Ursolic acid from the Chinese herb Danshen (Salvia miltiorrhiza L

Slides:



Advertisements
Similar presentations
Date of download: 5/31/2016 Copyright © The American College of Cardiology. All rights reserved. From: Red wine increases the expression of human endothelial.
Advertisements

Date of download: 9/17/2016 Copyright © The American College of Cardiology. All rights reserved. From: Red wine increases the expression of human endothelial.
Metformin prevents glucotoxicity by alleviating oxidative and ER stress–induced CD36 expression in pancreatic beta cells  Jun Sung Moon, Udayakumar Karunakaran,
Volume 36, Issue 6, Pages (June 2015)
Volume 48, Issue 6, Pages (June 2011)
IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,
Pitavastatin-induced downregulation of CCR2 and CCR5 in monocytes is associated with the arrest of cell-cycle in S phase  Masahiro Fujino, Shin-ichiro.
Simvastatin modulates chemokine and chemokine receptor expression by geranylgeranyl isoprenoid pathway in human endothelial cells and macrophages  Niels.
PTEN differentially regulates expressions of ICAM-1 and VCAM-1 through PI3K/Akt/GSK-3β/GATA-6 signaling pathways in TNF-α-activated human endothelial.
Inhibitory effect of polyphenol cyanidin on TNF-α-induced apoptosis through multiple signaling pathways in endothelial cells  Jin-Wen Xu, Katsumi Ikeda,
MicroRNA-27a/b regulates cellular cholesterol efflux, influx and esterification/hydrolysis in THP-1 macrophages  Min Zhang, Jian-Feng Wu, Wu-Jun Chen,
Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line  Aoranit Somno, Songyot.
Evidence for an exclusive association of matrix metalloproteinase-9 with dysfunctional high-density lipoprotein: A novel finding  S. Sini, D. Deepa, S.
Arterioscler Thromb Vasc Biol
Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages  B.P. Deng, Y. Zhang, Q.J. Wang, X.F. Xu, H. Zhang,
Ellagic acid inhibits oxidized LDL-mediated LOX-1 expression, ROS generation, and inflammation in human endothelial cells  Wen-Jane Lee, PhD, Hsiu-Chung.
Interferon (IFN)-Induced Protein 35 (IFI35), a Type I Interferon-Dependent Transcript, Upregulates Inflammatory Signaling Pathways by Activating Toll-Like.
NF-κB suppresses the expression of ATP-binding cassette transporter A1/G1 by regulating SREBP-2 and miR-33a in mice  Guo-Jun Zhao, Shi-Lin Tang, Yun-Cheng.
Ellagic acid inhibits oxidized LDL-mediated LOX-1 expression, ROS generation, and inflammation in human endothelial cells  Wen-Jane Lee, PhD, Hsiu-Chung.
Volume 28, Issue 4, Pages (November 2007)
Role of hypoxia-inducible factor-1 alpha in the regulation of plasminogen activator activity in rat knee joint chondrocytes  G. Zhu, Y. Tang, X. Liang,
Phosphoinositide 3-kinase inhibitors protect mouse kidney cells from cyclosporine- induced cell death  E. Sarró, O. Tornavaca, M. Plana, A. Meseguer, E.
NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes  Dr C. Lianxu, Ph.D.,
Long-term NSAID treatment directly decreases COX-2 and mPGES-1 production in the articular cartilage of patients with osteoarthritis  M.A. Álvarez-Soria,
Volume 70, Issue 5, Pages (September 2006)
Y. Higashimoto, M. Ohata, Y. Yamagata, T. Iwata, M. Masuda, T
I. Kausch, H. Jiang, B. Thode, C. Doehn, S. Krüger, D. Jocham 
Aspirin prevents resistin-induced endothelial dysfunction by modulating AMPK, ROS, and Akt/eNOS signaling  Hsiu-Chung Ou, PhD, Wen-Jane Lee, PhD, Ching-Mei.
Histone deacetylase inhibitors suppress interleukin-1β-induced nitric oxide and prostaglandin E2 production in human chondrocytes  N. Chabane, M.Sc.,
Volume 76, Issue 1, Pages (July 2009)
Vascular endothelial growth factor stimulates protein kinase CβII expression in chronic lymphocytic leukemia cells by Simon T. Abrams, Benjamin R. B. Brown,
Volume 133, Issue 3, Pages (September 2007)
Chakradhari Sharan, Ph. D. , Sunil K. Halder, Ph. D
Expression and Function of RIG-I in Oral Keratinocytes and Fibroblasts
Volume 16, Issue 4, Pages (October 2009)
Volume 78, Issue 9, Pages (November 2010)
Volume 25, Issue 4, Pages (December 2018)
Volume 75, Issue 2, Pages (January 2009)
M.H.A. Baccora, P. Cortes, C. Hassett, D.W. Taube, J. Yee 
Estrogen enhancement of SGK1 expression induced by urocortin contributes to its cardioprotection against ischemia/reperfusion insult  Binhai Cong, Jiankui.
