Contribution of nitric oxide to the pathogenesis of cirrhotic cardiomyopathy in bile duct– ligated rats Hongqun Liu, Zenghua Ma, Samuel S. Lee Gastroenterology

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Contribution of nitric oxide to the pathogenesis of cirrhotic cardiomyopathy in bile duct– ligated rats Hongqun Liu, Zenghua Ma, Samuel S. Lee Gastroenterology Volume 118, Issue 5, Pages 937-944 (May 2000) DOI: 10.1016/S0016-5085(00)70180-6 Copyright © 2000 American Gastroenterological Association Terms and Conditions

Fig. 1 RT-PCR of NOS2 transcription from rat hearts. Lane 1, 1-kilobase (kb) ladder; lanes 2 and 3, control; lanes 4 and 5, BDL; lane 6, lipopolysaccharide (LPS)-treated positive control. Computerized optical densitometry indicated a significant difference between controls (n = 6) and BDL (n = 6) (P < 0.01). Gastroenterology 2000 118, 937-944DOI: (10.1016/S0016-5085(00)70180-6) Copyright © 2000 American Gastroenterological Association Terms and Conditions

Fig. 2 Western blot analysis of NOS2 protein expression in hearts. Lane 1, lipolysaccharide endotoxin treatment as a positive control; lanes 2 and 3, sham-operated controls; lanes 3 and 4, BDL. Computerized optical densitometry showed a significant difference between controls (n = 6) and BDL (n = 6) (P < 0.05). Gastroenterology 2000 118, 937-944DOI: (10.1016/S0016-5085(00)70180-6) Copyright © 2000 American Gastroenterological Association Terms and Conditions

Fig. 3 Imunohistochemical staining for NOS2. No immunoprecipitate is observable on the slide from the sham control. In the cirrhotic heart, NOS2-positive cells are localized in cardiomyocytes (original magnification 50×). Gastroenterology 2000 118, 937-944DOI: (10.1016/S0016-5085(00)70180-6) Copyright © 2000 American Gastroenterological Association Terms and Conditions

Fig. 4 RT-PCR of NOS3 transcription from rat hearts. Lane 1, 1-kb ladder; lanes 2 and 3, control; lanes 4 and 5, BDL. Computerized optical densitometry indicated no significant difference between controls (n = 6) and BDL (n = 6). Gastroenterology 2000 118, 937-944DOI: (10.1016/S0016-5085(00)70180-6) Copyright © 2000 American Gastroenterological Association Terms and Conditions

Fig. 5 Western blot analysis of NOS3 protein expression in hearts. Lane 1, NOS3 from human endothelium as a positive control; lanes 2 and 3, sham-operated controls; lanes 4 and 5, BDL. Computerized optical densitometry showed no significant difference between controls (n = 6) and BDL (n = 6). kDa, kilodaltons. Gastroenterology 2000 118, 937-944DOI: (10.1016/S0016-5085(00)70180-6) Copyright © 2000 American Gastroenterological Association Terms and Conditions

Fig. 6 Cumulative dose-responses to isoproterenol in isolated left ventricular papillary muscles from BDL and sham-operated rats (n = 6 for each of the 4 groups). Maximal response (Rmax) for the BDL group was significantly lower than the others. No significant differences were observed in EC50 values among the 4 groups. Gastroenterology 2000 118, 937-944DOI: (10.1016/S0016-5085(00)70180-6) Copyright © 2000 American Gastroenterological Association Terms and Conditions

Fig. 7 Effects of the exogenous NO donor SNAP on isoproterenol-stimulated maximal papillary muscle contractility. Contractility was significantly decreased in the SNAP-treated group (n = 6 in each group). *P < 0.05 compared with control. Gastroenterology 2000 118, 937-944DOI: (10.1016/S0016-5085(00)70180-6) Copyright © 2000 American Gastroenterological Association Terms and Conditions

Fig. 8 Effects of IL-1β and L-NAME on isoproterenol-stimulated maximal left ventricular papillary muscle contractility. Decreased contractile force with IL-1β was reversed by L-NAME coincubation (n = 6 in each group). *P < 0.05 compared with control and IL-1β+L-NAME group. Gastroenterology 2000 118, 937-944DOI: (10.1016/S0016-5085(00)70180-6) Copyright © 2000 American Gastroenterological Association Terms and Conditions