Figure 2. Sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of recombinant wild-type (WT) and mutant VSV L proteins. The WT.

Slides:

Advertisements

Similar presentations

Figure 1. Schematic of the corA leader mRNA

Advertisements

Table 1 Patient characteristics of attendees of the influenza vaccination, irrespective of AF status From: Yield of screening for atrial fibrillation in.

Figure 1. Unified models predicting gene regulation based on landscapes of gene-regulating factors. For each gene, position specific combinatorial patterns.

Fig. 8 The two cases for the terminal edge e<sub>0</sub>

Figure 1 Proportion of criteria chosen (as percentages, grouped into issues). From: Developing national obesity policy in middle-income countries: a case.

Figure 2. Number of cases required in a Mendelian randomization analysis with a binary outcome and a single instrumental variable for 80% power with a.

J Exp Bot. 2017;68(17): doi: /jxb/erx352

Figure 1. AsCpf1 and LbCpf1-mediated gene editing in human cells

Figure 3: MetaLIMS sample input.

Figure 2. Synthesis and execution of RNA CHA circuit

Figure 1 Study flow chart.

Figure 2. The graphic integration of CNAs with altered expression genes in lung AD and SCC. The red lines represent the amplification regions for CNA and.

Figure 1. A3B NTD mediates enzyme activity and oligomerization

From: Learning by Working in Big Cities

Fig. 1 Nodes in a conceptual knowledge graph

Figure 1: Pneumomediastinum and subcutaneous emphysema as indicated by the arrows. From: Pneumomediastinum and subcutaneous emphysema after successful.

From: qPrimerDepot: a primer database for quantitative real time PCR

Figure 2. Effects of 3′ and 5′ base mismatches on RNA templated DNA end-joining fidelity of PBCV-1 DNA ligase. For each PLP group, the contribution of.

Figure 1. drb7.2 mutant plants display altered accumulation of endoIR-siRNA. Wild-type (Col-0) and drb7.2 mutant plants were subjected to high throughput.

From: Detection of Bacteriuria by Canine Olfaction

Figure 1. Stabilization of the hairpin structure by non-nucleotide linkers (-L-) following enzymatic circularization. Structures of the stilbene diether.

Figure 2. Force and concentration dependence of the rate at which ScTopA initiates DNA relaxation. (A) The initial time lag (ITL) for ScTopA activity displays.

From: Introducing the PRIDE Archive RESTful web services

Figure 1 Overall analysis approach.

Figure 1. The flow chart illustrates the construction process of anti-CRISPRdb, and the information that users can obtain from anti-CRISPRdb. From: Anti-CRISPRdb:

Figure 1. MARF1 interacts with the DCP1:DCP2 mRNA decapping complex

Figure 2. Workflow of MethMotif Batch Query

From: Dynamic protein–RNA interactions in mediating splicing catalysis

Figure 1. RNA synthesis and capping are controlled by distinct residues in a loop structure of the VSV L PRNTase domain. (A) A 3D structural model of the.

Figure 3. Schematic representation of co-expression networks for BipA, SidD, and Erg11 encoding genes. Genes are represented by circles, with positive.

Figure 4. (A) Scatterplot of RPC4 T statistic (between TP0 and TP36) for the indicated groups of isolated tRNA genes (RPC4 peak only, n = 35; RPC4 + H3K4me3.

Figure 1. (A) The VEGF promoter PQS and scheme of G oxidation to OG, as well as (B) the proposed APE1-dependent pathway ... Figure 1. (A) The VEGF promoter.

Figure 1. An example of a thioviridamide-like molecule, thioalbamide, and inset, a proposed biochemical route to ... Figure 1. An example of a thioviridamide-like.

Figure 1. Overview of the workflow of NetworkAnalyst 3.0.

Figure 1. (A) Diagram of artificial microDNA creation by LAMA

Figure 1. Aminoacylation of 3′-NH2-tRNA catalyzed by flexizymes

FIGURE 1 The effect of daprodustat on hemoglobin (Hgb) levels

Figure 2. Effect of gradually decreasing photoperiod on PHA response in Siberian hamsters. Asterisk (*) indicates statistical significance at P﹤0.05, determined.

Figure 1. BRCA1-associated R-Loop accumulation at a non-coding region upstream of ESR1 locus. (A) Alignment of DRIP-seq ... Figure 1. BRCA1-associated.

