Cryo-em Electron microscopy (EM) has become an extremely popular method for the ultrastructural study of macromolecules, cells and tissues. An aqueous.

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Presentation transcript:

cryo-em Electron microscopy (EM) has become an extremely popular method for the ultrastructural study of macromolecules, cells and tissues. An aqueous biological sample is frozen rapidly and irradiated with a beam of electrons. A detector senses how the electrons are scattered and a computer reconstructs the 3D-shape of the molecule.

Why you need this service? You have small amount of materials; The material is hard to crystallize; You are looking into the molecules in atomic details; It is a large biomolecular complex (>150 kDa, up to 2000 Å); You want to observe the real "native state" structure.