5 millions cells/ml in 1.5 ml vials Plasma 6 vials

Slides:



Advertisements
Similar presentations
Endothelial Progenitor Cell (EPC) Core Laboratory Instructions Version 5.0 March 29, 2008 For questions regarding collection, processing, storage, or shipping.
Advertisements

Leukopak Processing.
Blood sample handling EHES Training Materials. Equipment Centrifuge Empty tubes for re- centrifugation (only needed if gel serum tubes are used) Storage.
IMMUNOLOGY LABORATORY PBMC ISOLATION SOP by Kizza D Martin Ssemambo
1 Vol. 01. p Vol. 01. p Vol. 01. p.20.
1 Vol. 03. p Vol. 03. p Vol. 03. p.21.
1 Vol. 02. p Vol. 02. p Vol. 02. p.19.
1 Vol. 03. p Vol. 03. p Vol. 03. p.16.
1 Vol. 02. p Vol. 02. p Vol. 02. p.30.
1 Vol. 03. p Vol. 03. p Vol. 03. p.35.
1 Vol. 02. p Vol. 02. p Vol. 02. p.10.
1 Vol. 01. p Vol. 01. p Vol. 01. p.14.
Orion QuikRead ® Portable CRP Test Kit. Supplies QuikRead ® 101 instrument Magnetic calibrator card Blood Capillary tubes with plungers (20 uL) Cuvettes.
SPIN
Buffy Coat Method Platelet Poor Plasma Step 1: Whole Blood is collected into a top and bottom pack and is centrifuged (hard spin) to produce platelet poor.
Lecture 6 Sample preparation.
Phase Difference = Phase Difference = 0.05.
Gas Exchange in Lungs Prof. K. Sivapalan. Properties of Gases 20132Gas Exchange.
CaTissue 2.0 Enhanced Specimen Event Tracking and Standard Operating Procedures Use Cases and UI Mockups.
:57 Bruker Barcode Label Types Introduction This SOP describes the software „Bruker Barcode Label Designer“ and the barcode labels for different.
Cryopreservation Proficiency Testing Program
Mesothelioma Collections September 2010 through September 2011.
The Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial Fifth Annual African-American Prostate Cancer Disparity Summit September 24, 2009 Christine.
Function of Blood Provides transport for the following: Nutrients Oxygen Carbon dioxide Waste products Hormones Heat Immune bodies.
Pictorial Guide To The RCV Procedure
Microhematocrit Determination. Microhematocrit Hematocrit — Test that provides a health care worker with an estimate of the patient’s red cell volume.
M M M M 5. Not Listed
What is DNA purification? Lyse cells or tissues Inactive endogenous nucleases Separate DNA from other cellular components – Proteins – Lipids – RNA – Others.

实验十四 紫外线灭菌  一、实验背景及目的:  灭菌是一种杀灭微生物的措施。紫外线灭菌 是物理灭菌因素之一。  1. 了解紫外线灭菌的原理;  2. 学习、掌握紫外线灭菌的方法。 版权所有 未经作者同意 请勿使用.
Sample Collection Bayer recommendations BNP is unstable in glass containers Recommended sample type is EDTA plasma using plastic tubes for sample collection.
Latent Heat of Phase Changes.  There are 4 phases (states) of matter.  Solid- definite shape and volume, has the least energy (ice)  Liquid- takes.
DNA extraction. Total DNA: whole blood (fresh or frozen), plasma, serum, buffy coat, body fluids, lymphocytes and cultured cells. This technology first.
BLOOD $ URINE COLLECTION
Biological Forensics.
Biospecimen Collection NRG Oncology Biospecimen Banks
It’s what the world is made of.
Blood The only fluid tissue in the human body
READING INCREMENTS.
Determination of Hematocrit value (H)
TUDA Progress.
States of Matter and Phase Changes
Microscopes and Other Tools
Laboratory Technique LAB.
Blood sample processing for serum total cholesterol, HDL cholesterol and plasma glucose, HbA1c and DNA extraction.
Phase Change.
The common lysis solutions contain A. sodium chloride.
BLOOD By: Sathish Rajamani Associate Professor. INTRODUCTION Blood is a connective tissue in fluid form. It is considered as the ‘Fluid of Life’, ‘fluid.
Specimen Processing and Analytical Nexus
Pictorial Guide To The RCV Procedure
Patient A.
محاضرة عامة التقنيات الحيوية (هندسة الجينات .. مبادئ وتطبيقات)
BLOOD and CIRCULATION.
Moisture in the Atmosphere
Phase Change – depends on heat energy
REDOXS Training: Introduction & Site Investigator
Plasma Clearance.
States of Matter and Phase Changes
OF STATES MATTER.
DNA Extraction from Blood
Phases Changes.
Chapter 13 States of Matter 13.2 The Nature of Liquids
Bs(hons)7th Bioinformatics UOG
Lecture 21: Vapor Pressure
Changes in State Chapter 8 - Lesson 2 pages
Sample Collection, Processing and Storage
Michelle Lane, Ph. D. , William B Schoolcraft, M. D
I have… I have… Who has 3:40? Who has 12:20? I have… I have…
Latent Heat of Phase Changes
COLLECT PATIENT BLOOD SAMPLE • Draw one (1) SST tube (Serum Separator Tube) from each patient using standard venipuncture technique • Allow blood to clot.
Presentation transcript:

5 millions cells/ml in 1.5 ml vials Plasma 6 vials ACD 2x7 ml (real volume) spin PBMC 3 vials 5 millions cells/ml in 1.5 ml vials Plasma 6 vials 1 ml each in 1.5 ml vials RBC and granulocytes EDTA 1x7 ml (real volume) 1x3ml (real vol) Buffy coat 1ml 3 vials,1 ml each in 1.5 ml cryovials 3vials, 1 ml each in 1.5 ml cryovials Serum 3 vials 1.5 ml, with 1 ml each 2 Blood cards DNA extraction 50 g 9 vials 1.5 ml with 1 g each and 2 vials with the rest (3 in Italy and 6 + 2 in USA) 7 ml Tube 3 vials 1.5 ml with 0,2 ml buffy each 6 vials 1.5 ml with 3 g RNA each vials Storage: : vapor phase of liquid nitrogen Shipping: vapor phase of liquid nitrogen Storage: -80°C Shipping: dry ice Storage: vapor phase of liquid nitrogen 125 l in one microtube 3 ml Tube 3 vials,whole blood,1 ml each in 1.5 ml cryovials 1 preanalitix tube (the other processed) Storage: -80° PreAnalytix Tube 2x2,5 ml (real volume) Storage: -20°C