Preferential Expansion of Vγ9-JγP/Vδ2-Jδ3 γδ T Cells in Nasal T-Cell Lymphoma and Chronic Active Epstein-Barr Virus Infection Michiko K. Oyoshi, Hiroshi.

Slides:

Advertisements

Similar presentations

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

Advertisements

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis Philippe Szankasi, David.

Long-Term Complete Regression of Nodal Marginal Zone Lymphoma Transformed Into Diffuse Large B-Cell Lymphoma With Highly Active Antiretroviral Therapy.

Amy M. Dworkin, Stephanie Y. Tseng, Dawn C. Allain, O

Hepatosplenic and Subcutaneous Panniculitis-Like γ/δ T Cell Lymphomas Are Derived from Different Vδ Subsets of γ/δ T Lymphocytes Grzegorz K. Przybylski,

Effectiveness of Capillary Electrophoresis Using Fluorescent-Labeled Primers in Detecting T-Cell Receptor γ Gene Rearrangements Timothy C. Greiner, Ronald.

Epstein-Barr Virus Strains With Latent Membrane Protein-1 Deletions: Prevalence in the Italian Population and High Association With Human Immunodeficiency.

Molecular Diagnosis of Epstein-Barr Virus-Related Diseases

The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large.

A Rapid Polymerase Chain Reaction-Based Screening Method for Identification of All Expanded Alleles of the Fragile X (FMR1) Gene in Newborn and High-Risk.

Effectiveness of Capillary Electrophoresis Using Fluorescent-Labeled Primers in Detecting T-Cell Receptor γ Gene Rearrangements Timothy C. Greiner, Ronald.

Detection of Clonally Restricted Immunoglobulin Heavy Chain Gene Rearrangements in Normal and Lesional Skin Minakshi Nihal, Debra Mikkola, Gary S. Wood

Analysis of T-Cell Clonality Using Laser Capture Microdissection and High-Resolution Microcapillary Electrophoresis Evgeny Yakirevich, Cynthia L. Jackson,

Comparison of BIOMED-2 Versus Laboratory-Developed Polymerase Chain Reaction Assays for Detecting T-Cell Receptor-γ Gene Rearrangements Keyur P. Patel,

Expansion of an FMR1 Grey-Zone Allele to a Full Mutation in Two Generations Isabel Fernandez-Carvajal, Blanca Lopez Posadas, Ruiqin Pan, Christopher Raske,

Isothermal Multiple Displacement Amplification

IgH PCR of Zinc Formalin-Fixed, Paraffin-Embedded Non-Lymphomatous Gastric Samples Produces Artifactual “Clonal” Bands Not Observed in Paired Tissues.

by Jean-Michel Cayuela, Betty Gardie, and François Sigaux

Simultaneous Detection and Quantification of Mitochondrial DNA Deletion(s), Depletion, and Over-Replication in Patients with Mitochondrial Disease Ren-Kui.

B-Cell Clonality Determination Using an Immunoglobulin κ Light Chain Polymerase Chain Reaction Method Reetesh K. Pai, Artemis E. Chakerian, John M. Binder,

Association of Clinical Status of Follicular Lymphoma Patients after Autologous Stem Cell Transplant and Quantitative Assessment of Lymphoma in Blood.

Molecular Diagnostic Approach to Non-Hodgkin's Lymphoma

Molecular Diagnosis in Ewing Family Tumors

Undifferentiated Small Round Cell Sarcomas with Rare EWS Gene Fusions

Sequencing of t(2;7) Translocations Reveals a Consistent Breakpoint Linking CDK6 to the IGK Locus in Indolent B-Cell Neoplasia Edward P.K. Parker, Reiner.

Thomas W. Prior, Scott J. Bridgeman

Rapid Detection of Clonal T-Cell Receptor-β Gene Rearrangements in T-Cell Lymphomas Using the LightCycler-Polymerase Chain Reaction with DNA Melting Curve.

Hepatosplenic and Subcutaneous Panniculitis-Like γ/δ T Cell Lymphomas Are Derived from Different Vδ Subsets of γ/δ T Lymphocytes Grzegorz K. Przybylski,

Brenton T. Tan, Roger A. Warnke, Daniel A. Arber

SeqSharp The Journal of Molecular Diagnostics

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis Philippe Szankasi, David.

