1A, 2A, 3A, 4A at 12 DAI and 5A, 7A, 8A, 9A, 10A, 11A, 12A, 13A, 14A, 15A, 16A, 18A, 19A, and 21A at 10 DAI), XA21 transgenic plants (23A-1-14), and Kitaake wild type plants. The error bars represent standard error of at least three leaves from each plant.

Fig. S3 Kit XA21 4A-42 5A-38 XB21ox/Kit Supplementary Fig. 3 Kitaake plants overexpressing XB21 (XB21ox/Kit) display cell death lesions. Kitaake wild type (Kit), transgenic rice carrying XA21 under the control of its native promoter (XA21, 23A-1-14), and Kitaake wild type overexpressing XB21 (XB21ox/Kit T1 lines, 4A-42 and 5A-38) were photographed six-weeks after planting.

Lesion length (cm) 25 20 15 10 5 XA21 Kit XB21ox/Kit Fig. S4 25 c bc c bc 20 bc b 15 Lesion length (cm) 10 a a a a 5 a a 31 33 34 36 39 42 32 40 41 43 32 33 34 35 37 38 39 41 31 36 42 31 31 32 33 34 35 37 32 36 37 38 31 33 31 XA21 Kit 4A 5A 7A 10A 10B 15A XB21ox/Kit Supplementary Fig. 4 T1 generation of XB21ox/Kit carrying XB21ox construct display enhanced resistance to Xoo. Lesion lengths measured 10 days after inoculation from Kitaake plants overexpressing XB21 (XB21ox/Kit T1) (progeny from 4A, 5A, 7A, 10A, 10B, and 15A), XA21 transgenic plants (23A-1-14), and Kitaake wild type plants. Progeny not carrying XB21ox construct are labeled with a white bar. The error bars represent standard error of at least three leaves from each plant.

XB21ox/Kit Kit 4A 5A 10B 31 32 33 37 31 32 XB21ox -+-++-+ XB21 Fig. S5 XB21ox/Kit 4A 5A 10B Kit 31 32 33 37 31 32 XB21ox -+-++-+ XB21 18S rRNA Supplementary Fig. 5 Transgenic Kitaake plants carrying XB21ox construct (XB21ox/Kit) overexpress XB21. Total RNA was extracted from four week old stage of XB21ox/Kit T1 lines (progeny from 4A, 5A, and 10B) and Kit wild type plants, and RT-PCR was performed with specific primers for XB21. Progeny carrying XB21ox construct was labelled with ‘+’ and non-transgenic segregants was labelled with ‘-’. Control RT-PCR reaction was carried out with 18S rRNA. Experimental results were repeated twice, with similar results.

Lesion length (cm) 18 16 14 12 10 8 6 4 2 Kit XA21 XB21ox/Kit Fig. S6 18 b 16 b b 14 12 10 Lesion length (cm) 8 a a 6 a 4 2 1 3 7 8 2 4 10 11 3 6 1 2 4 5 7 8 Kit XA21 5A-7 10A-1 XB21ox/Kit Supplementary Fig. 6 Overexpression of XB21 in Kitaake wild type plants (XB21ox/Kit, T2) displays resistant to a virulent strain of Xoo. Kitaake plants overexpressing XB21 (XB21ox/Kit T2 lines, progeny from 5A-4, 5A-7, 10A-1, and 10A-3), Kitaake wild type (Kit), and transgenic rice carrying XA21 under the control of its native promoter (XA21, 23A-1-14) were inoculated with Xoo. Lesion lengths of each plant were measured 12 days after inoculation. The error bars represent standard error of two to five leaves from each plant. Gray bar, progeny carrying XB21ox construct in XB21ox/Kit plants; White bar, progeny not carrying XB21ox construct

Lesion length (cm) 18 16 14 12 10 8 6 4 2 Kit XA21 XB21ox/Kit Fig. S6 18 b 16 b b 14 12 10 Lesion length (cm) 8 a a 6 a 4 2 1 3 7 8 2 4 10 11 3 6 1 2 4 5 7 8 Kit XA21 5A-7 10A-1 XB21ox/Kit Supplementary Fig. 6 Overexpression of XB21 in Kitaake wild type plants (XB21ox/Kit, T2) displays resistant to a virulent strain of Xoo. Kitaake plants overexpressing XB21 (XB21ox/Kit T2 lines, progeny from 5A-4, 5A-7, 10A-1, and 10A-3), Kitaake wild type (Kit), and transgenic rice carrying XA21 under the control of its native promoter (XA21, 23A-1-14) were inoculated with Xoo. Lesion lengths of each plant were measured 12 days after inoculation. The error bars represent standard error of two to five leaves from each plant. Gray bar, progeny carrying XB21ox construct in XB21ox/Kit plants; White bar, progeny not carrying XB21ox construct

Fig. S7 Kit XA21 2A-2 3A-11 5A-2 XB21ox/XA21 Supplementary Fig. 7 Overexpression of XB21 in XA21 plants (XB21ox/XA21) displays cell death lesions. Kitaake wild type (Kit), transgenic rice carrying XA21 under the control of its native promoter (XA21, 23A-1-14), and XA21 plants overexpressing XB21 (XB21ox/Kit T1 lines, 2A-2, 3A-11, and 5A-2) were photographed at six-week stage.

