Volume 13, Issue 6, Pages (June 2006)

Slides:

Advertisements

Similar presentations

Volume 11, Issue 11, Pages (November 2004)

Advertisements

Volume 23, Issue 4, Pages (April 2016)

Volume 23, Issue 4, Pages (April 2016)

The Genetic Basis of White Tigers

Volume 23, Issue 8, Pages (August 2016)

Volume 19, Issue 9, Pages (September 2012)

Prediction of virological response by pretreatment hepatitis B virus reverse transcriptase quasispecies heterogeneity: the advantage of using next-generation.

Volume 16, Issue 10, Pages (October 2009)

Foundations for Directed Alkaloid Biosynthesis

Volume 20, Issue 8, Pages (August 2013)

Biosynthesis of the Antitumor Agent Chartreusin Involves the Oxidative Rearrangement of an Anthracyclic Polyketide Zhongli Xu, Kathrin Jakobi, Katrin.

Volume 15, Issue 7, Pages (July 2008)

Ribosome Evolution for Two Artificial Amino Acids in E. coli

Volume 15, Issue 2, Pages (February 2008)

Adding Specificity to Artificial Transcription Activators

Volume 20, Issue 6, Pages (June 2013)

Volume 13, Issue 4, Pages (April 2006)

Volume 15, Issue 9, Pages (September 2008)

Volume 10, Issue 9, Pages (September 2003)

Mechanism of Thioesterase-Catalyzed Chain Release in the Biosynthesis of the Polyether Antibiotic Nanchangmycin Tiangang Liu, Xin Lin, Xiufen Zhou, Zixin.

Volume 23, Issue 4, Pages (April 2016)

Volume 19, Issue 2, Pages (February 2012)

Volume 10, Issue 5, Pages (May 2003)

Elucidation of the Biosynthetic Gene Cluster and the Post-PKS Modification Mechanism for Fostriecin in Streptomyces pulveraceus Rixiang Kong, Xuejiao.

Volume 19, Issue 3, Pages (March 2012)

Yit-Heng Chooi, Ralph Cacho, Yi Tang Chemistry & Biology

Volume 23, Issue 3, Pages (March 2016)

Volume 14, Issue 2, Pages (February 2007)

Volume 20, Issue 12, Pages (December 2013)

Yi-Ling Du, Doralyn S. Dalisay, Raymond J. Andersen, Katherine S. Ryan

Insights into Bacterial 6-Methylsalicylic Acid Synthase and Its Engineering to Orsellinic Acid Synthase for Spirotetronate Generation Wei Ding, Chun.

Volume 18, Issue 5, Pages (May 2011)

Volume 18, Issue 10, Pages (October 2011)

Ribosome Evolution for Two Artificial Amino Acids in E. coli

Volume 10, Issue 11, Pages (November 2003)

Volume 17, Issue 1, Pages (January 2010)

Volume 14, Issue 2, Pages (February 2007)

Volume 10, Issue 5, Pages (May 2003)

In Vivo Biocombinatorial Synthesis of Lipopeptides by COM Domain-Mediated Reprogramming of the Surfactin Biosynthetic Complex Claudia Chiocchini, Uwe.

Evidence for a Protein-Protein Interaction Motif on an Acyl Carrier Protein Domain from a Modular Polyketide Synthase Kira J. Weissman, Hui Hong, Bojana.

Volume 15, Issue 5, Pages (May 2008)

Volume 12, Issue 11, Pages (November 2005)

Foundations for Directed Alkaloid Biosynthesis

In Vivo Characterization of Nonribosomal Peptide Synthetases NocA and NocB in the Biosynthesis of Nocardicin A Jeanne M. Davidsen, Craig A. Townsend

Volume 11, Issue 11, Pages (November 2004)

Volume 17, Issue 4, Pages (April 2010)

Markerless Mutations in the Myxothiazol Biosynthetic Gene Cluster

Volume 15, Issue 6, Pages (June 2008)

Volume 8, Issue 3, Pages (March 2001)

Characterization of the Biosynthetic Gene Cluster for Benzoxazole Antibiotics A33853 Reveals Unusual Assembly Logic Meinan Lv, Junfeng Zhao, Zixin Deng,

Volume 13, Issue 3, Pages (March 2006)

Volume 11, Issue 3, Pages (March 2004)

Volume 18, Issue 12, Pages (December 2011)

Volume 16, Issue 2, Pages (February 2009)

Volume 12, Issue 2, Pages (February 2005)

Volume 24, Issue 2, Pages (February 2017)

Leinamycin Biosynthesis Revealing Unprecedented Architectural Complexity for a Hybrid Polyketide Synthase and Nonribosomal Peptide Synthetase Gong-Li.