Volume 10, Issue 5, Pages (May 1999)

Catherine E. Keegan, Anthony A. Killeen

Rapid Detection of TEM-Type Extended-Spectrum β-Lactamase (ESBL) Mutations Using Lights-On/Lights-Off Probes with Single-Stranded DNA Amplification Kenneth.

Screening for Expanded Alleles of the FMR1 Gene in Blood Spots from Newborn Males in a Spanish Population Isabel Fernandez-Carvajal, Paulina Walichiewicz,

Inconspicuous Insertion 22;12 in Myxoid/Round Cell Liposarcoma Accompanied by the Secondary Structural Abnormality der(16)t(1;16) Nathan C. Birch, Cristina.

Diagnostic Utility of Molecular Investigation in Extraskeletal Myxoid Chondrosarcoma Stefania Benini, Stefania Cocchi, Gabriella Gamberi, Giovanna Magagnoli,

Comparison of QIAsymphony Automated and QIAamp Manual DNA Extraction Systems for Measuring Epstein-Barr Virus DNA Load in Whole Blood Using Real-Time.

Validation and Clinical Application of a Locus-Specific Polymerase Chain Reaction- and Minisequencing-Based Assay for Congenital Adrenal Hyperplasia (21-Hydroxylase.

Syed Hussain Askree, Lawrence N

Suzanne M. Morgan, Elizabeth Hodges, Tracey J

Laurent Gouya Journal of Investigative Dermatology

Volume 7, Issue 1, Pages (July 2010)

Rare Sequence Variation in the Genome Flanking a Short Tandem Repeat Locus Can Lead to a Question of “Nonmaternity” Anne Deucher, Tsoyu Chiang, Iris.

Immunoglobulin Heavy Chain Gene Analysis in Lymphomas

Presence of Epstein–Barr Virus in Langerhans Cells of CTCL Lesions

Allison M. Cushman-Vokoun, Solomon Connealy, Timothy C. Greiner

N. Scott Reading, Stephen D. Jenson, Jeffrey K. Smith, Megan S

Olga A. Gra, Julia V. Sidorova, Eugene A. Nikitin, Alexander Y

Polymerase Chain Reaction Detection of Kaposi's Sarcoma-Associated Herpesvirus- Optimized Protocols and Their Application to Myeloma Langxing Pan, Laura.

Ye Bang-Ce, Chu Xiaohe, Fan Ye, Li Songyang, Yin Bincheng, Zuo Peng

Analysis of hMLH1 and hMSH2 Gene Dosage Alterations in Hereditary Nonpolyposis Colorectal Cancer Patients by Novel Methods Linnea M. Baudhuin, Ming Mai,

An Allele-Specific RT-PCR Assay to Detect Type A Mutation of the Nucleophosmin-1 Gene in Acute Myeloid Leukemia Tiziana Ottone, Emanuele Ammatuna, Serena.

Amplification Refractory Mutation System, a Highly Sensitive and Simple Polymerase Chain Reaction Assay, for the Detection of JAK2 V617F Mutation in Chronic.

Amplification and Overexpression of the EMS 1 Oncogene, a Possible Prognostic Marker, in Human Hepatocellular Carcinoma Bao-Zhu Yuan, Xiaoling Zhou,

Novel Polymorphism in the FMR1 Gene Resulting in a “Pseudodeletion” of FMR1 in a Commonly Used Fragile X Assay Thomas M. Daly, Arash Rafii, Rick A. Martin,

T Cell Receptor γ-Chain Gene Polymerase Chain Reaction to Diagnose Central Nervous System Involvement by Cutaneous T Cell Lymphoma Robert Taylor, Jo-Anne.

The Distribution of Gene Segments in T-Cell Receptor γ Gene Rearrangements Demonstrates the Need for Multiple Primer Sets Lyle C. Lawnicki, Ronald J.

Rapid Polymerase Chain Reaction-Based Detection of Epidermal Growth Factor Receptor Gene Mutations in Lung Adenocarcinomas Qiulu Pan, William Pao, Marc.

S. Alexiou-Daniel, A. Stylianakis, A. Papoutsi, I. Zorbas, A. Papa, A

Sensitive Detection of Polyalanine Expansions in PHOX2B by Polymerase Chain Reaction Using Bisulfite-Converted DNA Hidekazu Horiuchi, Ayako Sasaki, Motoki.

