SWAFS Conference October 22-26, 2012 Scottsdale, AZ

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Presentation transcript:

SWAFS Conference October 22-26, 2012 Scottsdale, AZ Southwestern Association of Forensic Scientists SWAFS Conference October 22-26, 2012 Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Utilizing Heptafluorobutyric Anhydride (HFBA) to Confirm the Identity of Designer Drugs Benny J. Lum Broward Sheriff’s Office Crime Laboratory Fort Lauderdale, Florida SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

References SWAFS Conference 2012, Scottsdale, AZ

References Wikipedia SWAFS Conference 2012, Scottsdale, AZ

References Wikipedia McMurry, John. Organic Chemistry 5th edition (2000). Brooks/Cole. SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

References Wikipedia McMurry, John. Organic Chemistry 5th edition (2000). Brooks/Cole. Knapp, Daniel R., Handbook of Analytical Derivatization Reactions (1979). John Wiley & Sons; New York. SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

References Wikipedia McMurry, John. Organic Chemistry 5th edition (2000). Brooks/Cole. Knapp, Daniel R., Handbook of Analytical Derivatization Reactions (1979). John Wiley & Sons; New York. Lum, Benny. A Rapid Single Tube Chemical Derivatization Extraction Method to Identify Mixtures of Phenethylamines using a Finnigan Trace GC/Tempus TOF (Time of Flight) Mass Spectrometer. SWAFS Journal (2005) Vol 27, 1, p 43-52 SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Designer Drug SWAFS Conference 2012, Scottsdale, AZ

Designer Drug Designer Drugs are drugs that are created (or marketed, if they had already existed) to avoid the provisions of existing drug laws, usually by preparing analogs or derivatives of existing drugs by modifying their chemical structure to varying degrees… -source, Wikipedia SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Terminology SWAFS Conference 2012, Scottsdale, AZ

Terminology Isomers SWAFS Conference 2012, Scottsdale, AZ

Terminology Isomers 1. Constitutional- (structural isomers) SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Terminology Isomers 1. Constitutional- (structural isomers) 2. Sterioisomers Enatiomers Diasteromer Cis-trans isomers Conformers Rotamers SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Source: Wikipedia

Terminology Isomers Homolog SWAFS Conference 2012, Scottsdale, AZ

Terminology Isomers Homolog SWAFS Conference 2012, Scottsdale, AZ

Terminology Isomers Homolog Analog SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Terminology Isomers Homolog Analog Functional Analog SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Terminology Isomers Homolog Analog Functional Analog Source: Wikipedia SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Terminology Isomers Homolog Analog Functional Analog Structural Analog SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Terminology Isomers Homolog Analog Functional Analog Structural Analog Source: Wikipedia SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Terminology Isomers Homolog Analog Functional Analog Structural Analog Source: Wikipedia SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Leap of Faith SWAFS Conference 2012, Scottsdale, AZ

Leap of Faith Sciencecartoonsplus.com SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Leap of Faith Instrumentation SWAFS Conference 2012, Scottsdale, AZ

Leap of Faith Instrumentation Tuning SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Leap of Faith Instrumentation Tuning Columns selection SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Leap of Faith Instrumentation Tuning Columns selection Range of scans SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Leap of Faith Instrumentation Types of Samples Tuning Columns selection Range of scans Types of Samples SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Types of Samples

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

Heptafluorobutyric Anhydride (HFBA) SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ HFBA structure Molecular weight 410 Key piece is C4OF7 Key factor is 196 (cation) SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ HFBA-Advantages Robust Reaction at room temperature Set-up is approximately 5 minutes Stable and predictable compounds Great with mixtures Can be stored at room temperature SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ HFBA-Disadvantages Unfortunately HFBA has not work with synthetic cannabis compounds HFBA requires either primary or 2nd amine Hydroscopic reagent Odorous SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ Use of HFBA SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

Types of Samples

SWAFS Conference 2012, Scottsdale, AZ

Heptafluorobutyric Anhydride (HFBA) on cathinones SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Heptafluorobutyric Anhydride (HFBA) SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Heptafluorobutyric Anhydride (HFBA) Slide courtesy of Wikipedia SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Heptafluorobutyric Anhydride (HFBA) Slide courtesy of Wikipedia SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Heptafluorobutyric Anhydride (HFBA) SWAFS Conference 2012, Scottsdale, AZ

Heptafluorobutyric Anhydride (HFBA) SWAFS Conference 2012, Scottsdale, AZ

Samples A real typical case SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ A closer look Using HFBA on this case SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

Heptafluorobutyric Anhydride (HFBA) Distinguishing isomers 3-fluoromethcathinone (3-FMC) v 4-fluoromethcathinone (4-FMC) SWAFS Conference 2012, Scottsdale, AZ SWAFS Conference 2012, Scottsdale, AZ

Fig 5. GC and MS of 3-fluoromethcathinone

Fig. 6. GC and MS of 4-fluoromethcathinone

Fig. 8. GC peaks of parent and derivatized 4-methcathinone

Fig. 9. Mass spectra of 3-FMC v (bottom) 4-FMC, HFBA derivatized

HFBA sensitivity Case sample SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

SWAFS Conference 2012, Scottsdale, AZ

Some Interesting HFBA samples

That’s all Folks Thank you