Stefan W. Stoll, Jessica L. Johnson, Yong Li, Laure Rittié, James T
Expression and regulation of Toll-like receptor 2 by IL-1β and fibronectin fragments in human articular chondrocytes  S.-L. Su, M.S., C.-D. Tsai, Ph.D.,
An Autocrine Loop Mediates Expression of Vascular Endothelial Growth Factor in Human Dermal Microvascular Endothelial Cells  Barbara Vega-Diaz, Serge.
Effect of advanced glycation end-products on gene expression and synthesis of TNF-α and endothelial nitric oxide synthase by endothelial cells  Gloria.
Role of vitamin C and selenium in attenuation of nicotine induced oxidative stress, P53 and Bcl2 expression in adult rat spleen  Marwa A. Ahmed, K.H.
DIO2 modifies inflammatory responses in chondrocytes
Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome  Yongguang Lu, Lang Li, Hua Yan, Qiang.
Andrew J Henderson, Ruth I Connor, Kathryn L Calame  Immunity 
Volume 123, Issue 2, Pages (October 2005)
1,25-dihydroxyvitamin D3 inhibits renal interstitial myofibroblast activation by inducing hepatocyte growth factor expression  Yingjian Li, Bradley C.
Microtubule-Targeted Drugs Inhibit VEGF Receptor-2 Expression by both Transcriptional and Post-Transcriptional Mechanisms  Markus Meissner, Andreas Pinter,
Cyclooxygenase-2 Inhibitor Enhances Whereas Prostaglandin E2Inhibits the Production of Interferon-Induced Protein of 10 kDa in Epidermoid Carcinoma A431 
All-Trans-Retinoic Acid Induces Interleukin-8 via the Nuclear Factor-κB and p38 Mitogen-Activated Protein Kinase Pathways in Normal Human Keratinocytes 
Reciprocal Regulation of Thymus and Activation-Regulated Chemokine/Macrophage- Derived Chemokine Production by Interleukin (IL)-4/IL-13 and Interferon-γ.
Differential Gene Induction of Human β-Defensins (hBD-1, -2, -3, and -4) in Keratinocytes Is Inhibited by Retinoic Acid  Jürgen Harder, Ulf Meyer-Hoffert,
IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,
BMP-4 Upregulates Kit Expression in Mouse Melanoblasts prior to the Kit-Dependent Cycle of Melanogenesis  Tamihiro Kawakami, Satoko Kimura, Yoko Kawa,
The Membrane-Lytic Peptides K8L9 and Melittin Enter Cancer Cells via Receptor Endocytosis following Subcytotoxic Exposure  Masayuki Kohno, Tomohisa Horibe,
Volume 119, Issue 2, Pages (August 2000)
The impact of psychogenic stressors on oxidative stress markers and patterns of CYP2E1 expression in mice liver  Oksana Maksymchuk, Mykola Chashchyn 
Negative Regulation of Tumor Suppressor p53 by MicroRNA miR-504
Volume 70, Issue 5, Pages (September 2006)
MicroRNA-4443 regulates mast cell activation by T cell–derived microvesicles  Irit Shefler, PhD, Pazit Salamon, PhD, Francesca Levi-Schaffer, PharmD, Adam.
Suppression of VEGFR2 Expression in Human Endothelial Cells by Dimethylfumarate Treatment: Evidence for Anti-Angiogenic Action  Markus Meissner, Monika.
USP15 Negatively Regulates Nrf2 through Deubiquitination of Keap1
IL-1β induces VEGF, independently of PGE2 induction, mainly through the PI3-K/mTOR pathway in renal mesangial cells  D. Solà-Villà, M. Camacho, R. Solà,
Volume 72, Issue 2, Pages (July 2007)
Mechanisms of Hypoxic Regulation of Plasminogen Activator Inhibitor-1 Gene Expression in Keloid Fibroblasts  Qunzhou Zhang, Yidi Wu, David K. Ann, Diana.
Volume 123, Issue 2, Pages (October 2005)
Aminoglycoside Enhances the Delivery of Antisense Morpholino Oligonucleotides In Vitro and in mdx Mice  Mingxing Wang, Bo Wu, Sapana N. Shah, Peijuan.