Katie Ridd, Siegrid Yu, Boris C. Bastian

Kathleen M. Murphy, Tanya Geiger, Michael J. Hafez, James R

Xiangfeng Cui, Helen Feiner, Honghua Li

Validation of Roche LightCycler Epstein-Barr Virus Quantification Reagents in a Clinical Laboratory Setting Margaret L. Gulley, Hongxin Fan, Sandra H.

BIOMED-2 Multiplex Immunoglobulin/T-Cell Receptor Polymerase Chain Reaction Protocols Can Reliably Replace Southern Blot Analysis in Routine Clonality.

Characterization of a Recurrent Novel Large Duplication in the Cystic Fibrosis Transmembrane Conductance Regulator Gene Feras M. Hantash, Joy B. Redman,

by Honglin Chen, Paul Smith, Richard F. Ambinder, and S. Diane Hayward

Fang Wang, Yunfeng Wang, Jie Ding, Jiyun Yang Kidney International

Quantification of bcl-2/JH Fusion Sequences and a Control Gene by Multiplex Real- Time PCR Coupled with Automated Amplicon Sizing by Capillary Electrophoresis

Presentation transcript:

Preferential Expansion of Vγ9-JγP/Vδ2-Jδ3 γδ T Cells in Nasal T-Cell Lymphoma and Chronic Active Epstein-Barr Virus Infection Michiko K. Oyoshi, Hiroshi Nagata, Nobuhiro Kimura, Yu Zhang, Ayako Demachi, Toshiro Hara, Hirokazu Kanegane, Yoshinobu Matsuo, Tomohiro Yamaguchi, Tomohiro Morio, Atsuyoshi Hirano, Norio Shimizu, Kohtaro Yamamoto The American Journal of Pathology Volume 162, Issue 5, Pages 1629-1638 (May 2003) DOI: 10.1016/S0002-9440(10)64297-6 Copyright © 2003 American Society for Investigative Pathology Terms and Conditions

Figure 1 Schematic organization of the human TCRδ and TCRγ loci. The TCRδ locus is located entirely within the TCRα locus between the TCRVα and the TCRJα gene segments. TCRVγ genes are divided into four subfamilies (I to IV) based on sequence similarity. Each Vγ gene is assigned to its subgroup as indicated. The solid boxes are gene segments used in SNT cell lines. The American Journal of Pathology 2003 162, 1629-1638DOI: (10.1016/S0002-9440(10)64297-6) Copyright © 2003 American Society for Investigative Pathology Terms and Conditions

Figure 2 Clonality of cells assessed using EBV terminal repeats. Southern blot analysis of the number of EBV-TRs as evidence for monoclonal expansion of EBV-positive cells in SNT cell lines. Lane 1, SNT-8; lane 2, SNT-13, and lane 3, SNT-15 cells. B95-8 cells (B) and Raji cells (R) were used as controls for cells with productive EBV expansion and monoclonal EBV replication, respectively. The American Journal of Pathology 2003 162, 1629-1638DOI: (10.1016/S0002-9440(10)64297-6) Copyright © 2003 American Society for Investigative Pathology Terms and Conditions

Figure 3 Southern blot analysis for TCR genes. Shown are the hybridizations with the Jγ1 probe (A), the Jδ1 probe (B), and the Jδ3 probe (C). Southern blot analysis shows rearrangement of the Jγ1, Jδ1, and Jδ3 genes in all SNT cell lines. Human placental DNA was used as a germline configuration (c); 8, SNT-8; 13, SNT-13; 15, SNT-15 cells. The American Journal of Pathology 2003 162, 1629-1638DOI: (10.1016/S0002-9440(10)64297-6) Copyright © 2003 American Society for Investigative Pathology Terms and Conditions

Figure 4 PCR analysis for the junction of Vδ and Jδ genes. PCR analysis shows the presence of the Vδ2-Jδ3 TCR gene rearrangement in the three SNT cell lines. Only the combination of a L-Vδ2 sense primer and a Jδ3 anti-sense primer amplified ∼500-bp PCR products identified as Vδ2-Jδ3. Lane 1, SNT-8; lane 2, SNT-13; lane 3, SNT-15 cells. No signal was detected in EBV-negative γδ T-cell lines; lane 4, Molt-14; lane 5, Peer; lane 6, Loucy. The American Journal of Pathology 2003 162, 1629-1638DOI: (10.1016/S0002-9440(10)64297-6) Copyright © 2003 American Society for Investigative Pathology Terms and Conditions