PCCSE Project Peculiar P100 Peak Picking Puzzles

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Presentation transcript:

PCCSE Project Peculiar P100 Peak Picking Puzzles LINCS PCCSE Meeting 11 Feb 2016

Data QC Pipeline Import .raw files into heavy checker during acquisition to verify optimal instrument performance Import .raw files into full DIA analysis file with precursors Import .raw files into QC DIA file to determine enrichment efficiency Manually pick correct peak range using full DIA file (“Peptide ID”) Manually refine boundaries and transitions while removing precursors (“Quant”) Add metadata into Document Grid View Export into .gct file for data analysis

“Peptide ID”

“Quant”

Peak Boundaries: KIAA1844 Can’t consistently find right peak 2 examples of this

Peak Boundaries: KIAA0701 This is integrated poorly Many more examples of this

Peak Boundaries: KIAA0701 Automatic Manual

Transition Refinement: AHNAK

Transition Refinement: BAF155

Transition Refinement: A2D Sample B1

Transition Refinement: A2D Sample B12

Transition Refinement: CDC2 Sample D8

Transition Refinement: CDC2 Sample H2