Expression of FcγRIII (CD16) on human peripheral blood eosinophils increases in allergic conditions Francis Davoine, MSc, Sophie Lavigne, MSc, Jamila.

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Presentation transcript:

Expression of FcγRIII (CD16) on human peripheral blood eosinophils increases in allergic conditions Francis Davoine, MSc, Sophie Lavigne, MSc, Jamila Chakir, PhD, Claudine Ferland, BSc, Marie-Ève Boulay, BSc, Michel Laviolette, MD Journal of Allergy and Clinical Immunology Volume 109, Issue 3, Pages 463-469 (March 2002) DOI: 10.1067/mai.2002.121952 Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 1 Eosinophils and neutrophils express CD16 at similar fluorescence levels. This is a typical example of flow cytometry analysis of blood leukocytes obtained from an asthmatic subject. A, Isotype staining of leukocytes. Eosinophils (a) were discriminated from other leukocytes (b) through use of autofluorescence and dSS characteristics of eosinophils after fixation and permeabilization and CD49 positivity. B, CD49 immunostaining. All eosinophils (a) were positive for CD49. Some cells with low dSS (c) , probably lymphocytes, were also positive for CD49. C, CD16 immunostaining. Some eosinophils expressed CD16 (d) . The intensity (fluorescence level) of CD16 expression of CD16+ eosinophils was similar to that of neutrophils (e) . Other leukocytes with low dSS did not express CD16. Note that because of their smaller size and weaker autofluorescence in comparison with granulocytes, the lymphocyte population was not entirely captured in this dot-plot histogram. Journal of Allergy and Clinical Immunology 2002 109, 463-469DOI: (10.1067/mai.2002.121952) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 2 Eosinophils from subjects with allergic rhinitis and allergic asthma express more CD16 than those from normal controls and nonallergic asthmatic individuals. For each individual, the dots represent the percentage of CD16+ eosinophils and the lines represent the median value. Columns with different letters are statistically different (a ≠ b; P < .05 by Kruskal-Wallis test). Journal of Allergy and Clinical Immunology 2002 109, 463-469DOI: (10.1067/mai.2002.121952) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 3 CD16+ eosinophils express less CD11b (A ) and IL-12p35 (B ) but more IL-5 receptor (C ) than do CD16− eosinophils. The horizontal lines represent the means. Values for each individual are linked by a line. Journal of Allergy and Clinical Immunology 2002 109, 463-469DOI: (10.1067/mai.2002.121952) Copyright © 2002 Mosby, Inc. Terms and Conditions