Invest. Ophthalmol. Vis. Sci ;48(7): doi: /iovs Figure Legend:

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From: Expression and Function of Toll-like Receptor-3 and -9 in Human Corneal Myofibroblasts Invest. Ophthalmol. Vis. Sci.. 2007;48(7):3069-3076. doi:10.1167/iovs.06-0968 Figure Legend: Chemokine production in corneal myofibroblasts detected by antibody array analysis. The appearance of representative arrays for factors in the culture supernatant of corneal myofibroblasts are shown in (A) and (B). GRO, IL-8, and MCP-1 proteins were detected in corneal myofibroblast culture medium. The corneal myofibroblasts also produced cytokines and growth factors such as IL-6, tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, and angiogenin. To examine whether signaling via TLR-9 or -3 enhanced chemokine-cytokine production, we compared myofibroblasts treated with CpG-DNA or poly (I:C) and cells without any treatment. The mean optical intensity of positive spots from the culture supernatants of cells incubated with CpG-DNA or poly (I:C) were compared with those from the culture supernatants of unstimulated cells. Stimulation of myofibroblasts with CpG-DNA enhanced the production of GRO, IL-6, and IL-8 (A). In contrast, stimulation with poly (I:C) enhanced the production of IL-6, IL-8, GRO, RANTES, ENA-78, and b-FGF (B). Date of download: 1/1/2018 The Association for Research in Vision and Ophthalmology Copyright © 2018. All rights reserved.