Figure 1. Ratios of observed to expected numbers of exon boundaries aligning to boundaries of domain and disorder ... Figure 1. Ratios of observed to expected.

Figure 4. The mean of spermatocyte of various treatment groups

Fig. 1 Kaplan-Meier plot of cumulative incidence of cancer onset following dermatomyositis diagnosis stratified ... Anti-TIF1-Ab: anti-transcriptional.

Figure 1. Chemical structures of DNA and tc-DNA

Figure 1. DisA foci remain highly dynamic upon DNA damage in ΔrecO or ΔrecA cells. (A–F) Dynamic localization of ... Figure 1. DisA foci remain highly.

Point estimates with ... Point estimates with 95% CI. HR: hip replacement; KR: knee replacement. Unless provided in the caption above, the following copyright.

FIGURE 1 Participant flow diagram. Exercise Counseling Clinic (ECC).

Figure 6. The DNA lyase activity of hNTHL1 contributes to the processing of lesions in nucleosomes, even in the ... Figure 6. The DNA lyase activity of.

Figure 1. Analysis of human TRIM5α protein with Blast-Search and PhyML+SMS ‘One click’ workflow. (A) NGPhylogeny.fr ... Figure 1. Analysis of human TRIM5α.

Figure 1 Nelson-Aalen estimates of the cumulative incidence rates for patients on versus off IST. ON = optic neuritis; ... Figure 1 Nelson-Aalen estimates.

FIGURE 1 Study consort diagram

Figure 1. Illustration of DGR systems and their prediction using myDGR

Figure 1. The pipeline of Aggrescan3D 2.0 server.

Figure 1. Summary of experimental conditions and data normalization

Figure 4. Classified landsat image 2016

Figure 1. EBOV VP35 has NTP-binding and NTPase activities

Figure 1. Using Voronoi tessellation to define contacts

Figure 1. Designed cotranscriptional RNA structures

Figure 1. Analysis of the AP lyase activity of BsuLigD

Figure 4. RLS spectra of (A) TMPipEOPP and (B) OMHEPzEOPP in the presence of different concentrations of KRAS. The RLS ... Figure 4. RLS spectra of (A)

Figure 1. Uncertainty reduction, value creation, and appropriation in two case studies. Unless provided in the caption above, the following copyright applies.

Figure 1. (A) Architecture of Doc2Hpo. (B) Interactive user interface

Figure 1. MERMAID web server interface (Start page, Parameter page): MERMAID provides two ways to submit a protein ... Figure 1. MERMAID web server interface.

Figure 1. The framework of NetGO with seven steps

Figure 1. Overview of features that can be assessed in a single RegulationSpotter VCF analysis run. Depending upon a ... Figure 1. Overview of features.

Figure 1. Excess cost of methicillin-resistant Staphylococcus aureus (MRSA) compared with methicillin-susceptible S. ... Figure 1. Excess cost of methicillin-resistant.

Figure 1. 3C analysis of HEM3, BLM10, and SEN1 genes in rpb4Δ and isogenic wild type cells. (A) Schematic ... Figure 1. 3C analysis of HEM3, BLM10, and.

Figure 1. Prevalence of parasitic infection and anemia among the children. Unless provided in the caption above, the following copyright applies to the.

Figure 1. GWAS Catalog associations for coronary artery disease plotted across all chromosomes. Associations added ... Figure 1. GWAS Catalog associations.

Figure 1 Mechanisms of mitral regurgitation.

Figure 5. The endonucleolytic product from PfuPCNA/MR activity is displaced from dsDNA. Results from real-time ... Figure 5. The endonucleolytic product.

Presentation transcript:

Figure 2. Sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of recombinant wild-type (WT) and mutant VSV L proteins. The WT and mutant VSV L proteins (1 μg) were analyzed by 7.5% SDS-PAGE followed by Coomassie Brilliant Blue staining. M lanes show marker proteins with the indicated molecular masses. The names of the point mutants contain the original amino acid (one-letter code) at the indicated position in the VSV L protein followed by the replacement amino acid. The HR-RH mutant carrying the H1227R and R1228H mutations (lane 33) is a representative of the previously identified cap-defective mutants (16). From: Signature motifs of GDP polyribonucleotidyltransferase, a non-segmented negative strand RNA viral mRNA capping enzyme, domain in the L protein are required for covalent enzyme–pRNA intermediate formation Nucleic Acids Res. 2015;44(1):330-341. doi:10.1093/nar/gkv1286 Nucleic Acids Res | © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. 1