Figure 1: Binding displacement curves of serially diluted pooled-faecal extracts against the cortisol standard to validate the enzyme immunoassay. The.

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Figure 1: Binding displacement curves of serially diluted pooled-faecal extracts against the cortisol standard to validate the enzyme immunoassay. The y-axis shows the percentage of hormone bound/total binding. The 50% binding point (represented by the dashed line) determined the dilution factor (1:16) for Melbourne Zoo tiger faecal extracts. From: Evaluating physiological stress in Sumatran tigers (Panthera tigris ssp. sumatrae) managed in Australian zoos Conserv Physiol. 2014;2(1). doi:10.1093/conphys/cou038 Conserv Physiol | © The Author 2014. Published by Oxford University Press and the Society for Experimental Biology.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse distribution and reproduction in any medium, provided the original work is properly cited.

Figure 2: Regression plot for recovery of cortisol standard in the extract pool to achieve extraction efficiency (extraction efficiency = 99%). From: Evaluating physiological stress in Sumatran tigers (Panthera tigris ssp. sumatrae) managed in Australian zoos Conserv Physiol. 2014;2(1). doi:10.1093/conphys/cou038 Conserv Physiol | © The Author 2014. Published by Oxford University Press and the Society for Experimental Biology.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse distribution and reproduction in any medium, provided the original work is properly cited.

Figure 3: Individual faecal cortisol metabolite profiles obtained from enzyme immunoassays on faecal samples collected from tigers (n = 5) at Melbourne Zoo (Victoria) over a 2 month period. One data point was excluded from statistical analysis because it was attributed to the unnatural stress event of darting and anaesthesia of Female 1. From: Evaluating physiological stress in Sumatran tigers (Panthera tigris ssp. sumatrae) managed in Australian zoos Conserv Physiol. 2014;2(1). doi:10.1093/conphys/cou038 Conserv Physiol | © The Author 2014. Published by Oxford University Press and the Society for Experimental Biology.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse distribution and reproduction in any medium, provided the original work is properly cited.

Figure 4: Mean faecal cortisol metabolite concentration (±1 SEM) for Melbourne Zoo's tigers (n = 5), clustered by sex. From: Evaluating physiological stress in Sumatran tigers (Panthera tigris ssp. sumatrae) managed in Australian zoos Conserv Physiol. 2014;2(1). doi:10.1093/conphys/cou038 Conserv Physiol | © The Author 2014. Published by Oxford University Press and the Society for Experimental Biology.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse distribution and reproduction in any medium, provided the original work is properly cited.

Figure 5: Mean faecal cortisol metabolite values (±1 SEM) for male (blue) and female tigers (green) at Melbourne Zoo (n = 2 males and 3 females), Dreamworld (n = 8 males and 5 females) and Australia Zoo (n = 4 males and 4 females). From: Evaluating physiological stress in Sumatran tigers (Panthera tigris ssp. sumatrae) managed in Australian zoos Conserv Physiol. 2014;2(1). doi:10.1093/conphys/cou038 Conserv Physiol | © The Author 2014. Published by Oxford University Press and the Society for Experimental Biology.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse distribution and reproduction in any medium, provided the original work is properly cited.