DNA analysis on patients with STS deficiency

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DNA analysis on patients with STS deficiency DNA analysis on patients with STS deficiency. Panel A: Southern analysis of EcoRI-digested DNA samples from 11 patients (lanes 1 to 11) and one male control (lane 12) using an STS full-length cDNA clone. Panel B: Multiplex DNA amplification of the STS gene (STS-PCR) in 11 patients with STS deficiency (lanes 1 to 11, loaded in the same order as in A) and one male control (lane 12). Band a corresponds to an exon of the DMD gene and was used as an internal control for the amplification. Bands b and c correspond to the 3′ and the 5′ ends of the STS gene, respectively. MW = molecular weight marker. Panel C: Sequence of oligonucleotide primers for PCR amplifications of STS. F = forward primer; R = reverse primer. (From Ballabio et al.216 Used by permission of Human Genetics.) Source: Steroid Sulfatase Deficiency and X-Linked Ichthyosis, The Online Metabolic and Molecular Bases of Inherited Disease Citation: Valle D, Beaudet AL, Vogelstein B, Kinzler KW, Antonarakis SE, Ballabio A, Gibson K, Mitchell G. The Online Metabolic and Molecular Bases of Inherited Disease; 2014 Available at: https://ommbid.mhmedical.com/DownloadImage.aspx?image=/data/books/971/ch166fg10.png&sec=62649444&BookID=971&ChapterSecID=62649338&imagename= Accessed: March 02, 2018 Copyright © 2018 McGraw-Hill Education. All rights reserved