Volume 10, Issue 4, Pages (April 2003)

Volume 21, Issue 9, Pages (September 2014)

Volume 19, Issue 3, Pages (March 2012)

Volume 18, Issue 1, Pages (January 2011)

Volume 14, Issue 6, Pages (June 2007)

Volume 13, Issue 6, Pages (June 2006)

Volume 13, Issue 7, Pages (July 2006)

Cloning, Heterologous Expression, and Characterization of the Gene Cluster Required for Gougerotin Biosynthesis Guoqing Niu, Lei Li, Junhong Wei, Huarong.

Volume 15, Issue 7, Pages (July 2008)

Volume 12, Issue 3, Pages (March 2005)

Volume 12, Issue 3, Pages (March 2005)

Nonribosomal Biosynthesis of Fusaricidins by Paenibacillus polymyxa PKB1 Involves Direct Activation of a d-Amino Acid Jingru Li, Susan E. Jensen Chemistry.

Volume 21, Issue 9, Pages (September 2014)

Presentation transcript:

Volume 13, Issue 6, Pages 575-585 (June 2006) Genetic Characterization of the Chlorothricin Gene Cluster as a Model for Spirotetronate Antibiotic Biosynthesis Xin-Ying Jia, Zhen-Hua Tian, Lei Shao, Xu-Dong Qu, Qun-Fei Zhao, Jian Tang, Gong-Li Tang, Wen Liu Chemistry & Biology Volume 13, Issue 6, Pages 575-585 (June 2006) DOI: 10.1016/j.chembiol.2006.03.008 Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 1 Structures of Chlorothricin, Deschloro-Chlorothricin, Desmethylsalicycloyl Chlorothricin, Kijanimicin, and Tetrocarcin A Chemistry & Biology 2006 13, 575-585DOI: (10.1016/j.chembiol.2006.03.008) Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 2 HPLC Analysis of CHL Production by S. antibioticus Wild-Type and Recombinant Strains (I) An authentic standard, (II) TL1001 (ΔNGDH), (III) TL1002 (ΔPKS-P1), (IV) TL1003 (ΔPKS-P2), (V) TL1006 (ΔchlD3), (VI) TL1010 (chlD3:ermE∗), (VII) wild-type, (VIII) TL1005 (ΔchlC7), (IX) TL1009 (chlC7:ermE∗), (X) TL1007 (ΔchlD4), (XI) TL1011 (chlD4:ermE∗), and (XII) TL1004 (Δorf(-1)). The asterisk and dark dot indicate CHL and Deschloro-CHL, respectively. Chemistry & Biology 2006 13, 575-585DOI: (10.1016/j.chembiol.2006.03.008) Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 3 Restriction Map and Genetic Organization of the CHL Biosynthetic Gene Cluster (A) Restriction map of the 122 kb DNA region from S. antibioticus DSM 40725 as represented by seven overlapping cosmid clones. “B,” BamHI. Solid black bars indicate the sequenced DNA region; solid quadrates indicate the probe loci. (B) Genetic organization of the CHL biosynthetic gene cluster. Proposed functions of individual orfs are shade coded and summarized in Table 1. Chemistry & Biology 2006 13, 575-585DOI: (10.1016/j.chembiol.2006.03.008) Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 4 Proposed Biosynthetic Pathways (A–D) Pathways for (A) deoxysugar olivose, (B) 2-methoxy-5-chloro-6 methylsalicyclic acid, (C) 3-carbon unit enoylpyruvate, and (D) chlorothricolide. A model for the assembly of CHL is also shown. Chemistry & Biology 2006 13, 575-585DOI: (10.1016/j.chembiol.2006.03.008) Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 5 Bioassay and HPLC Analysis of CHL and DM-CHL (A) Determination of antibacterial activities of CHL and DM-CHL against B. subtilis. Each sample was dissolved in 10 μl methanol and then added to the round filter paper. (1) 10 μl methanol (negative control), (2) 10 μg CHL, (3) 1 μg CHL, (4) 10 μg DM-CHL, and (5) 1 μg DM-CHL. (B) HPLC analysis of CHL or DM-CHL production. (I) S. antibioticus DSM 40725, (II) TL1008 (ΔchlB6), (III) TL1012 (ΔchlB1), and (IV) TL1013 (chlB1:ermE∗). The asterisk and triangle indicate CHL and DM-CHL, respectively. Chemistry & Biology 2006 13, 575-585DOI: (10.1016/j.chembiol.2006.03.008) Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 6 Sequence Analysis of the ChlA1–A6 PKSs (A) Alignments of the conserved motifs of AT domains from CHL PKSs. Asterisks indicate the conserved amino acid residues that determine the substrate specificity, and ATs that activate methylmalonyl-CoA are boxed. (B) Alignment of the conserved motifs of DH domains from CHL PKSs. Asterisks indicate the conserved acidic amino acid residues, and the putative mutant DH domain of module 8 is boxed. Chemistry & Biology 2006 13, 575-585DOI: (10.1016/j.chembiol.2006.03.008) Copyright © 2006 Elsevier Ltd Terms and Conditions