Circulating progenitor cells and coronary microvascular dysfunction: Results from the NHLBI-sponsored Women's Ischemia Syndrome Evaluation – Coronary.

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Circulating progenitor cells and coronary microvascular dysfunction: Results from the NHLBI-sponsored Women's Ischemia Syndrome Evaluation – Coronary Vascular Dysfunction Study (WISE-CVD) Girum Mekonnen, Salim S. Hayek, Puja K. Mehta, Qunna Li, Ernestine Mahar, Liping Mou, Tanya S. Kenkre, John W. Petersen, Babak Azarbal, Bruce Samuels, R. David Anderson, Tara Sedlak, Melody Zaya, Megha Agarwal, Afsaneh Haftbaradaran, Margo Minissian, Eileen Handberg, Carl J. Pepine, Christopher R. Cogle, C. Noel Bairey Merz, Edmund K. Waller, Arshed A. Quyyumi Atherosclerosis Volume 253, Pages 111-117 (October 2016) DOI: 10.1016/j.atherosclerosis.2016.08.026 Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Flow cytometric analysis of progenitor cell counts. Representative flow-cytometric analysis of peripheral blood stained with monoclonal antibodies against human fluorescein isothiocyanate (FITC)-labeledCD34, peridinin chlorophyII protein (PerCP)-labeled CD45, allophycocyanin (APC)-labeled CD133, phycoerythrin (PE)-labeled VEGFR-2 and phycoerythrin-cyanine 7 (PE-Cy7)-labeled CXCR4. First, mononuclear cells (MNC) were gated on a forward scatter (FSC) vs. side scatter (SSC) (A). Second, CD34+ cells were gated within MNC gate (B). CD45dim cells were selected within CD34+ gate. Last, CD133, VEGFR2 and CXCR4 expression was gated within CD34 + CD45dim gate (D, E, F). Atherosclerosis 2016 253, 111-117DOI: (10.1016/j.atherosclerosis.2016.08.026) Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Progenitor cells and coronary flow reserve. Relationship between log-transformed (base 2) circulating progenitor cell counts and coronary flow reserve (CFR). The scatterplots depict correlations between CFR and (A) CD34+, (B) CD34+/CD133+, (C) CD34+/CXCR4+ and (D) CD34+/VEGFR2+ cells. Pearson correlations and associated p-values are displayed. Atherosclerosis 2016 253, 111-117DOI: (10.1016/j.atherosclerosis.2016.08.026) Copyright © 2016 Elsevier Ireland Ltd Terms and Conditions