To Rerack the Spectrum Library, Three programs will be run.

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Presentation transcript:

To Rerack the Spectrum Library, Three programs will be run. SpecReRack1 Moves 50 ul to Storage plate ABGene0781_384Storage Spec Aliquot Dispenses 10 ul from storage plate to three screening plates ABGene1055_384 Dispenses 3 ul into Plate with 72 ul of DMSO for diluted plate SpecRerack 70 ul Moves the remaining Chemical Library to Storage plate.

Turn On instrument and hotel. Check Deck of instrument and remove all tip boxes and plates ect. Open Biomek Software Be sure gripper UP Home all axes.

Thaw libraries (about 1 hour) Centrifuge briefly. Open SpecReRack1 Set up Deck (red arrow) Hit start (Blue Arrow)

Check deck again All errors in deck set up will crash the robot. So check Again! Run program

Close program Remove used tips Spin Storage plate to remove bubbles Open Spec Aliquot Set up deck

Run Spec Aliquots program Check deck again when prompted

Run SpecRerack 70 ul Quick spin for all plates to put all chemicals at bottom of well.