Odile Huchette, research engineer G&H October 2002, Montpellier WP3 / cultivation, P4 P4 : persons involved Odile Huchette, research engineer Christèle Bellamy, research technician Didier Perrin, research assistant Rémi Kahane, "co-ordinator" COOPD'OR R&D-CIRAD FLHOR

G&H October 2002, Montpellier WP3 / cultivation, P4 Workplan 2002 Milestones 3rd year : Differential display of clones exposed in vitro to various mineral nutrition treatments Greenhouse experiment in various S regimes Sampling of fresh material during the cultivation treatment for CSO analysis (P4 & P5) Jan. Feb. March April May June July Aug. Sept. Oct. Nov. Dec. VITRO Micropropagation 2nd Micropropagation Experiment with various S of 4 genotypes nutrition Experiment with V. Chovelon / L. Fereol : Effect of different factors (light source, photoperiod and medium) on in vitro bulblet production (3 varieties) Green-house Greenhouse experiment COOPD'OR R&D

Printanor (PRI), Morasol (MOR), Messidrôme (MES) G&H October 2002, Montpellier WP3 / cultivation, P4 Greenhouse experiment 2002  3 varieties : Printanor (PRI), Morasol (MOR), Messidrôme (MES)  3 Sulphur nutrition treatments: 0 (in the macro-elements) = Treatment S- 2 meq (normal) = Treatment TS 8 meq = Treament S++ COOPD'OR R&D

01/03 : Plantation of the cloves G&H October 2002, Montpellier WP3 / cultivation, P4 Greenhouse experiment 2002 01/03 : Plantation of the cloves 26/03 : first mineral nutrition (4-5 leaves) 17/06 : Harvest of Messidrôme 10-11/07 : Harvest of Morasol 16-17/07 : Harvest of Printanor Plantation in boxes with washed river sand Mineral nutrition with drop irrigation COOPD'OR R&D

 Weekly observations : Growing of the leaves and bulbs G&H October 2002, Montpellier WP3 / cultivation, P4 Greenhouse experiment 2002  Weekly observations : Growing of the leaves and bulbs  Measurements at harvest : - weight of the bulbs - alliin content (P5) - total sulfur (P2) in progress COOPD'OR R&D

Bulb growing = diameter of the bulb / diameter of the neck G&H October 2002, Montpellier WP3 / cultivation, P4 Growing of the 3 varieties Bulb growing = diameter of the bulb / diameter of the neck COOPD'OR R&D

G&H October 2002, Montpellier WP3 / cultivation, P4 G&H October 2002, Montpellier WP3 / cultivation, P4 Weight of the bulbs COOPD'OR R&D

G&H October 2002, Montpellier WP3 / cultivation, P4 Alliin content of the bulbs (P5) COOPD'OR R&D

(nmol / mg fresh matter) G&H October 2002, Montpellier WP3 / cultivation, P4 Statistical analysis  influence of the variety S++ MES MOR PRI VARIETY 51 70 89 108 127 (nmol / mg fresh matter) ALLIIN S- TS Analysis of Variance COOPD'OR R&D

(nmol / mg fresh matter) G&H October 2002, Montpellier WP3 / cultivation, P4 Statistical analysis  influence of the S treatment MES S++ S- TS TREATMENT 51 70 89 108 127 (nmol / mg fresh matter) ALLIIN MOR PRI Analysis of Variance COOPD'OR R&D

G&H October 2002, Montpellier WP3 / cultivation, P4 Milestones Year 3 Differential display of clones exposed in vitro to various mineral nutritions in progress Greenhouse experiment in various S regimes  Sampling of fresh material during the cultivation treatment for CSO analysis (P4 & P5)  COOPD'OR R&D

G&H October 2002, Montpellier WP3 / cultivation, P4 Future experiments In Vitro : Differential display of clones exposed in vitro to various mineral nutritions Effect of an absence of S in the macro-elements : role of S atmospheric ? Effect of high values of S in the medium : phytotoxicity? Interaction between S and light (quality + quantity) Interaction between S and N, or S and Se ? COOPD'OR R&D

In Greenhouse (2003) : COOPD'OR R&D G&H October 2002, Montpellier WP3 / cultivation, P4 Future experiments and Milestones Year 4 In Greenhouse (2003) : Greenhouse cultivation of garlic bulbs enriched in CSO ? Comparison between extreme genotypes low and high in S content and comparison virus-free and virus-contaminated garlic for sulphur composition CSO content ?  Greenhouse cultivation of garlic bulbs providing from the experiment in greenhouse 2002 Chemical analysis (P5) before and after processing into powder. Transfer to the health partners ? At high level of S, effect of the N level (3 mineral nutrition treatments) ? COOPD'OR R&D