Lesion length (cm) 25 20 15 10 5 Kit XA21 XB21ox/XA21 Fig. S8 25 a 20 15 Lesion length (cm) 10 b b bc bc bc bc cd bcd bcd 5 cd d d d 2 4 7 11 14 1 5 8 9 10 12 13 3 6 1 11 2 4 7 9 12 13 14 3 2 4 14 3 5 6 7 9 11 12 1 8 10 13 3 13 1 6 9 12 14 11 Kit XA21 XB21ox/XA21 2A 3A 5A 6A Supplementary Fig. 8 T1 progeny from XB21 overexpressing XA21 transgenic plants (XB21ox/XA21) display cell death and enhanced resistance to Xoo. Lesion lengths measured 12 days after inoculation from XA21 plants overexpressing XB21 (XB21ox/Kit T1) (progeny from 2A, 3A, 5A, and 6A), XA21 transgenic plants (23A-1-14), and Kitaake wild type plants. The error bars represent standard error of at least three leaves from each plant. Gray bar, progeny carrying XB21ox construct in the absence of cell death; Black bar, progeny carrying XB21ox construct in the presence of cell death; White bar, progeny not carrying XB21ox construct.

XB21ox/XA21 Kit 2A 3A 5A 123 123 12 13 14 XB21ox -++-++-+-+ XB21 Fig. S9 XB21ox/XA21 2A 3A 5A Kit 123 123 12 13 14 XB21ox -++-++-+-+ XB21 18s rRNA Supplementary Fig. 9 XA21 plants carrying XB21ox construct (XB21ox/XA21) overexpress XB21. Total RNA was extracted from four-week old stage of XB21ox/Kit T1 lines (progeny from 4A, 5A, and 10B) and Kit wild type plants, and RT-PCR was performed with specific primers for XB21. Progeny carrying XB21ox construct was labelled with ‘+’ and non-transgenic segregants was labelled with ‘-’. Control RT-PCR reaction was carried out with 18S rRNA. Experimental results were repeated twice, with similar results.

Fig. S10 Forward Os11g43950 XB21-Os12g36180 595 682 Os11g43950 XB21-Os12g36180 663 756 Os11g43950 XB21-Os12g36180 731 830 Os11g43950 XB21-Os12g36180 805 904 Os11g43950 XB21-Os12g36180 879 978 Reverse Os11g43950 XB21-Os12g36180 952 1051 Supplementary Fig. 10 Comparison of partial nucleotide sequence used for XB21 RNA silencing and one of its closely related putative ALP Os11g43950. Arrow lines (labelled with Forward and Reverse) over XB21 sequence indicates XB21 specific primers used for PCR amplification and following construction.

Lesion length (cm) 12 DAI 10 DAI 25 20 15 10 5 XA21 Kit XA21 Kit Fig. S11 12 DAI 10 DAI 25 a 20 b a ab bc 15 bc ab ab ab bcd bcd ab Lesion length (cm) ab cd b 10 b de 5 e c 2A 2B 3A 3B 4B 5B 6A 7A 8A 9B 11A 12A 13A 14A 16A XA21 Kit XA21 Kit XB21RNAi/Kit XB21RNAi/Kit Supplementary Fig. 11 Silencing of XB21 in Kitaake plants does not exhibit significant difference in lesion lengths after Xoo inoculation Lesion lengths measured from Kitaake plants silencing XB21 (XB21RNAi/Kit T0) (2A/B, 3A/B, 4B, 5B, 6A and 7A at 12 DAI and 8A, 9B, 11A, 12A, 13A, 14A, and 16A at 10 DAI), XA21 transgenic plants (23A-1-14), and Kitaake wild type plants. The error bars represent standard error of at least three leaves from each plant.

Lesion length (cm) 30 25 20 15 10 5 XA21 Kit XB21RNAi/Kit Fig. S12 c 30 25 cd abd bcd abcd abcd abd 20 ab ab abd ab ae 15 Lesion length (cm) 10 e 5 7 9 10 11 1 2 4 6 3 1 2 3 5 6 4 3 10 11 1 2 1 2 3 1 2 3 4 5 6 2 4 6 XA21 Kit 3B 5B 7A 8A 12A 14A 16A XB21RNAi/Kit Supplementary Fig. 12 T1 generation of XB21RNAi/Kit do not display alteration of resistance to Xoo. Lesion lengths measured 12 days after inoculation from Kitaake plants overexpressing XB21 (XB21RNAi/Kit T1) (progeny from 3B, 5B, 7A, 8A, 12A, 14A, and 16A), XA21 transgenic plants (23A-1-14), and Kitaake wild type plants. Progeny not carrying XB21RNAi construct are labeled with a white bar. The error bars represent standard error of at least three leaves from each plant.