ANP GAPDH CTL CTL+MB DB DB+MB CTL CTL+MB DB DB+MB

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ANP GAPDH 15 38 CTL CTL+MB DB DB+MB CTL CTL+MB DB DB+MB Supplementary Figure 1. MB treatment reduces atrial natriuretic peptide (ANP) in the DB heart (upper panel). Densitometric analysis is shown as reported to GAPDH as the loading control (lower panel). * p<0.05. CTL = Control (N=3), CTL+MB = Control + Methylene Blue (N=3), DB = Diabetic (N=3), DB+MB = Diabetic + Methylene Blue (N=3). Mean ± SEM. kDa 15 38 ANP GAPDH CTL CTL+MB DB DB+MB p=0.06 ANP/GAPDH CTL CTL+MB DB DB+MB