The draft genome, transcriptome, and microbiome of Dermatophagoides farinae reveal a broad spectrum of dust mite allergens Ting-Fung Chan, PhD, Kun-Mei.

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The draft genome, transcriptome, and microbiome of Dermatophagoides farinae reveal a broad spectrum of dust mite allergens Ting-Fung Chan, PhD, Kun-Mei Ji, PhD, Aldrin Kay-Yuen Yim, BS, Xiao-Yu Liu, MSc, Jun-Wei Zhou, PhD, Rui-Qi Li, BSM, Kevin Yi Yang, PhD, Jing Li, MS, Meng Li, MSc, Patrick Tik-Wan Law, PhD, Yu-Lan Wu, BS, Ze-Lang Cai, MSc, Hao Qin, PhD, Ying Bao, MSc, Ross Ka-Kit Leung, PhD, Patrick Kwok-Shing Ng, PhD, Ju Zou, MSc, Xiao-Jun Zhong, MSc, Pi-Xin Ran, MD, Nan-Shan Zhong, MSc, Zhi-Gang Liu, MD, Stephen Kwok-Wing Tsui, PhD Journal of Allergy and Clinical Immunology Volume 135, Issue 2, Pages 539-548 (February 2015) DOI: 10.1016/j.jaci.2014.09.031 Copyright © 2014 The Authors Terms and Conditions

Fig 1 HDM morphology and phylogeny. A, Photomicrograph of a live adult female D farinae mite. B, Scanning electron microscopic image of an adult female D farinae mite. C, GO distribution of D farinae. D, Phylogenetic tree of D farinae with 25 other arthropod species. Journal of Allergy and Clinical Immunology 2015 135, 539-548DOI: (10.1016/j.jaci.2014.09.031) Copyright © 2014 The Authors Terms and Conditions

Fig 1 HDM morphology and phylogeny. A, Photomicrograph of a live adult female D farinae mite. B, Scanning electron microscopic image of an adult female D farinae mite. C, GO distribution of D farinae. D, Phylogenetic tree of D farinae with 25 other arthropod species. Journal of Allergy and Clinical Immunology 2015 135, 539-548DOI: (10.1016/j.jaci.2014.09.031) Copyright © 2014 The Authors Terms and Conditions

Fig 2 Proteomics discovery of UQCRB-like protein and IgE-binding assays. A, Two-dimensional polyacrylamide gel electrophoresis (isoelectric point range, 3.0-10.0) demonstrating 13 IgE-immunopositive spots (red circles). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis results for each spot can be found in Table E10. B, Corresponding IgE-immunoblot image. C, Tandem mass spectrometry (MS/MS) spectrum of spot no. 3 matching peptide fragment YGLYYDDFYDYTDAAHLEAVR of the mite UQCRB-like protein; charts show intensity (relative abundance) of motifs as a function of m/z. D, Another MS/MS spectrum of spot no. 3 identical to peptide fragment LPPDLYDQHTYR of the mite UQCRB-like protein. E, Deduced amino acid sequence of the mite UQCRB-like protein (GenBank accession no.: KC669700). Boxes indicate the 2 fragments in MS/MS spectrums. F, Binding of recombinant UQCRB-like protein by IgE in sera from 18 of 18 patients with HDM allergy. MW, Molecular weight. G, ELISA demonstrating IgE binding to recombinant UQCRB-like protein (Z = 5.6702, P < .0001) in 22 (100%) of 22 sera from patients with HMD sensitization (red circles) and 22 nonallergic subjects (blue squares) as controls. Journal of Allergy and Clinical Immunology 2015 135, 539-548DOI: (10.1016/j.jaci.2014.09.031) Copyright © 2014 The Authors Terms and Conditions

Fig 2 Proteomics discovery of UQCRB-like protein and IgE-binding assays. A, Two-dimensional polyacrylamide gel electrophoresis (isoelectric point range, 3.0-10.0) demonstrating 13 IgE-immunopositive spots (red circles). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis results for each spot can be found in Table E10. B, Corresponding IgE-immunoblot image. C, Tandem mass spectrometry (MS/MS) spectrum of spot no. 3 matching peptide fragment YGLYYDDFYDYTDAAHLEAVR of the mite UQCRB-like protein; charts show intensity (relative abundance) of motifs as a function of m/z. D, Another MS/MS spectrum of spot no. 3 identical to peptide fragment LPPDLYDQHTYR of the mite UQCRB-like protein. E, Deduced amino acid sequence of the mite UQCRB-like protein (GenBank accession no.: KC669700). Boxes indicate the 2 fragments in MS/MS spectrums. F, Binding of recombinant UQCRB-like protein by IgE in sera from 18 of 18 patients with HDM allergy. MW, Molecular weight. G, ELISA demonstrating IgE binding to recombinant UQCRB-like protein (Z = 5.6702, P < .0001) in 22 (100%) of 22 sera from patients with HMD sensitization (red circles) and 22 nonallergic subjects (blue squares) as controls. Journal of Allergy and Clinical Immunology 2015 135, 539-548DOI: (10.1016/j.jaci.2014.09.031) Copyright © 2014 The Authors Terms and Conditions

Fig 3 Endosymbiotic microbes and bacteriolytic enzyme. A, Distribution of bacterial genera living in D farinae. Endophytic, Unclassified bacteria. B, Distinctive labeling of gut contents with anti–E cloacae antibody (left) and no-antibody controls (right). C, Structure of the D farinae gene (DEFA_122470) encoding 13.8-kDa bacteriolytic enzyme (2 exons and 1 intron). UTR, Untranslated region. Journal of Allergy and Clinical Immunology 2015 135, 539-548DOI: (10.1016/j.jaci.2014.09.031) Copyright © 2014 The Authors Terms and Conditions

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