Pseudomonas Identification

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Presentation transcript:

Pseudomonas Identification Done By : Omniya Tayem Dr: Adbelraouf Elmanama Mohamad Laqan

Structure & Physiology Pseudomonas * Gram _ Negative rods Gram negative rod

Motile with polar flagella *

Motility positive

Obligate aerobe

Oxidase _ positive *

Do not ferment Carbohydrates

Resistant to multiple drugs

Majority of human infections _ P. aeruginosa

Common in the environment . Water . Air .Soil

Very simple nutritional requirements Many organic compound used as C & N sources , but only afew carbohydrates by oxidative metabolism

Some strains appear mucoid due to Polysaccharide Capsule

Some produce diffusible pigments Pyocyanin _ nonfluorescent bluish pigment Pyoverdin_ Fluorescent greenish pigment Pyorubin & Pyomelanin

Fluorescent Pigments White Light source Black Light source

Pseudomonas . aeruginosa

Pseudomonas aeruginosa colonies on Agar

Pseudomonas . aeruginosa

Identification of P. Aeruginosa Usually based on colonial morphology , B- hemolysis , Oxidase positivity , The presence of characteristic pigments & Sweet odor & Growth at 42c

Identification Of Pseudomonas Pigments * Pyocyanin (blue _ green ) Fluorescein (green _yellow_fluorescent ) Pyorubin (red _ brown )

Culures have fruity smell *

Biochemical reactions * .Gelatine hydrolysis .Starch hydrolysis .Carbohydrates use Trehalose (O_F medium) .Flourescent Pigment

Results

P. aeruginosa Gelatin hydrolysis +/ - ( variable result ) Starch hydrolysis + / - ( variable result ) Acid Aerobically from D_ Trehalose ( negative ) Fluorescent pigment ( positive ) Non Fluorescent pigment ( positive )

P .fluorescens Gelatin hydrolysis ( positive ) Starch hydrolysis ( negative ) Acid aerobically from D _ Trehalose (positive) Fluorescent pigment ( negative ) Non fluorescent pigment (positive )

P. Saccharophila Gelatin hydrolysis (P) Starch hydrolysis (P) Acid aerobically from D_ Trehalose (P ) Fluorescent pigment ( N ) Non fluorescent pigment ( N )

P. Putida Gelatine hydrolysis ( negative ) Starch hydrolysis (negative ) Acid aerobically from D_ Trehalose (N ) Fluorescent pigment ( P ) Non fluorescent pigment ( P )

The End