Pseudomonas Identification Done By : Omniya Tayem Dr: Adbelraouf Elmanama Mohamad Laqan
Structure & Physiology Pseudomonas * Gram _ Negative rods Gram negative rod
Motile with polar flagella *
Motility positive
Obligate aerobe
Oxidase _ positive *
Do not ferment Carbohydrates
Resistant to multiple drugs
Majority of human infections _ P. aeruginosa
Common in the environment . Water . Air .Soil
Very simple nutritional requirements Many organic compound used as C & N sources , but only afew carbohydrates by oxidative metabolism
Some strains appear mucoid due to Polysaccharide Capsule
Some produce diffusible pigments Pyocyanin _ nonfluorescent bluish pigment Pyoverdin_ Fluorescent greenish pigment Pyorubin & Pyomelanin
Fluorescent Pigments White Light source Black Light source
Pseudomonas . aeruginosa
Pseudomonas aeruginosa colonies on Agar
Pseudomonas . aeruginosa
Identification of P. Aeruginosa Usually based on colonial morphology , B- hemolysis , Oxidase positivity , The presence of characteristic pigments & Sweet odor & Growth at 42c
Identification Of Pseudomonas Pigments * Pyocyanin (blue _ green ) Fluorescein (green _yellow_fluorescent ) Pyorubin (red _ brown )
Culures have fruity smell *
Biochemical reactions * .Gelatine hydrolysis .Starch hydrolysis .Carbohydrates use Trehalose (O_F medium) .Flourescent Pigment
Results
P. aeruginosa Gelatin hydrolysis +/ - ( variable result ) Starch hydrolysis + / - ( variable result ) Acid Aerobically from D_ Trehalose ( negative ) Fluorescent pigment ( positive ) Non Fluorescent pigment ( positive )
P .fluorescens Gelatin hydrolysis ( positive ) Starch hydrolysis ( negative ) Acid aerobically from D _ Trehalose (positive) Fluorescent pigment ( negative ) Non fluorescent pigment (positive )
P. Saccharophila Gelatin hydrolysis (P) Starch hydrolysis (P) Acid aerobically from D_ Trehalose (P ) Fluorescent pigment ( N ) Non fluorescent pigment ( N )
P. Putida Gelatine hydrolysis ( negative ) Starch hydrolysis (negative ) Acid aerobically from D_ Trehalose (N ) Fluorescent pigment ( P ) Non fluorescent pigment ( P )
The End