Example of the storage location of the sample folder Attention The demonstration uses sample data installed on your PC along with AIST-MeRAM. So, even if you don’t have any data at hand, you can start risk assessment with AIST-MeRAM. As shown below, sample data are normally stored in the folder of your choice. e.g. Computer -> Local Disk (C:) -> Program Files (x86) -> AIST-MeRAM_ENG -> SAMPLEDATA Example of the storage location of the sample folder

Demonstration of Initial Assessment Using AIST-MeRAM 　Risk Assessment by Initial Mode Get a sense of what you can do with AIST-MeRAM Practice: Repeat the steps following the handouts and sample data

Initial assessment Exposure Assessment Hazard Assessment Risk 2012/8/31 Initial assessment Exposure Assessment Hazard Assessment Risk Assessment Risk Management Screening-level risk assessment TierⅠof primary risk assessment

Initial assessment (exposure assessment) Step 1/7 Select “Initial Assessment” Select “Create New File” Select “Exposure Assessment” in Assessment Steps.

Initial assessment (exposure assessment) Step 2/7 Select the sample substance for initial assessment.

Initial assessment (exposure assessment) Step 3/7 Select an assessment method for initial assessment

Initial assessment (exposure assessment) Step 4/7 Upload a list of CSCL notification information for initial assessment. The list is normally stored in “Programs Files (X86)” -> “AIST-MeRAM_ENG” -> “SAMPLE” on C drive.

Initial assessment (exposure assessment) Step 5/7 List of uploaded information

Initial assessment (exposure assessment) Step 6/7 Confirm assessment setting.

Initial assessment (exposure assessment) Step 7/7 Exposure assessment result for 4-Nonylphenol Total emission amount and exposure class Emission amount by life cycle stage & usage

Initial assessment Exposure Assessment Hazard Assessment Risk 2012/8/31 Initial assessment Exposure Assessment Hazard Assessment Risk Assessment Risk Management Screening-level risk assessment TierⅠof primary risk assessment

Initial assessment (exposure assessment) Step 1/9 Select “Reassess” in the result sheet of the previous exposure assessment. As you go back to the “Confirm” window, select “Assessment Model” in the left pane.

Initial assessment (exposure assessment) Step 2/9 As you go back to this window, change the assessment model to “Tier Ⅰof Primary Risk Assessment”.

Initial assessment (exposure assessment) Step 3/9 Select methods for estimating the emission amount.

Initial assessment (exposure assessment) Step 4/9 Upload a list of CSCL notification information for initial assessment. The list is normally stored in “Programs Files (X86)” -> “AIST-MeRAM_ENG” -> “SAMPLE” on C drive.

Initial assessment (exposure assessment) Step 5/9 List of uploaded information

Initial assessment (exposure assessment) Step 6/9 Enter physical property data

Initial assessment (exposure assessment) Step 7/9 Select the environmental data for the exposure scenarios.

Initial assessment (exposure assessment) Step 8/9

Initial assessment (exposure assessment) Step 9/9 Estimated exposure concentrations (PEC) by life stage and use category Data settings registered/entered by users Emission factors and default environmental parameters

Initial assessment Exposure Assessment Hazard Assessment Risk 2012/8/31 Initial assessment Exposure Assessment Hazard Assessment Risk Assessment Risk Management

Initial assessment (hazard assessment) Step 1/8 2012/8/31 Initial assessment (hazard assessment) Step 1/8 Go back to the Assessment Steps window by clicking the red square button. Select “Hazard Assessment”. Select “Create New File”

Initial assessment (exposure assessment) Step 2/8 Select the sample substance for initial assessment.

Initial assessment (hazard assessment) Step 3/8 Select toxicity test databases used for hazard assessment.

Initial assessment (hazard assessment) Step 4/8 Description of the assessment methods.

Initial assessment (hazard assessment) Step 5/8 Select a reliability level of toxicity data for hazard assessment.

Initial assessment (hazard assessment) Step 6/8 UFs are automatically determined by the method you select below, based on the availability of toxicity data.

Initial assessment (hazard assessment) Step 7/8 Check data/output settings. Hazard assessment using hazard quotient Hazard assessment using CSCL screening

Initial assessment (hazard assessment) Step 8/8 Hazard assessment using hazard quotient method Hazard assessment using CSCL screening method PNEC and uncertainty factor Hazard class, PNEC, and uncertainty Toxicity data Toxicity data

Initial assessment (risk assessment ) 2012/8/31 Initial assessment (risk assessment ) Hazard Assessment Exposure Assessment Risk Assessment Risk Management

Initial assessment (risk assessment) Step 1/6 Select “Risk Assessment” in Assessment Steps. Go back to the Assessment Steps window by clicking the red square button. Select “Create New File”

Initial assessment (risk assessment) Step 2/6 List of possible risk assessment combinations (hazard and exposure assessment results) is automatically displayed by the tool.

Initial assessment (risk assessment) Step 3/6 Risk assessment results for the sample substance are displayed.

Initial assessment (risk assessment) Step 4/6 Result shown as a Priority Assessment Chemical Substance Priority matrix of CSCL

Initial assessment (risk assessment) Step 5/6 Risk assessment results shown as MOE MOE>UF No risk MOE<=UF Have risk MOE>UF MOE<UF

Initial assessment (risk assessment) Step 6/6 Result sheet of risk assessment by combination of “hazard quotient” and “risk assessmentⅠunder CSCL”. Result shown as MOE MOE>UF No risk MOE<=UF Have risk MOE>UF MOE<UF

Initial assessment (risk management) Hazard Assessment Exposure Assessment Risk Assessment Risk Management

Initial assessment (risk management) 1/3 Select “Risk Management” in Assessment Steps. Go back to the Assessment Steps window by clicking the red square button.

Initial assessment (risk management) 2/3 Select a risk assessment result file for risk management Select “Risk management options” for risk management

Initial assessment (risk management) 3/3 Finish Yes No Finish

Example of the storage location of the sample folder Attention The demonstration uses sample data installed on your PC along with AIST-MeRAM. So, even if you don’t have any data at hand, you can start risk assessment with AIST-MeRAM. As shown below, sample data are normally stored in the folder of your choice. e.g. Computer -> Local Disk (C:) -> Program Files (x86) -> AIST-MeRAM_ENG -> SAMPLEDATA Example of the storage location of the sample folder