Light Microscope Lab Chapter 2.

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Presentation transcript:

Light Microscope Lab Chapter 2

Parts of the Light Microscope Base Ocular Arm Nose piece Stage Coarse adjustment knob Obectives Fine adjustment knob Arm Stage Condenser Iris diaphragm Nosepiece Objectives Base Stage control knobs Ocular (eyepiece)

Total Magnification Objectives Ocular (eyepiece): 10X Scanning objective: 4X Low power objective: 10X High power objective: 40X – 43X Oil immersion objective: 100X Ocular (eyepiece): 10X Objective x Ocular = Total Magnification 4X x 10X = 40X 100X x 10X = 1000X

Focusing: Center slide on stage directly over light Rotate nosepiece to 4X objective Through ocular adjust light w/ diaphragm Focus with coarse then fine adjustment Rotate nosepiece to higher X as needed Use only fine adjustment knob to focus Adjust light as needed

Cell Anatomy Lab Chapter 3

p. 64 Generic Animal Cell Nucleus Nuclear membrane Nucleolus Chromatin Cytoplasm (organelles + cytosol) Mitochondrion (mitochondria) Golgi body (golgi apparatus) Golgi vesicles (secretory vesicles) Lysosome Fat vacuole Rough endoplasmic reticulum Smooth endoplasmic reticulum Ribosomes Cell membrane (plasma membrane

Cell Membrane (Plasma Membrane)

Mitosis Lab Chapter 4

1. 8. 1. 1. 5. 5. 4. 2. 4. 6. 9. 3. 3. 6. 3. 10. 7. 8. 8. 7. 4. 9. 9. 10. 11. 11. 5. 10. 11. 6. 13. 12. 13. 12. 12. 7. 2. 2. 13.

Interphase

Prophase (early)

Prophase (late)

Metaphase

Anaphase

Telophase

Daughter cells in “Interphase”