Important Extra Information Doctors slides Doctors notes

Slides:



Advertisements
Similar presentations
Cerebrospinal fluid Definition; It is the water clear fluid surrounding the brain and spinal cord. Formation; The cerebrospinal fluid is formed as an ultra.
Advertisements

C EREBROSPINAL FLUID IN CLINICAL BIOCHEMISTRY LAB Ramzi Shawahna, PhD An-Najah National University.
Cerebrospinal Fluid (CSF) Analysis for total protein
TESTS ON AMINO ACIDS AND PROTEINS
CEREBROSPINAL FLUID BY Hossam HASSAN
DETERMINATION OF BLOOD GLUCOSE CONCENTRATION
COLORIMETRYCOLORIMETRY Prepared By Michigan Department of Environmental Quality Operator Training and Certification Unit Note: A printed description of.
Methods to Detect Red Cell Membrane Disorders
Methods to Detect Red Cell Membrane Disorders
URINALYSIS Finding the Clues Hidden in Urine
Colorimetry & Spectrophotometry.
Cerebrospinal fluid CSF.
CSF ANALYSIS.
An introduction to Urinalysis as performed in the Clinical Laboratory.
Determination of Total Serum Proteins By Biuret Method
Practical Hematology Lab
Serum biochemical parameters glucose assay Biochemistry Clinical practice CLS 432 Dr. Samah Kotb Lecturer of Biochemistry 2015.
B IOCHEMISTRY P RACTICAL By: Dr. Beenish Zaki. CSF A NALYSIS.
CLS 332 CLINICAL INSTRUMENTAL ANALYSIS. A VISIBLE ABSORPTION SPECTROMETER.
It ’ s not just water ! An introduction to Urinalysis as performed in the Clinical Laboratory.
Spectrophotometry Ability of molecules to absorb and transmit light energy is the basis of one of the most widely used procedures for determining the concentration.
Safety in Chemical Laboratories. Introduction 1. A chemical lab is potentially hazardous environment 2. Accident and injury can happen anytime 3. Lab.
The students should be able to understand & become familiar with: 1) General safety rules followed in Biochemistry laboratory 2)Safety with laboratory.
ABOUT CSF Cerebrospinal fluid (CSF) was first examined in the 19th century using primitive techniques (eg, sharpened bird quills).
CYTOPATHOLOGY-14 DR. MAHA AL-SEDIK. CEREBROSPINAL FLUID (CSF) A. Composition and formation: 1. CSF is one of the major fluid of the body a. Adult total.
BODY FLUIDS: Cerebrospinal Fluid
 Visual exam A laboratory technician will examine the urine's appearance. Urine is typically clear. Cloudiness or unusual  odor may indicate a problem.
KAU-Faculty of Science- Biochemistry department Clinical biochemistry lab (Bioc 416) 2012 T.A Nouf Alshareef
COLORIMETRY & SPECTROPHOTOMETR
Identification of Normal Physical and Chemical Urine Constituents
Lab1 A VISIBLE ABSORPTION SPECTROMETER. -One of the simplest and most widely used methods to determine concentration of a substance in solution -Measures.
URINALYSIS LAB 1.
Determination Of Albumin In Serum By Bromocresol Green Method
Spectrophotometry Measuring Concentrations of Substances in Body Fluids.
Finding the Clues Hidden in Urine
Clinical laboratory instrumentation
Finding the Clues Hidden in Urine
Lab1 A VISIBLE ABSORPTION SPECTROMETER
Triglyceride determination
CSF ANALYSIS.
Human Anatomy of the Urinary System
BODY FLUIDS: Cerebrospinal Fluid (CSF)
Performing the Urinalysis
Practical Analysis Using Spectrophotometer
322 BCH Method of Enzyme Assay.
Important note biochem
Cerebrospinal Fluid (CSF) Analysis for total protein
Testing Urine with a Urine Reagent Strip PP6
Methods to Detect Red Cell Membrane Disorders
Determination of Protein Concentration of a Solution
Finding the Clues Hidden in Urine
Finding the Clues Hidden in Urine
Quantitative of protein
Quantitative Proteins Estimation by Lowry method
Practical Hematology Lab Osmotic Fragility Test
Terry Kotrla, MS, MT(ASCP) Professor Austin Community College
Purpose of the Lab 3E, 3F, 3H Learn how to use a spec to figure out concentration of solutions Learn how to use a GLX Practice application of calculations.
QUANTITATION OF METHEMOGLOBIN
Finding the Clues Hidden in Urine
Finding the Clues Hidden in Urine
BODY FLUIDS: Cerebrospinal Fluid
Finding the Clues Hidden in Urine
Quantitative Proteins Estimation by lowry method
BODY FLUIDS: Cerebrospinal Fluid
Practical Hematology Lab Osmotic Fragility Test
BODY FLUIDS: Cerebrospinal Fluid (CSF)
Practical Hematology Lab Osmotic Fragility Test
Finding the Clues Hidden in Urine
Bilirubin.
Clinical instrumental analysis
Presentation transcript:

