Circulating Epstein–Barr virus (EBV) in HIV-infected patients and its relation with primary brain lymphoma María Dolores Fellner, Karina Durand, Rita.

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Circulating Epstein–Barr virus (EBV) in HIV-infected patients and its relation with primary brain lymphoma María Dolores Fellner, Karina Durand, Rita Mariel Correa, Liliana Redini, Claudio Yampolsky, Antonio Colobraro, Gustavo Sevlever, Angélica R. Teyssié, Jorge Benetucci, María Alejandra Picconi International Journal of Infectious Diseases Volume 11, Issue 2, Pages 172-178 (March 2007) DOI: 10.1016/j.ijid.2006.04.001 Copyright © 2006 International Society for Infectious Diseases Terms and Conditions

Figure 1 Detection of PCR inhibitors. No inhibition was detected in DNA extracted from PBMC samples, while two DNA samples extracted from plasma exhibited inhibitors. Both samples were re-extracted, and when re-tested one showed no inhibitors so it was quantified, while the other was discarded since inhibitors could not be eliminated. (A) Detection of PCR inhibitors in DNA extracted from PBMC samples. Lanes 1–5: DNAPBMC from HIV positive patients. Lane 6: negative control. Lane 7: positive control. Lane 8: 100bp ladder. (B) Detection of PCR inhibitors in DNA extracted from plasma samples. Lane 1: 100bp ladder. Lanes 2–6: DNAplasma from HIV positive patients. Notice the presence of inhibitors in the plasma specimen run in lane 2. Lane 7: negative control. Lane 8: positive control. International Journal of Infectious Diseases 2007 11, 172-178DOI: (10.1016/j.ijid.2006.04.001) Copyright © 2006 International Society for Infectious Diseases Terms and Conditions

Figure 2 EBV detection and PBMC viral load in the study population. The limiting dilution value obtained for each patient was converted into viral copies/105 PBMC by multiplying the sensitivity of the assay (1–16 copies) by the reciprocal of the limiting dilution (RLD); e.g. if RLD=4, after multiplying by 1 and by 16, the corresponding viral load ranges between 4 and 64. ND, not detected; dotted line represents the detection level with the SQ-PCR. Bars show the median RLD value with the confidence interval range (%) for each group. International Journal of Infectious Diseases 2007 11, 172-178DOI: (10.1016/j.ijid.2006.04.001) Copyright © 2006 International Society for Infectious Diseases Terms and Conditions

Figure 3 EBV detection and load levels in HIV infected patients according to CD4+ T cell counts. The total number of patients included in this analysis is lower than that described in Figure 2 since CD4+ T cell counts were not available in 10 patients. ND, not detected; dotted line represents the detection level with the SQ-PCR. Bars show the median RLD value with the confidence interval range (%) for each group. International Journal of Infectious Diseases 2007 11, 172-178DOI: (10.1016/j.ijid.2006.04.001) Copyright © 2006 International Society for Infectious Diseases Terms and Conditions

Figure 4 Correlation between plasma-EBV and PBMC-EBV loads. A linear regression analysis of the natural logarithm of the reciprocal of the limiting dilution (RLD) values obtained from paired PBMC and plasma samples of 62 patients is represented (r=0.173; p=0.219). International Journal of Infectious Diseases 2007 11, 172-178DOI: (10.1016/j.ijid.2006.04.001) Copyright © 2006 International Society for Infectious Diseases Terms and Conditions