Heregulin, Cysteine Rich-61 and Matrix Metalloproteinase 9 Expression in Human Carotid Atherosclerotic Plaques: Relationship with Clinical Data F. Sigala,

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Heregulin, Cysteine Rich-61 and Matrix Metalloproteinase 9 Expression in Human Carotid Atherosclerotic Plaques: Relationship with Clinical Data F. Sigala, S. Georgopoulos, E. Papalambros, D. Chasiotis, G. Vourliotakis, A. Niforou, A. Kotsinas, N. Kavantzas, E. Patsouris, V.G. Gorgoulis, E. Bastounis European Journal of Vascular and Endovascular Surgery Volume 32, Issue 3, Pages 238-245 (September 2006) DOI: 10.1016/j.ejvs.2006.01.026 Copyright © 2006 Elsevier Ltd Terms and Conditions

Fig. 1 Representative immunohistochemical staining of HRG and CYR61 in complicated plaques (type VI). Staining of HRG and CYR61 was performed in serial sections to assess their topological expression. European Journal of Vascular and Endovascular Surgery 2006 32, 238-245DOI: (10.1016/j.ejvs.2006.01.026) Copyright © 2006 Elsevier Ltd Terms and Conditions

Fig. 2 Results of HRG and CYR61 expressions levels as assessed by Western blot analysis in normal carotid arteries, complicated and non-complicated atherosclerotic plaques. (A) Representative Western blot analysis results for HRG and CYR61 expression in complicated, non-complicated carotid plaques and normal carotids. Beta-actin was used to assess equal loading and normalization of HRG and CYR61 expression, respectively, for each case. (B) and (C) Box blots representing the expression levels of HRG and CYR61, respectively, in complicated, non-complicated carotid plaques and normal carotids. HRG and CYR61 expression levels were estimated by arbitrary optical integrated density units after normalization against corresponding beta-actin expression. European Journal of Vascular and Endovascular Surgery 2006 32, 238-245DOI: (10.1016/j.ejvs.2006.01.026) Copyright © 2006 Elsevier Ltd Terms and Conditions