Important Extra Information Doctors slides Doctors notes " ما تعثرت ارتباكا يا حصى .. او جنوحا لإختصار الالف ميل  ليس ذنبي أن دربي ما استوى..  كان ذنبي ثوب احلامي طويل! " جلال الدين الرومي Biochemistry Cerebrospinal Fluid (CSF) Analysis for total protein

CSF Examination Report: Physical examination: Color Appearance Viscosity Chemical examination: CSF protein concentration (g/L) Group number & Student names.

Indications for CSF analysis CSF examination Indications for CSF analysis CNS infections Demyelinating diseases malignancy Hemorrhage in CNS Autoimmune disorders The following examinations are performed for CNS samples: Physical examination Microscopic examination Chemical examination Microbiological test

1- Physical Examination: Parameter Normal abnormal Volume 100-150 ml (8% of CNS cavity volume) Turbidity Clear Cloudy/turbid- may indicate the presence of: white, or red blood cells, microorganisms, or an increase in protein level. Color Colorless Changes in color may point to additional substances in the fluid. Yellow (Xanthochromia)1, orange-brown or red may indicate the presence of blood or hemoglobin pigments2. Viscosity3 same consistency as water Thicker CSF may be seen in patients with certain types of cancers or meningitis. Xanthochromia is the yellow discoloration indicating the presence of bilirubin in the (CSF). The two most common reasons for blood and hemoglobin pigments in CSF are: traumatic tap (color is bright and red and erythrocytes number is decreased. subarachnoid hemorrhage (SAH) = xanthochromia is present. you should shake the sample to inspect its viscosity.

2- Chemical Analysis: Remember !! Centrifuge: Before any analysis, the fluid should be centrifuged to avoid contamination by cellular elements. CSF is the most precious biological material. Often, only small volumes of CSF are available for analysis due to difficulty in collection; hence handle this with care. The specimen may not be pre-tested for micro-organisms ( may contain virulent organisms), so strict safety precautions should be followed. Routinely performed biochemical tests in CSF are: Total protein (total- specific e.g. albumin & Immunoglobulin) glutamine and acid-base parameters lactate lactate dehydrogenase glucose protein is normally low in CSF: if increased suspect infection, but its decrease is not significant. lactate and lactate dehydrogenase increase in bacterial infection, but not in viral. glutamine increase in CSF due to liver disease

Abnormal findings of CSF in some pathological conditions Mind map Turbidity Physical Examination Color Viscosity CSF Protein Assay Spectrophotometer glucose Cerebrospinal Fluid (CSF) protein (total and specific) Chemical Analysis Procedure Mind map by 432 biochemistry team lactate Calculation lactate dehydrogenase Normal Range Abnormal findings of CSF in some pathological conditions glutamine and acid-base parameters

CSF Protein Assay Spectrophotometer “Extra Information” Protein present in the CSF is detected by a series of enzymatic reactions that ultimately form a colored product like kit based on Biuret method. Biuret reagent when interacts with the peptide bonds in the protein give a blue coloured product. The intensity of the color is proportional the amount of protein in CSF Color intensity is determined by measuring the absorbance by the colored solution at a wavelength of 546nm Absorbance is measured by an instrument, spectrophotometer Spectrophotometer “Extra Information” Most of visible spectrophotometers are composed of: Light source which works with visible wavelengths (400-700 nm) Monochromator filter for choosing desired wavelength Sample holder (cuvette) Detector Meter or recorder

Tuberculous Meningitis Abnormal findings of CSF in some pathological conditions Very important Condition Parameter Brain tumor Viral Meningitis Tuberculous Meningitis Bacterial Meningitis (pyogenic) ↑ Normal ↑↑ Proteins ↓ Normal or slightly ↓ ↓↓ Glucose Normal or ↓ Chloride

Normal reference values for CSF protein: Procedure Normal Range Normal reference values for CSF protein: 15 - 45 mg/dL ( 0.1 - 0.4 g/L ) Test Standard Blank Reagent 2 ml CSF sample 40 l - Dist.H2O Mix and incubate for 15 minutes at room temperature Measure absorbance at 546 nm Calculation Protein concentration (g/L) = Absorbance of sample ”test” X Conc. of standard (60 g/L) Absorbance of standard Important: you must convert from mg/dL to g/L ) , divide on 100 .

Case 1 Case scenarios by 433 team

Case 2

PLEASE CONTACT US IF YOU HAVE ANY ISSUE THANK YOU FOR CHECKING OUR WORK TEAM LEADERS Mohammad Almutlaq Atikah kadi