Presentation transcript:

Ursolic acid from the Chinese herb Danshen (Salvia miltiorrhiza L Ursolic acid from the Chinese herb Danshen (Salvia miltiorrhiza L.) upregulates eNOS and downregulates Nox4 expression in human endothelial cells  Katja Steinkamp-Fenske, Larissa Bollinger, Natalie Völler, Hui Xu, Ying Yao, Rudolf Bauer, Ulrich Förstermann, Huige Li  Atherosclerosis  Volume 195, Issue 1, Pages e104-e111 (November 2007) DOI: 10.1016/j.atherosclerosis.2007.03.028 Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Total aqueous Danshen extract increases eNOS promoter activity and eNOS mRNA expression in human endothelial cells. Human EA.hy 926 endothelial cells were stably transfected with a 3.5kb human eNOS promoter fragment driving a luciferase reporter gene. These cells as well as normal EA.hy 926 cells were treated for 18h with various dilutions of total aqueous Danshen extract (stock solution corresponding to 5g raw Danshen per ml). In the transfected cells, luciferase activity was analyzed as a determinant of eNOS promoter activity (panel A). In normal EA.hy 926 cells eNOS mRNA expression was analyzed with RNase protection assays. Panel B demonstrates an original gel (performed in triplicate). The gel shows the protected bands for eNOS and for β-actin (used for normalization). Panels C and D demonstrate the concentration- and time-dependency of Danshen's effect on eNOS mRNA expression, respectively. Symbols represent mean±S.E.M., n=12–15 (*p<0.05; **p<0.01; ***p<0.001 compared with control). Atherosclerosis 2007 195, e104-e111DOI: (10.1016/j.atherosclerosis.2007.03.028) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Total aqueous Danshen extract enhances eNOS protein expression and NO production. EA.hy 926 cells were treated for 18h with aqueous Danshen extract (dilution 1:1000, stock solution corresponding to 5g raw Danshen per ml) and eNOS protein expression was analyzed with Western blot using a monoclonal anti-eNOS antibody. Panel A shows a representative blot. Panel B illustrates results of densitometric analyses. Panel C: EA.hy 926 cells were treated for 18h with various dilutions of aqueous Danshen extract. Oxidation products of NO, nitrite and nitrate, in the cell culture supernatant were assayed by NO-ozone chemiluminescence using a NO Analyzer. Columns represent mean±S.E.M., n=12–15 (*p<0.05, **p<0.01, compared with control). Atherosclerosis 2007 195, e104-e111DOI: (10.1016/j.atherosclerosis.2007.03.028) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Effects of different fractions of Danshen on human eNOS promoter activity and eNOS mRNA expression. Human EA.hy 926 endothelial cells were stably transfected with a 3.5kb human eNOS promoter fragment driving a luciferase reporter gene. Cells were treated for 18h with dichloromethane, methanol and water extracts of Danshen (100μg/ml each). Luciferase activity was analyzed as a determinant of eNOS promoter activity. eNOS mRNA expression was analyzed with RNase protection assays. Columns represent mean±S.E.M., n=9–12 (*p<0.05, **p<0.01 vs. untreated cells). Atherosclerosis 2007 195, e104-e111DOI: (10.1016/j.atherosclerosis.2007.03.028) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 4 Ursolic acid enhances eNOS mRNA expression, eNOS protein expression and NO production. Human EA.hy 926 cells (panel A) or HUVEC (panel B) were treated with the alcohol-soluble Danshen constituents baicalin or ursolic acid for 18h and eNOS mRNA expression was analyzed with quantitative Real-Time RT-PCR. Panel C: EA.hy 926 cells were treated for 18h with ursolic acid (10μM) and eNOS protein expression was analyzed with Western blot using a monoclonal anti-eNOS antibody. Panel D: Human EA.hy 926 cells were treated with ursolic acid (10μM) for 18h. Bioactive NO from EA.hy 926 cells was bioassayed using guanylyl cyclase-containing RFL-6 reporter cells. cGMP content in RFL-6 cells was determined with radioimmunoassay. Columns represent mean±S.E.M., n=9–18 (**p<0.01 compared with control). Atherosclerosis 2007 195, e104-e111DOI: (10.1016/j.atherosclerosis.2007.03.028) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 5 Ursolic acid downregulates Nox4 expression and reduces ROS production. Human EA.hy 926 cells were treated with 10μM ursolic acid for 18h and mRNA expression of NADPH oxidase subunits Nox4 and p22phox was analyzed with quantitative Real-Time RT-PCR (panel A). Panel B: EA.hy 926 cells, with or without apocynin pre-treatment (300μM, 48h), were treated with 10μM ursolic acid for 18h. ROS production was measured with L-012-derived chemiluminescence. Columns represent mean±S.E.M., n=9–15 (**p<0.01; n.s., not significant). Atherosclerosis 2007 195, e104-e111DOI: (10.1016/j.atherosclerosis.2007.03.028) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions