Thermophilic spore-forming spoilage organisms

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Presentation transcript:

Thermophilic spore-forming spoilage organisms

Organisms and their D-values Public health concern; Clostridium botulinum and perfringens, Bacillus cereus; D100C ~ 7-30 min Mesophilic aerobes; several Bacillus species including subtilis, licheniformis, megaterium, Alicyclobacillus acidoterrestris; D100C ~ 0.1-70 min Thermophilic aerobes; Bacillus coagulans Geobacillus stearothermophilus; D100C ~ 20-1600 min Mesophilic anaerobes; C. tyrobutyricum, butyricum, and sporogenes; D100C ~ very small (C.t and C.b) up to 80-100 min (C.s) Thermophilic anaerobes; Desulfotomaculum nigrificans and C. thermosaccharolyticum; D100C ~ 40-480 min

Endospores Great site to learn more: https://micro.cornell.edu/research/epulopiscium/bacterial-endospores

Characteristics of endospores Business end: Low moisture Proteins protecting DNA DNA repair enzymes Endospore stain

Alicyclobacillus Spore forming, gram negative, acidophilic Also grows down to pH 2.5 Heat resistant (D95C ~ 2.6 min) Found throughout juice production environment Produces guaiacol (2-methoxyphenol) Medicine, smoky, hammy, disinfectant-like

Code of Federal Regulations = CFRs