Micropropagation of Uraria picta (Jacq.) DC

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Presentation transcript:

Micropropagation of Uraria picta (Jacq.) DC Mafatlal M .Kher and M. Nataraj B.R.D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, Gujarat, 388120 Email: nattusamy@yahoo.com National Conference on Biodiversity and Its Role in Modern world

National Conference on Biodiversity and Its Role in Modern world Introduction Belongs to Fabaceae Common name Pithwan or Prishnaparni Distributed throughout India, China, Burma, Shrilanka (Yadav et al., 2009) Traditional uses Antidote to snake venom (Kirtikar and Basu, 1993) Cough and fever (Kirtikar and Basu,1993; Yusuf et al., 1994) Other property Antimicrobial ( Rahman et al2007) National Conference on Biodiversity and Its Role in Modern world

National Conference on Biodiversity and Its Role in Modern world Pharmaceutical products National Conference on Biodiversity and Its Role in Modern world

National Conference on Biodiversity and Its Role in Modern world As a result of this endangerment, in India the plant has been categorized as rare or vulnerable in the natural environment, making conservation of this plant crucial. Plant tissue culture in such cases has been shown to be useful method which allows mass multiplication under aseptic conditions and it is not dependent on season for availability of plant material. National Conference on Biodiversity and Its Role in Modern world

National Conference on Biodiversity and Its Role in Modern world In the present research work using this technique, the efforts were made to made to develop a method of rapid multiplication, by studying potential of coteledenory node as explant for micropropagation study. National Conference on Biodiversity and Its Role in Modern world

National Conference on Biodiversity and Its Role in Modern world Materials and Methods Media Preparation Plant Growth Regulators Surface sterilization Seed Germination Axillary shoot multiplication Subculture Rooting Culture condition National Conference on Biodiversity and Its Role in Modern world

National Conference on Biodiversity and Its Role in Modern world Seed germination Effect of Different concentrations of GA3 on seed germination of Uraria picta Six seeds per vessel with four replicates for each concentration National Conference on Biodiversity and Its Role in Modern world

Shoot multiplication and subculture National Conference on Biodiversity and Its Role in Modern world

Shoot multiplication and subculture Effect of Different concentrations of Kinetin and BAP on axillary shoot proliferation from coteledenory node of Uraria picta Four explant per vessel with six replicates for each concentration Effect of subculture on shoot multiplication each experiment with six replicate and four explant per culture bottle National Conference on Biodiversity and Its Role in Modern world

National Conference on Biodiversity and Its Role in Modern world Rooting Effect of different concentrations of NAA on in vitro rooting of Uraria picta each experiment with six replicate and four explant per culture bottle National Conference on Biodiversity and Its Role in Modern world

National Conference on Biodiversity and Its Role in Modern world Thank you all. National Conference on Biodiversity and Its Role in Modern world