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Plant tissue culture

Definition of Plant tissue culture : Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition.

Brief History of plant tissue culture  Schelden and schwann make toti potency theory in  Successful Leaf culture by Haberlandt in  White first time established successful root culture of tomato in  Skoog and Miller demonstrated the rule of axin and cytokinin on root and shoot formation in tobacco – tissue in  Edward cocking enzymatic isolation and culture of protoplast  Murashige and Skoog introduced the medium for tobacco culture in Gottlieb Haberlandt Born28 November 1854 Mosonmagyaróvár Died30 January 1945 (aged 90) Berlin NationalityAustria FieldsBotany

Tissue culture’s purposes Production of Disease free plants True to type In short time In Small place With Low staff Anytime production

Type of cultures in Plant tissue culture Plant tissue culture Stem culture Seed cultureMeristem culture organ culture leaf culture Callus culture Bud culture Embryo culture

Stem culture Explants collection Washing Sterilizing Culturing

Bud culture Explants collection Washing Sterilizing Extraction Culturing

Embryo culture Explants collection Sterilizing Extraction Culturing

Leaf culture Explants collection washing Sterilizing Culturing

Meristem culture(Micrografiting) Explants collection washing Sterilizing Extraction

Contin.. Meristem Isolation

Continue Micro grafting

Seed culture washing Sterilizing Culturing

Gisela 5 (rootstock for sweet cheery) GF677 ( rootstock for almond) Pyro dwarf ( rootstock for pear) Rootstock production in plant tissue culture section of PBTL

Gisela 5 Its Shoot proliferation have good result in DKW media with 0.5 mg/L BAP and 0.1 mg/L IBA for the root production MS half concentration Macro elements and half concentration sucrose with 0.6 mg/L IBA is perfect.

DKW media(Dampf-Kraft-Wagen )

GF677 Its Shoot proliferation have good result in GNH media with 0.1 mg/L BAP for the root production MS half concentration Macro elements and half concentration sucrose with 0.6 mg/L IBA and 4 mg/L thiamin HCl is perfect.

GNH(Garoosi, Nezami and Haddad)

Pyro dwarf Its Shoot proliferation have good result in QL media with 0.8 mg/ BAP/L and 0.08 IBA/L for the root production MS half concentration Macro elements and half concentration sucrose without hormone

QL(Quoirin and Lepoivre)

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