1-4 Tools and Procedures Key Concepts: Vocabulary: What measurement system do most scientists use? How are light microscopes and electron microscopes similar? How are they different? Vocabulary: Metric system Microscope Compound light microscope Electron microscope Cell culture
Microscopes Two main types: Light Microscopes Electron Microscopes
EYEPIECE COARSE ADJUSTMENT FINE ADJUSTMENT ARM LOW-POWER OBJECTIVE ARM HIGH-POWER OBJECTIVE STAGE STAGECLIPS FINE ADJUSTMENT STAGECLIPS BASE IRIS MIRROR COARSE ADJUSTMENT LAMP DIAPHRAGM
MICROSCOPE NOTES COMPOUND LIGHT MICROSCOPE: most common scope Advantages: get higher magnification than with a dissecting scope. Drawbacks: the light comes from below so sample must be very thin so you can see Usually view dead things with this microscope. Limit of resolution: if you magnify beyond this point, the object will be blurry.
DISECTING MICROSCOPE: used to view dissection or small live (sleeping) animals such as fruit flies. Advantages: can view samples that are alive; large field of view Drawbacks: magnification is not as high as other microscopes.
SCANNING ELECTRON MICROSCOPE (SEM): used to see very small “stuff” Advantages: can study very small objects such as viruses and individual molecules; can magnify things 1000x that of a light microscope. Drawbacks: object must be dead; also very expensive!
Face of an ant! IMAGES PINHEAD